Characterization of glucocorticoid receptor in HeLa-S3 cells

Biochemistry ◽  
1985 ◽  
Vol 24 (25) ◽  
pp. 7348-7356 ◽  
Author(s):  
H. Hoschutzky ◽  
O. Pongs
Keyword(s):  
Glucocorticoid Receptor ◽  
Hela S3 ◽  
Hela S3 Cells

scholarly journals Physical and biochemical properties of mammalian DNase X proteins: non-AUG translation initiation of porcine and bovine mRNAs for DNase X

2005 ◽  
Vol 392 (3) ◽  
pp. 511-517 ◽  
Author(s):  
Daisuke Shiokawa ◽  
Yukari Shika ◽  
Kazuki Saito ◽  
Kosuke Yamazaki ◽  
Sei-ichi Tanuma
Keyword(s):  
Translation Initiation ◽  
Biochemical Properties ◽  
Glycosylation Site ◽  
Hydrophobic Domain ◽  
Gene Expression Analyses ◽  
Hela S3 ◽  
Hela S3 Cells ◽  
Muscular Tissues ◽  
High Expression Level

DNase X is the first human DNase protein identified as being homologous with DNase I. In the present study we describe the isolation of several mammalian DNase X cDNAs and the molecular characterization of their coding proteins. A sequence comparison reveals some conserved characteristics: all the mammalian DNase X proteins have an N-terminal signal peptide, a potential N-linked glycosylation site and a C-terminal hydrophobic domain. Human DNase X, ectopically expressed in HeLa S3 cells, is located in the ER (endoplasmic reticulum) and is modified by an N-linked glycosylation at Asn-243. Gene expression analyses show that the high expression level in muscular tissues, a known feature of human DNASE X, is also observed in mouse DNase X. Interestingly, the translation of porcine and bovine DNase X proteins occurs in the absence of an in-frame AUG initiation codon. We show that their mRNAs utilize a conserved CUG triplet for translation initiation.

scholarly journals Partial fractionation and characterization of nuclear protein kinases in HeLa S3 cells

1979 ◽  
Vol 8 (4) ◽  
pp. 305-321 ◽  
Author(s):  
Judith A. Thomson ◽  
Manuel J. Mon ◽  
Janet L. Stein ◽  
Kathryn A. Duval ◽  
Lewis J. Kleinsmith ◽  
...  
Keyword(s):  
Protein Kinases ◽  
Nuclear Protein ◽  
Hela S3 ◽  
Hela S3 Cells
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