A general method of analysis of ligand-macromolecule equilibria using a spectroscopic signal from the ligand to monitor binding. Application to Escherichia coli single-strand binding protein-nucleic acid interactions

Biochemistry ◽  
1987 ◽  
Vol 26 (11) ◽  
pp. 3099-3106 ◽  
Author(s):  
Wlodzimierz Bujalowski ◽  
Timothy M. Lohman
Keyword(s):  
Escherichia Coli ◽  
Nucleic Acid ◽  
Binding Protein ◽  
Single Strand ◽  
Protein Nucleic Acid ◽  
Nucleic Acid Interactions ◽  
General Method

Folding and binding: Protein–nucleic acid interactions

2000 ◽  
Vol 10 (1) ◽  
pp. 11
Author(s):  
Philip E Bourne ◽  
Judith Murray-Rust ◽  
Jeremy H Lakey
Keyword(s):  
Nucleic Acid ◽  
Binding Protein ◽  
Protein Nucleic Acid ◽  
Nucleic Acid Interactions

Web alert Folding and binding Protein-nucleic acid interactions

1997 ◽  
Vol 7 (1) ◽  
pp. 135
Author(s):  
PhilipE Bourne ◽  
Judith Murray-Rust ◽  
JeremyH Lakey
Keyword(s):  
Nucleic Acid ◽  
Binding Protein ◽  
Protein Nucleic Acid ◽  
Nucleic Acid Interactions

scholarly journals X-ray crystallographic and NMR studies of protein-protein and protein-nucleic acid interactions involving the KH domains from human poly(C)-binding protein-2

RNA ◽  
2007 ◽  
Vol 13 (7) ◽  
pp. 1043-1051 ◽  
Author(s):  
Z. Du ◽  
J. K. Lee ◽  
S. Fenn ◽  
R. Tjhen ◽  
R. M. Stroud ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Binding Protein ◽  
Nmr Studies ◽  
X Ray ◽  
Protein Nucleic Acid ◽  
Nucleic Acid Interactions

Snapshot blotting: The transfer of nucleic acids and nucleoprotein complexes from electrophoresis gels to EM grids

1992 ◽  
Vol 50 (1) ◽  
pp. 762-763
Author(s):  
Stephen D. Jett
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Nucleic Acid Binding ◽  
Mobility Shift ◽  
Carbon Coated ◽  
Nucleoprotein Complexes ◽  
Mobility Shift Assay ◽  
Protein Nucleic Acid ◽  
Gel Mobility Shift ◽  
Nucleic Acid Interactions

The electrophoresis gel mobility shift assay is a popular method for the study of protein-nucleic acid interactions. The binding of proteins to DNA is characterized by a reduction in the electrophoretic mobility of the nucleic acid. Binding affinity, stoichiometry, and kinetics can be obtained from such assays; however, it is often desirable to image the various species in the gel bands using TEM. Present methods for isolation of nucleoproteins from gel bands are inefficient and often destroy the native structure of the complexes. We have developed a technique, called “snapshot blotting,” by which nucleic acids and nucleoprotein complexes in electrophoresis gels can be electrophoretically transferred directly onto carbon-coated grids for TEM imaging.

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