Regulation of cytochrome P-450p by phenobarbital and phenobarbital-like inducers in adult rat hepatocytes in primary monolayer culture and in vivo

Biochemistry ◽  
1986 ◽  
Vol 25 (5) ◽  
pp. 1124-1133 ◽  
Author(s):  
Erin G. Schuetz ◽  
Steven A. Wrighton ◽  
Stephen H. Safe ◽  
Philip S. Guzelian
Keyword(s):  
Monolayer Culture ◽  
Rat Hepatocytes ◽  
Adult Rat ◽  
Primary Monolayer Culture ◽  
Adult Rat Hepatocytes ◽  
Primary Monolayer

scholarly journals The effect of insulin-dextran complexes on the protein synthesis in the primary monolayer culture of adult rat hepatocytes.

1984 ◽  
Vol 144 (3) ◽  
pp. 245-256
Author(s):  
TSUGUHIKO NAKAI ◽  
YASUNORI KUTSUMI ◽  
YOSHIKAZU SAKAMOTO ◽  
KOJI OIDA ◽  
SUSUMU MIYABO ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Monolayer Culture ◽  
Rat Hepatocytes ◽  
Adult Rat ◽  
Primary Monolayer Culture ◽  
Adult Rat Hepatocytes ◽  
Primary Monolayer

scholarly journals PRIMARY MONOLAYER CULTURE OF ADULT RAT HEPATOCYTES IN A SERUM-FREE SYSTEM: METABOLIC AND MORPHOLOGICAL STUDIES

1981 ◽  
Vol 2 (5) ◽  
pp. 491-500
Author(s):  
SHIRO YAMADA ◽  
TSUGUHIKO NAKAI ◽  
YASUNORI KUTSUMI ◽  
RYOYU TAKEDA ◽  
KYUICHI KURAKANE ◽  
...  
Keyword(s):  
Monolayer Culture ◽  
Rat Hepatocytes ◽  
Free System ◽  
Serum Free ◽  
Adult Rat ◽  
Primary Monolayer Culture ◽  
Adult Rat Hepatocytes ◽  
Primary Monolayer

Drug Metabolism in Adult Rat Hepatocytes in Primary Monolayer Culture

1977 ◽  
Vol 72 (6) ◽  
pp. 1232-1239 ◽  
Author(s):  
Philip S. Guzelian ◽  
D. Montgomery Bissell ◽  
Urs A. Meyer
Keyword(s):  
Drug Metabolism ◽  
Monolayer Culture ◽  
Rat Hepatocytes ◽  
Adult Rat ◽  
Primary Monolayer Culture ◽  
Adult Rat Hepatocytes ◽  
Primary Monolayer

scholarly journals Thyroid thermogenesis in adult rat hepatocytes in primary monolayer culture: direct action of thyroid hormone in vitro.

1979 ◽  
Vol 73 (3) ◽  
pp. 369-383 ◽  
Author(s):  
F Ismail-Beigi ◽  
D M Bissell ◽  
I S Edelman
Keyword(s):  
Oxygen Consumption ◽  
Thyroid Hormone ◽  
Culture Medium ◽  
Monolayer Culture ◽  
Rat Hepatocytes ◽  
Adult Rat ◽  
Primary Monolayer Culture ◽  
Adult Rat Hepatocytes ◽  
Primary Monolayer ◽  
Defined Culture

We have studied the effect of 3,5,3'-triiodothyronine (T3) on the respiration of adult rat hepatocytes in primary monolayer culture prepared from hypothyroid rat liver. After addition of T3 to the culture medium at a concentration of 2 x 10(-7) M, oxygen consumption of the cultured cells increased detectably at 24 h and was maximal at 72--96 h, relative to control cultures (38.0 +/- 1.8 vs. 25.0 +/- 1.5 microliter/h.mg protein). The thyroid-responsive enzymes, Na+ + K+-activated adenosine triphosphatase (NaK-ATPase) and alpha-glycerophosphate dehydrogenase (GPD), each exhibited increased activity in response to T3, in parallel with the change in oxygen consumption, whereas the activity of Mg-dependent ATPase was unaffected. These responses to T3 were dose dependent over similar concentration ranges, the half-maximal response for each occurring at ca 8 x 10(-10) M. In thyroid-treated cells, the observed increase in respiration was almost completely (90%) inhibited after addition of ouabain (10(-3) M) to the culture medium. It was found also that a 4-h exposure of the cultured hepatocytes to T3 was sufficient to elicit a significant thermogenic response, measured at a time (48 h later) when T3 was no longer present in the medium. The response to T3 occurred in fully defined culture medium and was independent of the presence or absence of hypothyroid rat serum, corticosterone, or insulin, and cellular ATP was unaffected by T3 in concentrations up to 2 x 10(-7) M. The findings document that adult rat hepatocytes in primary monolayer culture respond directly to thyroid hormone; the increases in respiration and NaK-ATPase activity elicited by T3 were cotemporal and apparently coordinate.

Insulin depolarization of rat hepatocytes in primary monolayer culture

1983 ◽  
Vol 244 (1) ◽  
pp. C17-C23 ◽  
Author(s):  
R. Wondergem
Keyword(s):  
Monolayer Culture ◽  
Input Resistance ◽  
Rat Hepatocytes ◽  
Metabolic Inhibitors ◽  
Stable States ◽  
Ion Conductance ◽  
Insulin Effect ◽  
Primary Monolayer Culture ◽  
Primary Monolayer

Rat hepatocytes in primary monolayer culture demonstrated two stable states of transmembrane potential (Em). Low potentials ranging from -10 to -15 mV followed impalements with glass microelectrodes, and in some cells low potentials increased spontaneously or in response to a train of intermittent current (5 nA) to stable potentials of -50 mV, which were comparable to hepatocyte Em in vivo. Adding insulin at 20 mU/ml depolarized the stable higher Em 22.4 +/- 2.6 mV (n = 6) over an 11-min interval, and input resistance increased 21.4 +/- 4.7 M omega (n = 6) during the depolarization. The insulin effect was dose dependent, because adding insulin at 0.2 mU/ml depolarized the stable high Em 5.0 +/- 1.5 mV (n = 3) and increased input resistance 6.3 +/- 1.8 M omega (n = 3). In one experiment the Em repolarized 32 min after insulin was washed out. Adding metabolic inhibitors KCN (1 mM) and 2,4-dinitrophenol (10 and 1 mM) and increasing external potassium (60 mM, with external sodium reduced equivalently) also depolarized Em, but they did not increase input resistance. Thus insulin depolarized hepatocytes from a stable high Em, which is equivalent to the Em of liver in vivo, to a stable low Em, which occurs in hepatocytes in primary monolayer culture. This hormone action is consistent with changes in membrane ion conductance, and it further demonstrates that these cells can sustain two stable states of Em.

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