Immunochemical detection of a primase activity related subunit of DNA polymerase-.alpha. from human and mouse cells using the monoclonal antibody

Biochemistry ◽  
1987 ◽  
Vol 26 (24) ◽  
pp. 7749-7754 ◽  
Author(s):  
Tatsuo Yagura ◽  
Tomoko Kozu ◽  
Takeshi Seno ◽  
Shigeaki Tanaka
Keyword(s):  
Monoclonal Antibody ◽  
Dna Polymerase ◽  
Dna Polymerase Alpha ◽  
Immunochemical Detection ◽  
Mouse Cells ◽  
Human And Mouse

scholarly journals Proliferating cells evaluated histologically with a monoclonal antibody against DNA polymerase .ALPHA. in various liver diseases.

Kanzo ◽  
1990 ◽  
Vol 31 (3) ◽  
pp. 265-271
Author(s):  
Hiroki SAKAGUCHI ◽  
Shuichi SEKI ◽  
Atsushi YANAI ◽  
Nobuyoshi KAWAKITA ◽  
Kohshun KIM ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Dna Polymerase ◽  
Liver Diseases ◽  
Proliferating Cells ◽  
Dna Polymerase Alpha

Identification and fine structure of proliferating hepatocytes in malignant and nonmalignant liver diseases by use of a monoclonal antibody against DNA polymerase alpha

1990 ◽  
Vol 21 (10) ◽  
pp. 1020-1030 ◽  
Author(s):  
Shuichi Seki ◽  
Hiroki Sakaguchi ◽  
Nobuyoshi Kawakita ◽  
Atsushi Yanai ◽  
Kohshun Kim ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Fine Structure ◽  
Dna Polymerase ◽  
Liver Diseases ◽  
Dna Polymerase Alpha

scholarly journals Identification of a higher molecular weight DNA polymerase alpha catalytic polypeptide in monkey cells by monoclonal antibody.

1984 ◽  
Vol 81 (24) ◽  
pp. 7777-7781 ◽  
Author(s):  
E. Karawya ◽  
J. Swack ◽  
W. Albert ◽  
J. Fedorko ◽  
J. D. Minna ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Monoclonal Antibody ◽  
Dna Polymerase ◽  
Dna Polymerase Alpha

Detection of proliferating hepatocytes in patients with acute hepatic failure by mitotic figures and a monoclonal antibody against DNA polymerase alpha

2008 ◽  
Vol 11 (2) ◽  
pp. 118-126 ◽  
Author(s):  
Shuichi Seki ◽  
Hiroki Sakaguchi ◽  
Nobuyoshi Kawakita ◽  
Atsushi Yanai ◽  
Tetsuo Kuroki ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Dna Polymerase ◽  
Hepatic Failure ◽  
Acute Hepatic Failure ◽  
Dna Polymerase Alpha ◽  
Mitotic Figures

scholarly journals Systematic comparison of monoclonal versus polyclonal antibodies for mapping histone modifications by ChIP-seq

2016 ◽  
Author(s):  
Michele Busby ◽  
Catherine Xue ◽  
Catherine Li ◽  
Yossi Farjoun ◽  
Elizabeth Gienger ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Histone Modifications ◽  
Polyclonal Antibodies ◽  
Post Translational Modifications ◽  
Systematic Comparison ◽  
Mouse Cells ◽  
Overall Performance ◽  
Consistent Performance ◽  
Human And Mouse

AbstractBackgroundThe robustness of ChIP-seq datasets is highly dependent upon the antibodies used. Currently, polyclonal antibodies are the standard despite several limitations: they are non-renewable, vary in performance between lots, and need to be validated with each new lot. In contrast, monoclonal antibody lots are renewable and provide consistent performance. To increase ChIP-seq standardization, we investigated whether monoclonal antibodies could replace polyclonal antibodies. We compared monoclonal antibodies that target five key histone modifications (H3K4me1, H3K4me3, H3K9me3, H3K27ac and H3K27me3) to their polyclonal counterparts in both human and mouse cells.ResultsOverall performance was highly similar for four monoclonal/polyclonal pairs, including when we used two distinct lots of the same monoclonal antibody. In contrast, the binding patterns for H3K27ac differed substantially between polyclonal and monoclonal antibodies. However, this was most likely due to the distinct immunogen used rather than the clonality of the antibody.ConclusionsAltogether, we found that monoclonal antibodies as a class perform as well as polyclonal antibodies for the detection of histone post-translational modifications in both human and mouse. Accordingly, we recommend the use of monoclonal antibodies in ChIP-seq experiments.

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