Stopped-flow fluorescence studies on binding kinetics of neurotoxins with acetylcholine receptor

Biochemistry ◽  
1986 ◽  
Vol 25 (2) ◽  
pp. 395-404 ◽  
Author(s):  
Toshiya Endo ◽  
Mamoru Nakanishi ◽  
Shoei Furukawa ◽  
Francois J. Joubert ◽  
Nobuo Tamiya
Keyword(s):  
Acetylcholine Receptor ◽  
Binding Kinetics ◽  
Stopped Flow ◽  
Fluorescence Studies ◽  
Kinetics Of

Stopped-flow fluorescence studies on binding of snake neurotoxins to acetylcholine receptor

1987 ◽  
Vol 6 (3) ◽  
Author(s):  
Toshiya Endo ◽  
Mamoru Nakanishi ◽  
Shoei Furukawa ◽  
FrancoisJ. Joubert ◽  
Nobuo Tamiya ◽  
...  
Keyword(s):  
Acetylcholine Receptor ◽  
Stopped Flow ◽  
Fluorescence Studies ◽  
Snake Neurotoxins

scholarly journals Binding Kinetics of 4-Methylumbelliferyl alpha-Mannobioside to Concanavalin A by Fluorescence Stopped-Flow Measurements

1980 ◽  
Vol 103 (2) ◽  
pp. 313-321 ◽  
Author(s):  
Anita LANDSCHOOT ◽  
Frank G. LOONTIENS ◽  
Robert M. CLEGG ◽  
Thomas M. JOVIN
Keyword(s):  
Concanavalin A ◽  
Binding Kinetics ◽  
Stopped Flow ◽  
Flow Measurements ◽  
Kinetics Of

scholarly journals Pseudomonas cytochrome C-551 peroxidase. A purification procedure and study of CO-binding kinetics

1983 ◽  
Vol 209 (3) ◽  
pp. 701-707 ◽  
Author(s):  
N Foote ◽  
A C Thompson ◽  
D Barber ◽  
C Greenwood
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Cytochrome C ◽  
Isoelectric Focusing ◽  
Flash Photolysis ◽  
Cytochrome C Peroxidase ◽  
Acid Precipitation ◽  
Binding Kinetics ◽  
Stopped Flow ◽  
Purification Procedure ◽  
Kinetics Of

A procedure is described for the purification of cytochrome c peroxidase from Pseudomonas aeruginosa involving extraction by sonication, followed by acid precipitation and chromatography on only two types of gel. The final preparation had a purity ratio A407/A280 of 4.2, and was found to be essentially pure by isoelectric focusing. The enzyme was shown to be unstable during degassing under vacuum except in the presence of detergent. The kinetics of CO binding to dithionite-reduced peroxidase were studied with stopped-flow and flash-photolysis techniques, and the results obtained between pH 5 and 7 suggest the existence of two forms of dithionite-reduced enzyme in slow equilibrium.

The kinetics of calcium binding to calmodulin: Quin 2 and ANS stopped-flow fluorescence studies

1984 ◽  
Vol 120 (1) ◽  
pp. 185-191 ◽  
Author(s):  
Peter Bayley ◽  
Peter Ahlstrőm ◽  
Stephen R. Martin ◽  
Sture Forsen
Keyword(s):  
Calcium Binding ◽  
Stopped Flow ◽  
Fluorescence Studies ◽  
Kinetics Of

Binding kinetics of fluorescent bisphosphonates as a tool for monitoring bone dynamics in vivo

2013 ◽  
Author(s):  
Robert Tower ◽  
Graeme Campbell ◽  
Marc Muller ◽  
Olga Will ◽  
Frederieka Grundmann ◽  
...  
Keyword(s):  
Binding Kinetics ◽  
Kinetics Of ◽  
Bone Dynamics

Influence of Brain Gangliosides on the Formation and Properties of Supported Lipid Bilayers for Binding Kinetics Studies

2018 ◽  
Author(s):  
Luke Jordan ◽  
Nathan Wittenberg
Keyword(s):  
Lipid Bilayers ◽  
Binding Kinetics ◽  
Supported Lipid Bilayers ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Supported Lipid Bilayer ◽  
Head Groups ◽  
Lipid Diffusion ◽  
Kinetics Of ◽  
Brain Gangliosides

This is a comprehensive study of the effects of the four major brain gangliosides (GM1, GD1b, GD1a, and GT1b) on the adsorption and rupture of phospholipid vesicles on SiO2 surfaces for the formation of supported lipid bilayer (SLB) membranes. Using quartz crystal microbalance with dissipation monitoring (QCM-D) we show that gangliosides GD1a and GT1b significantly slow the SLB formation process, whereas GM1 and GD1b have smaller effects. This is likely due to the net ganglioside charge as well as the positions of acidic sugar groups on ganglioside glycan head groups. Data is included that shows calcium can accelerate the formation of ganglioside-rich SLBs. Using fluorescence recovery after photobleaching (FRAP) we also show that the presence of gangliosides significantly reduces lipid diffusion coefficients in SLBs in a concentration-dependent manner. Finally, using QCM-D and GD1a-rich SLB membranes we measure the binding kinetics of an anti-GD1a antibody that has similarities to a monoclonal antibody that is a hallmark of a variant of Guillain-Barre syndrome.

scholarly journals CO Binding Kinetics of Human Cytochrome P450 3A4

1995 ◽  
Vol 270 (10) ◽  
pp. 5014-5018 ◽  
Author(s):  
Aditya P. Koley ◽  
Jeroen T. M. Buters ◽  
Richard C. Robinson ◽  
Allen Markowitz ◽  
Fred K. Friedman
Keyword(s):  
Cytochrome P450 ◽  
Binding Kinetics ◽  
Cytochrome P450 3A4 ◽  
Human Cytochrome ◽  
Kinetics Of
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