Complementary DNA and derived amino acid sequence of the .beta. subunit of human complement protein C8: identification of a close structural and ancestral relationship to the .alpha. subunit and C9

Biochemistry ◽  
1987 ◽  
Vol 26 (12) ◽  
pp. 3565-3570 ◽  
Author(s):  
O. M. Zack Howard ◽  
A. Gururaj Rao ◽  
James M. Sodetz
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Beta Subunit ◽  
Alpha Subunit ◽  
Human Complement ◽  
Complement Protein ◽  
Complementary Dna ◽  
Complement Protein C8

Complementary DNA and derived amino acid sequence of the .alpha. subunit of human complement protein C8: evidence for the existence of a separate .alpha. subunit messenger RNA

Biochemistry ◽  
1987 ◽  
Vol 26 (12) ◽  
pp. 3556-3564 ◽  
Author(s):  
A. Gururaj Rao ◽  
O. M. Zack Howard ◽  
Simon C. Ng ◽  
Alexander S. Whitehead ◽  
Harvey R. Colten ◽  
...  
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Messenger Rna ◽  
Alpha Subunit ◽  
Human Complement ◽  
Complement Protein ◽  
Complementary Dna ◽  
Complement Protein C8

scholarly journals Amino acid sequence of the human fibronectin receptor.

1987 ◽  
Vol 105 (3) ◽  
pp. 1183-1190 ◽  
Author(s):  
W S Argraves ◽  
S Suzuki ◽  
H Arai ◽  
K Thompson ◽  
M D Pierschbacher ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Calcium Binding ◽  
Beta Subunit ◽  
Alpha Subunit ◽  
Receptor Subunit ◽  
Fibronectin Receptor ◽  
Adhesion Receptor ◽  
Receptor Beta

The amino acid sequence deduced from cDNA of the human placental fibronectin receptor is reported. The receptor is composed of two subunits: an alpha subunit of 1,008 amino acids which is processed into two polypeptides disulfide bonded to one another, and a beta subunit of 778 amino acids. Each subunit has near its COOH terminus a hydrophobic segment. This and other sequence features suggest a structure for the receptor in which the hydrophobic segments serve as transmembrane domains anchoring each subunit to the membrane and dividing each into a large ectodomain and a short cytoplasmic domain. The alpha subunit ectodomain has five sequence elements homologous to consensus Ca2+-binding sites of several calcium-binding proteins, and the beta subunit contains a fourfold repeat strikingly rich in cysteine. The alpha subunit sequence is 46% homologous to the alpha subunit of the vitronectin receptor. The beta subunit is 44% homologous to the human platelet adhesion receptor subunit IIIa and 47% homologous to a leukocyte adhesion receptor beta subunit. The high degree of homology (85%) of the beta subunit with one of the polypeptides of a chicken adhesion receptor complex referred to as integrin complex strongly suggests that the latter polypeptide is the chicken homologue of the fibronectin receptor beta subunit. These receptor subunit homologies define a superfamily of adhesion receptors. The availability of the entire protein sequence for the fibronectin receptor will facilitate studies on the functions of these receptors.

scholarly journals Molecular cloning and deduced amino acid sequences of the α- and β- subunits of mammalian NAD+-isocitrate dehydrogenase

1995 ◽  
Vol 310 (3) ◽  
pp. 917-922 ◽  
Author(s):  
B J Nichols ◽  
A C F Perry ◽  
L Hall ◽  
R M Denton
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Isocitrate Dehydrogenase ◽  
Sequence Data ◽  
Amino Acid Sequences ◽  
Beta Subunit ◽  
Peptide Sequence ◽  
Alpha Subunit ◽  
Cdna Clones ◽  
Oligonucleotide Primers

A 153 bp fragment of the cDNA encoding the beta-subunit of pig heart NAD(+)-isocitrate dehydrogenase (NAD(+)-ICDH) was specifically amplified by PCR, using redundant oligonucleotide primers based on partial peptide sequence data [Huang and Colman (1990) Biochemistry 29, 8266-8273]. This PCR fragment was then used as a probe to isolate cDNA clones encoding the complete mature form of the beta-subunit from a monkey testis cDNA library. Examination of the deduced amino acid sequence of the monkey subunit and the partial sequence of the pig heart enzyme revealed a high level of sequence conservation. In addition, 3 overlapping fragments of the cDNA for the alpha-subunit of monkey NAD(+)-ICDH were amplified using oligonucleotide primers derived from the cDNA sequence of a subunit of bovine NAD(+)-ICDH (EMBL accession no: U07980). These cDNA fragments allow deduction of the amino acid sequence of the alpha-subunit. Since the gamma-subunit of monkey NAD(+)-ICDH has already been cloned [Nichols, Hall, Perry and Denton (1993) Biochem. J. 295, 347-350], a deduced amino acid sequence is now available for all three subunits of mammalian NAD(+)-ICDH. Interrelationships between these subunits are discussed and they are compared with the two subunits of yeast NAD(+)-ICDH and Escherichia coli NADP(+)-ICDH.

Nucleotide sequence of complementary DNA and derived amino acid sequence of murine complement protein C3

1984 ◽  
Vol 306 (1129) ◽  
pp. 333-344 ◽  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Transition Region ◽  
Sequence Homology ◽  
Complement Protein ◽  
Single Chain ◽  
Complementary Dna ◽  
Α Chain ◽  
Β Chain

The nucleotide sequences coding for murine complement component C3 have been determined from a cloned genomic DNA fragment and several overlapping cloned complementary DNA fragments. The amino acid sequence of the protein was deduced. The mature β and α subunits contain 642 and 993 amino acids respectively. Including a 24 amino acid signal peptide and four arginines in the β—α transition region, which are probably not contained in the mature protein, the unglycosylated single chain precursor protein preproC3 would have a molecular mass of 186484 Da and consist of 1663 amino acid residues. The C3 messenger RNA would be composed of a 56 + 2 nucleotide long 5' non-translated region, 4992 nucleotides of coding sequence, and a 3' non-translated region of 39 nucleotides, excluding the poly A tail. The β chain contains only three cysteine residues, the α chain 24, ten of which are clustered in the carboxy terminal stretch of 175 amino acids. Two potential carbohydrate attachment sites are predicted for the α chain, none for the β chain. From a comparison with human C3 cDNA sequence (of which over 80% has been determined) an extensive overall sequence homology was observed. Human and murine preproC3 would be of very similar length and share several noteworthy properties: the same order of the subunits in the precursor, the same basic residue multiplet in the β-a transition region, and a glutamine residue in the thioester region. The equivalent position of the known factor I cleavage sites in human C3a could be located in the murine C3 α chain and the size and sequence of the resulting peptide were deduced. A comparison of the amino acid sequences of murine G3 and human alpha2-macroglobulin is given. Several areas of strong sequence homology are observed, and we conclude that the two genes must have evolved from a common ancestor.

scholarly journals Riboflavin synthases of Bacillus subtilis. Purification and amino acid sequence of the alpha subunit.

1990 ◽  
Vol 265 (8) ◽  
pp. 4204-4209
Author(s):  
K Schott ◽  
J Kellermann ◽  
F Lottspeich ◽  
A Bacher
Keyword(s):  
Bacillus Subtilis ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Alpha Subunit
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