Large-scale overproduction and rapid purification of the Escherichia coli ssb gene product. Expression of the ssb gene under .lambda. PL control

Biochemistry ◽  
1986 ◽  
Vol 25 (1) ◽  
pp. 21-25 ◽  
Author(s):  
Timothy M. Lohman ◽  
J. Michael Green ◽  
Richard S. Beyer
Keyword(s):  
Escherichia Coli ◽  
Gene Product ◽  
Large Scale ◽  
Product Expression ◽  
Rapid Purification

scholarly journals Large-scale purification and characterization of the Escherichia coli rep gene product

1989 ◽  
Vol 264 (17) ◽  
pp. 10139-10147 ◽  
Author(s):  
T M Lohman ◽  
K Chao ◽  
J M Green ◽  
S Sage ◽  
G T Runyon
Keyword(s):  
Escherichia Coli ◽  
Gene Product ◽  
Large Scale ◽  
Purification And Characterization

scholarly journals Iron Acquisition of Urinary Tract Infection Escherichia coli Involves Pathogenicity in Caenorhabditis elegans

2021 ◽  
Vol 9 (2) ◽  
pp. 310
Author(s):  
Masayuki Hashimoto ◽  
Yi-Fen Ma ◽  
Sin-Tian Wang ◽  
Chang-Shi Chen ◽  
Ching-Hao Teng
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Caenorhabditis Elegans ◽  
Large Scale ◽  
Iron Acquisition ◽  
Infection Model ◽  
High Throughput Analysis ◽  
E Coli ◽  
C Elegans ◽  
Tract Infections

Uropathogenic Escherichia coli (UPEC) is a major bacterial pathogen that causes urinary tract infections (UTIs). The mouse is an available UTI model for studying the pathogenicity; however, Caenorhabditis elegans represents as an alternative surrogate host with the capacity for high-throughput analysis. Then, we established a simple assay for a UPEC infection model with C. elegans for large-scale screening. A total of 133 clinically isolated E. coli strains, which included UTI-associated and fecal isolates, were applied to demonstrate the simple pathogenicity assay. From the screening, several virulence factors (VFs) involved with iron acquisition (chuA, fyuA, and irp2) were significantly associated with high pathogenicity. We then evaluated whether the VFs in UPEC were involved in the pathogenicity. Mutants of E. coli UTI89 with defective iron acquisition systems were applied to a solid killing assay with C. elegans. As a result, the survival rate of C. elegans fed with the mutants significantly increased compared to when fed with the parent strain. The results demonstrated, the simple assay with C. elegans was useful as a UPEC infectious model. To our knowledge, this is the first report of the involvement of iron acquisition in the pathogenicity of UPEC in a C. elegans model.

scholarly journals In vitro heme O synthesis by the cyoE gene product from Escherichia coli.

1993 ◽  
Vol 268 (35) ◽  
pp. 26041-26044
Author(s):  
K Saiki ◽  
T Mogi ◽  
K Ogura ◽  
Y Anraku
Keyword(s):  
Escherichia Coli ◽  
Gene Product
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