Kinetics and extent of fusion between Sendai virus and erythrocyte ghosts: application of a mass action kinetic model

Biochemistry ◽  
1986 ◽  
Vol 25 (8) ◽  
pp. 2155-2161 ◽  
Author(s):  
Shlomo Nir ◽  
Karin Klappe ◽  
Dick Hoekstra
Keyword(s):  
Kinetic Model ◽  
Sendai Virus ◽  
Mass Action ◽  
Erythrocyte Ghosts ◽  
Mass Action Kinetic

scholarly journals Mass Action Kinetic Model of Apoptosis by TRAIL-Functionalized Leukocytes

2018 ◽  
Vol 8 ◽  
Author(s):  
Emily E. Lederman ◽  
Jacob M. Hope ◽  
Michael R. King
Keyword(s):  
Kinetic Model ◽  
Mass Action ◽  
Mass Action Kinetic

scholarly journals Bulk Fusion Studies of Sendai Virus and Application of Mass Action Kinetic Fusion Model

2021 ◽  
Vol 120 (3) ◽  
pp. 320a
Author(s):  
Papa Freduah A. Anderson ◽  
Robert J. Rawle
Keyword(s):  
Sendai Virus ◽  
Mass Action ◽  
Fusion Model ◽  
Mass Action Kinetic

The role of cell swelling and haemolysis in Sendai virus-induced cell fusion and in the diffusion of incorporated viral antigens

1980 ◽  
Vol 42 (1) ◽  
pp. 153-167
Author(s):  
S. Knutton ◽  
T. Bachi
Keyword(s):  
Cell Fusion ◽  
Erythrocyte Membrane ◽  
Sendai Virus ◽  
Viral Envelope ◽  
Cell Swelling ◽  
Haemolytic Activity ◽  
Erythrocyte Ghosts ◽  
Viral Antigens ◽  
Cell Cell

The role of the haemolytic activity of Sendai virus in cell-cell fusion has been examined in monolayers of human erythrocytes and erythrocyte ghosts fused with either haemolytic or non-haemolytic virus. Morphological observations indicate that cell swelling and haemolysis is a distinct event in cell-cell fusion irrespective of whether it is virally induced or, in the case of non-haemolytic virus, experimentally induced. Osmotic swelling appears to be the driving force by which cells which have established sites of membrane fusion expand such sites to form poly-erythrocytes. Immunofluorescent labelling of viral antigens incorporated into the erythrocyte membrane as a result of viral envelope-cell fusion indicates that diffusion of antigens in the plane of the membrane is restricted in intact erythrocytes and resealed erythrocyte ghosts but not in haemolysed erythrocytes or unsealed ghosts. A perturbation of the erythrocyte membrane resulting from osmotic lysis appears to form a prerequisite for the lateral diffusion of viral elements.

Effect of anionic polymers on fusion of Sendai virus with human erythrocyte ghosts

1992 ◽  
Vol 18 (2) ◽  
pp. 163-177 ◽  
Author(s):  
S. Ohki ◽  
K. Arnold ◽  
N. Srinivasakumar ◽  
T.D. Flanagan
Keyword(s):  
Human Erythrocyte ◽  
Sendai Virus ◽  
Erythrocyte Ghosts ◽  
Anionic Polymers

scholarly journals Sendai virus causes a rise in intracellular free Ca2+ before cell fusion

1982 ◽  
Vol 206 (3) ◽  
pp. 671-674 ◽  
Author(s):  
Maurice B. Hallett ◽  
Pinhas Fuchs ◽  
Anthony K. Campbell
Keyword(s):  
Cell Fusion ◽  
Human Erythrocyte ◽  
Maximum Rate ◽  
Sendai Virus ◽  
Erythrocyte Ghosts ◽  
Absolute Requirement

1. Sendai virus caused a large increase in the concentration of free Ca2+ within human erythrocyte ghosts detected by the Ca2+-activated photoprotein obelin. 2. The increase in intracellular [Ca2+] preceded fusion. However, fusion could also be observed in the absence of a detectable rise in intracellular free [Ca2+]. 3. It was concluded that the increase in intracellular free [Ca2+] was not an absolute requirement for cell fusion, but may be necessary to produce fusion at the maximum rate.

scholarly journals Quantitative introduction of a given macromolecule into cells by fusion with erythrocyte ghosts using a fluorescence activated cell sorter.

1978 ◽  
Vol 26 (12) ◽  
pp. 1067-1073 ◽  
Author(s):  
E Mekada ◽  
M Yamaizumi ◽  
T Uchida ◽  
Y Okada
Keyword(s):  
Light Scattering ◽  
Bovine Serum Albumin ◽  
Human Erythrocyte ◽  
Mononuclear Cells ◽  
Sendai Virus ◽  
Fluorescence Analysis ◽  
Erythrocyte Ghosts ◽  
Cell Sorter ◽  
Sorting Method ◽  
L Cells

FITC-conjugated bovine serum albumin (FITC-BSA) molecules were quantitatively introduced into human erythrocyte ghosts by gradual hemolysis. When the ghosts and L cells were fused with UV-inactivated HVJ (Sendai virus), FITC-BSA was introduced into the cytoplasm of the L cells and fluorescence could be observed inthe cells with a fluorescence microscope. A mixture of L cells and ghosts was introduced into a fluorescence activated cell sorter (FACS), which could separate the mononuclear cells on the basis of their light-scattering profile. Four distinct populations of mononuclear cells were found by fluorescence analysis. These populations were separated from the cell mixture and found to correspond to cells fused with one, two and three ghosts and unfused cells. After separation, the cells from each population could form colonies in culture. As a given macromolecule can be quantitatively introduced into erythrocyte ghosts with the FITC-BSA, after fusion of these ghosts with cells, this sorting method is useful for separating cells containing a definite number of macromolecules.

Sign in / Sign up

Export Citation Format

Share Document