Catalytic utilization of eIF-2 and mRNA binding proteins are limiting in lysates from vesicular stomatitis virus infected L cells

Biochemistry ◽  
1984 ◽  
Vol 23 (25) ◽  
pp. 6184-6190 ◽  
Author(s):  
Ella Dratewka-Kos ◽  
Ibolya Kiss ◽  
Jean Lucas-Lenard ◽  
Harshvardhan B. Mehta ◽  
Charles L. Woodley ◽  
...  
Keyword(s):  
Vesicular Stomatitis Virus ◽  
Binding Proteins ◽  
L Cells ◽  
Mrna Binding Proteins ◽  
Vesicular Stomatitis ◽  
Mrna Binding

scholarly journals The endoplasmic reticulum-associated mRNA-binding proteins ERBP1 and ERBP2 interact in bloodstream-form Trypanosoma brucei

2019 ◽  
Author(s):  
Kathrin Bajak ◽  
Kevin Leiss ◽  
Christine Clayton ◽  
Esteban Erben
Keyword(s):  
Endoplasmic Reticulum ◽  
Ribosomal Protein ◽  
Trypanosoma Brucei ◽  
Binding Proteins ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Bloodstream Form ◽  
Control Of Gene Expression ◽  
Mrna Binding Proteins ◽  
Mrna Binding

AbstractKinetoplastids rely heavily on post-transcriptional mechanisms for control of gene expression, and on RNA-binding proteins that regulate mRNA splicing, translation and decay. Trypanosoma brucei ERBP1 (Tb927.10.14150) and ERBP2 (Tb927.9.9550) were previously identified as mRNA binding proteins that lack canonical RNA-binding domains. We here show that ERBP1 is associated with the endoplasmic reticulum, like ERBP2, and that the two proteins interact in vivo. Loss of ERBP1 from bloodstream-form T. brucei initially resulted in a growth defect but proliferation was restored after more prolonged cultivation. Results from a pull-down of tagged ERBP1 suggest that it preferentially binds to ribosomal protein mRNAs. The ERBP1 sequence resembles that of Saccharomyces cerevisiae Bfr1, which also localises to the endoplasmic reticulum and binds to ribosomal protein mRNAs. However, unlike Bfr1, ERBP1 does not bind to mRNAs encoding secreted proteins, and it is also not recruited to stress granules after starvation.

scholarly journals Humoral Autoimmune Responses to Insulin-Like Growth Factor II mRNA-Binding Proteins IMP1 and p62/IMP2 in Ovarian Cancer

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Xinxin Liu ◽  
Hua Ye ◽  
Liuxia Li ◽  
Wenjie Li ◽  
Yi Zhang ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Growth Factor ◽  
Binding Proteins ◽  
Immunofluorescence Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Insulin Like Growth Factor ◽  
Mrna Binding Proteins ◽  
Normal Individuals ◽  
Factor Ii ◽  
Mrna Binding

Ovarian cancer is one of the leading causes of cancer-related deaths among women. There is an urgent need of better approaches for the identification of appropriate biomarkers in the early detection of ovarian cancer. The aim of this study was to elucidate the significance of autoantibodies against insulin-like growth factor II mRNA-binding proteins (IMPs) in patients with ovarian cancer. In this study, autoantibody responses to two members (IMP1 and p62/IMP2) of IMPs were evaluated by enzyme-linked immunosorbent assay (ELISA), western blotting, and indirect immunofluorescence assay in sera from patients with ovarian cancer and normal human individuals. The results have demonstrated that both IMP1 and p62/IMP2 can induce relatively higher frequency of autoantibody responses in patients with ovarian cancer (26.5% and 29.4%) compared to normal individuals(P<0.01). Our preliminary data suggest that IMP1 and p62/IMP2 can stimulate autoimmune responses in ovarian cancer, and anti-IMP1 and anti-p62/IMP2 autoantibodies could be used as potential biomarkers in immunodiagnosis of ovarian cancer.

scholarly journals Insulin-like growth factor 2 mRNA-binding proteins (IGF2BPs): post-transcriptional drivers of cancer progression?

2012 ◽  
Vol 70 (15) ◽  
pp. 2657-2675 ◽  
Author(s):  
Jessica L. Bell ◽  
Kristin Wächter ◽  
Britta Mühleck ◽  
Nikolaos Pazaitis ◽  
Marcel Köhn ◽  
...  
Keyword(s):  
Growth Factor ◽  
Cancer Progression ◽  
Binding Proteins ◽  
Insulin Like Growth Factor ◽  
Mrna Binding Proteins ◽  
Mrna Binding

scholarly journals beta-Actin mRNA-binding proteins associated with the cytoskeletal framework

1993 ◽  
Vol 212 (1) ◽  
pp. 217-225 ◽  
Author(s):  
Kathryn SHARPLESS ◽  
Diane BIEGEL ◽  
Tao YANG ◽  
Joel S. PACHTER
Keyword(s):  
Binding Proteins ◽  
Beta Actin ◽  
Mrna Binding Proteins ◽  
Actin Mrna ◽  
Mrna Binding

scholarly journals Cooperation of Two mRNA-Binding Proteins Drives Metabolic Adaptation to Iron Deficiency

2008 ◽  
Vol 7 (6) ◽  
pp. 555-564 ◽  
Author(s):  
Sergi Puig ◽  
Sandra V. Vergara ◽  
Dennis J. Thiele
Keyword(s):  
Iron Deficiency ◽  
Binding Proteins ◽  
Metabolic Adaptation ◽  
Mrna Binding Proteins ◽  
Mrna Binding

scholarly journals The Double Lives of Shuttling mRNA Binding Proteins

Cell ◽  
2000 ◽  
Vol 102 (2) ◽  
pp. 135-138 ◽  
Author(s):  
Ann-Bin Shyu ◽  
Miles F Wilkinson
Keyword(s):  
Binding Proteins ◽  
Mrna Binding Proteins ◽  
Mrna Binding
Sign in / Sign up

Export Citation Format

Share Document