Spectroscopic assessment of secondary and tertiary structure in myelin basic protein

Biochemistry ◽  
1985 ◽  
Vol 24 (8) ◽  
pp. 1998-2004 ◽  
Author(s):  
Cynthia S. Randall ◽  
Robert Zand
Keyword(s):  
Myelin Basic Protein ◽  
Basic Protein ◽  
Tertiary Structure ◽  
Secondary And Tertiary Structure

N.M.R. studies on myelin basic protein. II. 1H N.M.R. studies of the protein and constituent peptides in aqueous solutions

1978 ◽  
Vol 31 (11) ◽  
pp. 2387 ◽  
Author(s):  
LAT Littlemore
Keyword(s):  
Chemical Shift ◽  
Myelin Basic Protein ◽  
Cathepsin D ◽  
Ring Current ◽  
Basic Protein ◽  
Tertiary Structure ◽  
Pulse Sequence ◽  
Chemical Shift Assignments ◽  
Peptide Fragment ◽  
Line Widths

1H N.M.R. spectra (270 MHz) of myelin basic protein (MBP) at pD 3.7 in D2O were obtained as a function of concentration and compared with computed spectra. Reduced line widths obtained for 0.5-mM samples and use of the convolution difference technique enabled detection of chemical shift heterogeneities for histidine, tyrosine, methionine, threonine, and isoleucine residues in the protein; this is indicative of secondary/tertiary structure. Chemical shift assignments were confirmed by the use of the Carr-Purcell A pulse sequence and selective decoupling as well as by correlation of the MBP spectrum with that of its constituent cathepsin D digest peptides. The methyl resonance from the unique methylated arginine-107 was found, and its chemical shift compared to that of NG-monomethyl-L-arginine and the methylated arginine peak in the peptide fragment, residues 90-170. The absence of ring- current effects on the methyl chemical shift precludes conformations of MBP in which the methylarginine interacts with the phenylalanine pair at residues 89 and 90.

scholarly journals Myelin basic protein enhances axonal regeneration from neural progenitor cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Zhengjian Yan ◽  
Lei Chu ◽  
Xiaojiong Jia ◽  
Lu Lin ◽  
Si Cheng
Keyword(s):  
Myelin Basic Protein ◽  
Neurite Outgrowth ◽  
Progenitor Cells ◽  
Axonal Regeneration ◽  
Neural Progenitor Cells ◽  
Basic Protein ◽  
Neural Progenitor ◽  
Dependent Manner ◽  
Cord Injury

Abstract Introduction Stem cell therapy using neural progenitor cells (NPCs) shows promise in mitigating the debilitating effects of spinal cord injury (SCI). Notably, myelin stimulates axonal regeneration from mammalian NPCs. This led us to hypothesize that myelin-associated proteins may contribute to axonal regeneration from NPCs. Methods We conducted an R-based bioinformatics analysis to identify key gene(s) that may participate in myelin-associated axonal regeneration from murine NPCs, which identified the serine protease myelin basic protein (Mbp). We employed E12 murine NPCs, E14 rat NPCs, and human iPSC-derived Day 1 NPCs (D1 hNPCs) with or without CRISPR/Cas9-mediated Mbp knockout in combination with rescue L1-70 overexpression, constitutively-active VP16-PPARγ2, or the PPARγ agonist ciglitazone. A murine dorsal column crush model of SCI utilizing porous collagen-based scaffolding (PCS)-seeded murine NPCs with or without stable Mbp overexpression was used to assess locomotive recovery and axonal regeneration in vivo. Results Myelin promotes axonal outgrowth from NPCs in an Mbp-dependent manner and that Mbp’s stimulatory effects on NPC neurite outgrowth are mediated by Mbp’s production of L1-70. Furthermore, we determined that Mbp/L1-70’s stimulatory effects on NPC neurite outgrowth are mediated by PPARγ-based repression of neuron differentiation-associated gene expression and PPARγ-based Erk1/2 activation. In vivo, PCS-seeded murine NPCs stably overexpressing Mbp significantly enhanced locomotive recovery and axonal regeneration in post-SCI mice. Conclusions We discovered that Mbp supports axonal regeneration from mammalian NPCs through the novel Mbp/L1cam/Pparγ signaling pathway. This study suggests that bioengineered, NPC-based interventions can promote axonal regeneration and functional recovery post-SCI.

scholarly journals The N terminus of human myelin basic protein consists of C2, C4, C6, and C8 alkyl carboxylic acids.

1992 ◽  
Vol 267 (14) ◽  
pp. 9779-9782
Author(s):  
M.A. Moscarello ◽  
H Pang ◽  
C.R. Pace-Asciak ◽  
D.D. Wood
Keyword(s):  
Myelin Basic Protein ◽  
Carboxylic Acids ◽  
Basic Protein ◽  
N Terminus
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