Dynamics of fd coat protein in lipid bilayers

Biochemistry ◽  
1987 ◽  
Vol 26 (3) ◽  
pp. 854-862 ◽  
Author(s):  
G. C. Leo ◽  
L. A. Colnago ◽  
K. G. Valentine ◽  
S. J. Opella
Keyword(s):  
Coat Protein ◽  
Lipid Bilayers

Spin-label electron spin resonance study of bacteriophage M13 coat protein incorporation into mixed lipid bilayers

Biochemistry ◽  
1987 ◽  
Vol 26 (24) ◽  
pp. 7571-7574 ◽  
Author(s):  
Klaas P. Datema ◽  
Cor J. A. M. Wolfs ◽  
Derek Marsh ◽  
Anthony Watts ◽  
Marcus A. Hemminga
Keyword(s):  
Electron Spin Resonance ◽  
Coat Protein ◽  
Lipid Bilayers ◽  
Electron Spin ◽  
Spin Label ◽  
Electron Spin Resonance Study ◽  
Spin Resonance ◽  
Bacteriophage M13 ◽  
Protein Incorporation ◽  
Spin Resonance Study

A small protein in model membranes: a time-resolved fluorescence and ESR study on the interaction of M13 coat protein with lipid bilayers

1992 ◽  
Vol 21 (5) ◽  
pp. 305-311 ◽  
Author(s):  
Johan C. Sanders ◽  
M. Francesca Ottaviani ◽  
Arie van Hoek ◽  
Antonie J. W. G. Visser ◽  
Marcus A. Hemminga
Keyword(s):  
Coat Protein ◽  
Lipid Bilayers ◽  
Model Membranes ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Small Protein

In SituAggregational State of M13 Bacteriophage Major Coat Protein in Sodium Cholate and Lipid Bilayers†

Biochemistry ◽  
1997 ◽  
Vol 36 (40) ◽  
pp. 12268-12275 ◽  
Author(s):  
David Stopar ◽  
Ruud B. Spruijt ◽  
Cor J. A. M. Wolfs ◽  
Marcus A. Hemminga
Keyword(s):  
Coat Protein ◽  
Lipid Bilayers ◽  
Sodium Cholate ◽  
Major Coat Protein ◽  
M13 Bacteriophage

Pf3 coat protein forms voltage-gated ion channels in planar lipid bilayers

Biochemistry ◽  
1994 ◽  
Vol 33 (1) ◽  
pp. 283-290 ◽  
Author(s):  
Michael Pawlak ◽  
Andreas Kuhn ◽  
Horst Vogel
Keyword(s):  
Ion Channels ◽  
Coat Protein ◽  
Lipid Bilayers ◽  
Planar Lipid Bilayers ◽  
Voltage Gated ◽  
Voltage Gated Ion Channels ◽  
Gated Ion Channels

scholarly journals Spin-label ESR of bacteriophage M13 coat protein in mixed lipid bilayers. Characterization of molecular selectivity of charged phospholipids for the bacteriophage M13 coat protein in lipid bilayers

Biochemistry ◽  
1989 ◽  
Vol 28 (26) ◽  
pp. 9995-10001 ◽  
Author(s):  
Cor J. A. M. Wolfs ◽  
Laszlo I. Horvath ◽  
Derek Marsh ◽  
Anthony Watts ◽  
Marcus A. Hemminga
Keyword(s):  
Coat Protein ◽  
Lipid Bilayers ◽  
Spin Label ◽  
Bacteriophage M13

Observation of Concanavalin-A receptors on hydrated planar erythrocyte membrane film by in situ electron microscopy

1983 ◽  
Vol 41 ◽  
pp. 608-609
Author(s):  
Neng-Bo He ◽  
S.W. Hui
Keyword(s):  
Lipid Bilayers ◽  
Erythrocyte Membrane ◽  
Lipid Membranes ◽  
Surface Film ◽  
Biological Membranes ◽  
Electron Microscopic Observation ◽  
Electron Microscopic ◽  
Black Lipid Membranes ◽  
Fine Mesh ◽  
Planar Films

Monolayers and planar "black" lipid membranes have been widely used as models for studying the structure and properties of biological membranes. Because of the lack of a suitable method to prepare these membranes for electron microscopic observation, their ultrastructure is so far not well understood. A method of forming molecular bilayers over the holes of fine mesh grids was developed by Hui et al. to study hydrated and unsupported lipid bilayers by electron diffraction, and to image phase separated domains by diffraction contrast. We now adapted the method of Pattus et al. of spreading biological membranes vesicles on the air-water interfaces to reconstitute biological membranes into unsupported planar films for electron microscopic study. hemoglobin-free human erythrocyte membrane stroma was prepared by hemolysis. The membranes were spreaded at 20°C on balanced salt solution in a Langmuir trough until a surface pressure of 20 dyne/cm was reached. The surface film was repeatedly washed by passing to adjacent troughs over shallow partitions (fig. 1).

Effect of Integral Proteins on Frozen Membrane Fracture

1980 ◽  
Vol 38 ◽  
pp. 756-759 ◽  
Author(s):  
S. Kirchanski ◽  
D. Branton
Keyword(s):  
Lipid Bilayers ◽  
Freeze Fracture ◽  
Covalent Bond ◽  
Erythrocyte Membranes ◽  
Glycophorin A ◽  
Experimental Protocol ◽  
Transmembrane Glycoprotein ◽  
Bond Breakage ◽  
Human Erythrocyte Membranes ◽  
Integral Proteins

We have investigated the effect of integral membrane proteins upon the fracturing of frozen lipid bilayers. This investigation has been part of an effort to develop freeze fracture labeling techniques and to assess the possible breakage of covalent protein bonds during the freeze fracture process. We have developed an experimental protocol utilizing lectin affinity columns which should detect small amounts of covalent bond breakage during the fracture of liposomes containing purified (1) glycophorin (a transmembrane glycoprotein of human erythrocyte membranes). To fracture liposomes in bulk, frozen liposomes are ground repeatedly under liquid nitrogen. Failure to detect any significant covalent bond breakage (contrary to (2)) led us to question the effectiveness of our grinding procedure in fracturing and splitting lipid bilayers.

Late Stages of the “Pearling" Instability in Lipid Bilayers

1997 ◽  
Vol 7 (9) ◽  
pp. 1185-1204 ◽  
Author(s):  
J. L. Coveas ◽  
S. T. Milner ◽  
W. B. Russel
Keyword(s):  
Lipid Bilayers ◽  
Late Stages
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