Fragmentation of rabbit skeletal muscle calsequestrin: spectral and ion-binding properties of the carboxyl-terminal region

Biochemistry ◽  
1987 ◽  
Vol 26 (23) ◽  
pp. 7458-7465 ◽  
Author(s):  
Mamoru Ohnishi ◽  
Reinhart A. F. Reithmeier
Keyword(s):  
Skeletal Muscle ◽  
Terminal Region ◽  
Rabbit Skeletal Muscle ◽  
Ion Binding ◽  
Binding Properties ◽  
Carboxyl Terminal

scholarly journals Calcium-Binding Properties of Sarcoplasmic Reticulum As Influenced by ATP, Caffeine, Quinine, and Local Anesthetics

1968 ◽  
Vol 52 (4) ◽  
pp. 622-642 ◽  
Author(s):  
Arselio P. Carvalho
Keyword(s):  
Skeletal Muscle ◽  
Sarcoplasmic Reticulum ◽  
Binding Sites ◽  
Local Anesthetics ◽  
Calcium Binding ◽  
Rabbit Skeletal Muscle ◽  
Cellular Membranes ◽  
Binding Properties ◽  
45Ca Fluxes

Calcium retained at binding sites of the sarcoplasmic reticulum membranes isolated from rabbit skeletal muscle requires 10-5 - 10-4 M ATP to exchange with 45Ca added to the medium. The ATP requirement for Ca exchangeability was observed with respect to the "intrinsic" Ca of the reticulum membranes and the fraction of Ca that is "actively" bound in the presence of ATP. Furthermore, a concentration of free Ca in the medium higher than 10-8 M is required for ATP to promote Ca exchangeability. This exchangeability is not influenced by caffeine, quinine, procaine, and tetracaine, and Ca that is either nonexchangeable (in the absence of ATP) or exchangeable (in the presence of ATP) is released by 1–5 mM quinine or tetracaine, but neither caffeine (6 mM) nor procaine (2–5 mM) has this effect. Quinine or tetracaine also releases Ca and Mg bound passively to the reticulum membranes. A possible role of ATP in maintaining the integrity of cellular membranes is discussed, and the effects of caffeine, quinine, and of local anesthetics on the binding of Ca by the isolated reticulum are related to the effects of these agents on 45Ca fluxes and on the twitch output observed in whole muscles.

scholarly journals A Carboxyl-terminal Region Important for the Expression and Targeting of the Skeletal Muscle Dihydropyridine Receptor

2000 ◽  
Vol 275 (30) ◽  
pp. 23169-23174 ◽  
Author(s):  
Catherine Proenza ◽  
Christina Wilkens ◽  
Nancy M. Lorenzon ◽  
Kurt G. Beam
Keyword(s):  
Skeletal Muscle ◽  
Dihydropyridine Receptor ◽  
Terminal Region ◽  
Carboxyl Terminal

scholarly journals Thin filament Ca2+binding properties and regulatory unit interactions alter kinetics of tension development and relaxation in rabbit skeletal muscle

2008 ◽  
Vol 586 (15) ◽  
pp. 3683-3700 ◽  
Author(s):  
Kareen L. Kreutziger ◽  
Nicoletta Piroddi ◽  
Beatrice Scellini ◽  
Chiara Tesi ◽  
Corrado Poggesi ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Thin Filament ◽  
Rabbit Skeletal Muscle ◽  
Binding Properties ◽  
Tension Development ◽  
Kinetics Of

scholarly journals Calcium-Binding Properties of Sarcoplasmic Reticulum As Influenced by ATP, Caffeine, Quinine, and Local Anesthetics

1968 ◽  
Vol 52 (3) ◽  
pp. 622-642 ◽  
Author(s):  
Arselio P. Carvalho
Keyword(s):  
Skeletal Muscle ◽  
Sarcoplasmic Reticulum ◽  
Binding Sites ◽  
Local Anesthetics ◽  
Calcium Binding ◽  
Rabbit Skeletal Muscle ◽  
Cellular Membranes ◽  
Binding Properties ◽  
45Ca Fluxes

Calcium retained at binding sites of the sarcoplasmic reticulum membranes isolated from rabbit skeletal muscle requires 10-5 – 10-4 M ATP to exchange with 45Ca added to the medium. The ATP requirement for Ca exchangeability was observed with respect to the "intrinsic" Ca of the reticulum membranes and the fraction of Ca that is "actively" bound in the presence of ATP. Furthermore, a concentration of free Ca in the medium higher than 10-8 M is required for ATP to promote Ca exchangeability. This exchangeability is not influenced by caffeine, quinine, procaine, and tetracaine, and Ca that is either nonexchangeable (in the absence of ATP) or exchangeable (in the presence of ATP) is released by 1–5 mM quinine or tetracaine, but neither caffeine (6 mM) nor procaine (2–5 mM) has this effect. Quinine or tetracaine also releases Ca and Mg bound passively to the reticulum membranes. A possible role of ATP in maintaining the integrity of cellular membranes is discussed, and the effects of caffeine, quinine, and of local anesthetics on the binding of Ca by the isolated reticulum are related to the effects of these agents on 45Ca fluxes and on the twitch output observed in whole muscles.

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