Motion of myosin cross-bridges in skeletal muscle fibers studied by time-resolved fluorescence anisotropy decay

Biochemistry ◽  
1985 ◽  
Vol 24 (14) ◽  
pp. 3731-3735 ◽  
Author(s):  
Thomas P. Burghardt ◽  
Nancy L. Thompson
Keyword(s):  
Skeletal Muscle ◽  
Fluorescence Anisotropy ◽  
Muscle Fibers ◽  
Time Resolved Fluorescence ◽  
Fluorescence Anisotropy Decay ◽  
Time Resolved ◽  
Skeletal Muscle Fibers ◽  
Anisotropy Decay ◽  
Cross Bridges

Orientational distribution of DPH in lipid membranes: a comparison of molecular dynamics calculations and experimental time-resolved anisotropy experiments

2019 ◽  
Vol 21 (14) ◽  
pp. 7594-7604 ◽  
Author(s):  
Markéta Paloncýová ◽  
Marcel Ameloot ◽  
Stefan Knippenberg
Keyword(s):  
Molecular Dynamics ◽  
Fluorescence Anisotropy ◽  
Lipid Membranes ◽  
Time Resolved Fluorescence ◽  
Fluorescence Anisotropy Decay ◽  
Time Resolved ◽  
Anisotropy Decay ◽  
Molecular Dynamics Calculations ◽  
Orientational Distribution

The behavior of the fluorescent probe diphenylhexatriene (DPH) in different lipid phases is investigated. The rotational autocorrelation functions are calculated in order to model the time-resolved fluorescence anisotropy decay. The role of the order parameters is discussed.

Local Environment of Surface-Polyelectrolyte-Bound DNA Oligomers

2000 ◽  
Vol 651 ◽  
Author(s):  
Sangmin Jeon ◽  
Sung Chul Bae ◽  
Jiang John Zhao ◽  
Steve Granick
Keyword(s):  
Aqueous Solution ◽  
Salt Concentration ◽  
Fluorescence Anisotropy ◽  
Local Environment ◽  
Time Resolved Fluorescence ◽  
Surface Complexes ◽  
Dna Oligomers ◽  
Time Resolved ◽  
Two Photon ◽  
Anisotropy Decay

AbstractTwo-photon time-resolved fluorescence anisotropy methods were used to study the dynamical environment when fluorescent-labelled DNA oligomers (labelled with FAM, 6-fluorescein-6-carboxamido hexanoate) formed surface complexes with quaternized polyvinylpyridine (QPVP) cationic layers on a glass surface. We compared the anisotropy decay of DNA in bulk aqueous solution, DNA adsorbed onto QPVP, and QPVP-DNA-QPVP sandwich structures. When DNA was adsorbed onto QPVP, its anisotropy decay was dramatically retarded compared to the bulk, which means it had very slow rotational motion on the surface. Motions slowed down with increasing salt concentration up to a level of 0.1 M NaCl, but mobility began to increase at still higher salt concentration owing to detachment from the surface-immobilizing QPVP layers.

scholarly journals Backward Movements of Cross-Bridges by Application of Stretch and by Binding of MgADP to Skeletal Muscle Fibers in the Rigor State as Studied by X-Ray Diffraction

1999 ◽  
Vol 76 (4) ◽  
pp. 1770-1783 ◽  
Author(s):  
Yasunori Takezawa ◽  
Duck-Sool Kim ◽  
Masaki Ogino ◽  
Yasunobu Sugimoto ◽  
Takakazu Kobayashi ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Fibers ◽  
X Ray Diffraction ◽  
X Ray ◽  
Rigor State ◽  
Skeletal Muscle Fibers ◽  
Cross Bridges
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