Purification and characterization of a molecular weight 100,000 coat protein from coated vesicles obtained from bovine brain

Biochemistry ◽  
1986 ◽  
Vol 25 (22) ◽  
pp. 6942-6947 ◽  
Author(s):  
Kondury Prasad ◽  
Takao Yora ◽  
Okimichi Yano ◽  
Roland E. Lippoldt ◽  
Harold Edelhoch ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Coat Protein ◽  
Bovine Brain ◽  
Coated Vesicles ◽  
Purification And Characterization

Prothrombin Metz : Purification and Characterization of a Variant of Human Prothrombin

1979 ◽  
Author(s):  
M Ribieto ◽  
J Elion ◽  
D Labie ◽  
F Josso
Keyword(s):  
Molecular Weight ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Factor Xa ◽  
Polyacrylamide Gel ◽  
Cleavage Pattern ◽  
Purification And Characterization ◽  
Double Immunodiffusion ◽  
The Family

For the purification of the abnormal prothrombin (Pt Metz), advantage has been taken of the existence in the family of three siblings who, being double heterozygotes for Pt Metz and a hypoprothrombinemia, have no normal Pt. Purification procedures included barium citrate adsorption and chromatography on DEAE Sephadex as for normal Pt. As opposed to some other variants (Pt Barcelona and Madrid), Pt Metz elutes as a single symetrical peak. By SDS polyacrylamide gel electrophoresis, this material is homogeneous and appears to have the same molecular weight as normal Pt. Comigration of normal and abnormal Pt in the absence of SDS, shows a double band suggesting an abnormal charge for the variant. Pt Metz exhibits an identity reaction with the control by double immunodiffusion. Upon activation by factor Xa, Pt Metz can generate amydolytic activity on Bz-Phe-Val-Arg-pNa (S2160), but only a very low clotting activity. Clear abnormalities are observed in the cleavage pattern of Pt Metz when monitored by SDS gel electrophoresis. The main feature are the accumulation of prethrombin l (Pl) and the appearance of abnormal intermediates migrating faster than Pl.

Characterization of [3H]5-hydroxytryptamine and [3H]spiperone binding sites in clathrin-coated vesicles from bovine brain

1998 ◽  
Vol 794 (2) ◽  
pp. 291-298 ◽  
Author(s):  
Kayoko Moroi ◽  
Naoko Ozaki ◽  
Tomoko Kadota ◽  
Ken Kadota
Keyword(s):  
Binding Sites ◽  
Bovine Brain ◽  
Coated Vesicles
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