Complete amino acid sequence of the heavy-chain variable region from an A/J mouse antigen-nonbinding monoclonal antibody bearing the predominant arsonate idiotype

Biochemistry ◽  
1984 ◽  
Vol 23 (20) ◽  
pp. 4726-4732 ◽  
Author(s):  
John A. Smith ◽  
Michael N. Margolies
Keyword(s):  
Monoclonal Antibody ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Heavy Chain ◽  
Variable Region ◽  
Heavy Chain Variable Region ◽  
Complete Amino Acid Sequence

Amino acid sequence of the heavy-chain variable region of the crystallizable human myeloma protein Dob

Biochemistry ◽  
1979 ◽  
Vol 18 (19) ◽  
pp. 4068-4080 ◽  
Author(s):  
Lisa A. Steiner ◽  
Angeles Garcia-Pardo ◽  
Michael N. Margolies
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Heavy Chain ◽  
Variable Region ◽  
Myeloma Protein ◽  
Heavy Chain Variable Region

The Complete Amino-Acid Sequence of the Heavy Chain of the Human Myeloma Protein WIE, an Immunoglobulin D

1991 ◽  
Vol 372 (2) ◽  
pp. 795-818 ◽  
Author(s):  
R. Joachim FRIEDRICH ◽  
Rolf BÄTGE ◽  
Iris SCHRANNER ◽  
Ulrike KOTUCHA ◽  
Klaus ECKART ◽  
...  
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Heavy Chain ◽  
Myeloma Protein ◽  
Complete Amino Acid Sequence ◽  
Immunoglobulin D

scholarly journals Kinesin family in murine central nervous system.

1992 ◽  
Vol 119 (5) ◽  
pp. 1287-1296 ◽  
Author(s):  
H Aizawa ◽  
Y Sekine ◽  
R Takemura ◽  
Z Zhang ◽  
M Nangaku ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Heavy Chain ◽  
Motor Domain ◽  
Globular Domain ◽  
Complete Amino Acid Sequence ◽  
Kinesin Heavy Chain ◽  
Kinesin Family

In neuronal axons, various kinds of membranous components are transported along microtubules bidirectionally. However, only two kinds of mechanochemical motor proteins, kinesin and brain dynein, had been identified as transporters of membranous organelles in mammalian neurons. Recently, a series of genes that encode proteins closely related to kinesin heavy chain were identified in several organisms including Schizosaccharomyces pombe, Aspergillus niddulans, Saccharomyces cerevisiae, Caenorhabditus elegans, and Drosophila. Most of these members of the kinesin family are implicated in mechanisms of mitosis or meiosis. To address the mechanism of intracellular organelle transport at a molecular level, we have cloned and characterized five different members (KIF1-5), that encode the microtubule-associated motor domain homologous to kinesin heavy chain, in murine brain tissue. Homology analysis of amino acid sequence indicated that KIF1 and KIF5 are murine counterparts of unc104 and kinesin heavy chain, respectively, while KIF2, KIF3, and KIF4 are as yet unidentified new species. Complete amino acid sequence of KIF3 revealed that KIF3 consists of NH2-terminal motor domain, central alpha-helical rod domain, and COOH-terminal globular domain. Complete amino acid sequence of KIF2 revealed that KIF2 consists of NH2-terminal globular domain, central motor domain, and COOH-terminal alpha-helical rod domain. This is the first identification of the kinesin-related protein which has its motor domain at the central part in its primary structure. Northern blot analysis revealed that KIF1, KIF3, and KIF5 are expressed almost exclusively in murine brain, whereas KIF2 and KIF4 are expressed in brain as well as in other tissues. All these members of the kinesin family are expressed in the same type of neurons, and thus each one of them may transport its specific organelle in the murine central nervous system.

scholarly journals Completion of the analysis of the primary structure of the variable domain of a homogeneous rabbit antibody to type III pneumococcal polysaccharide

1974 ◽  
Vol 143 (3) ◽  
pp. 723-732 ◽  
Author(s):  
Jean-Claude Jaton
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Guinea Pig ◽  
Heavy Chain ◽  
Variable Region ◽  
Complete Sequence ◽  
Rabbit Antibody ◽  
Type Iii ◽  
Minor Variant ◽  
V Region

The amino acid sequence between residues 70 and 116 of the V (variable) region of the H (heavy) chain derived from rabbit antibody BS-5, specific for type III pneumococcal polysaccharide, was determined. The sequence of this section of the H chain which includes the hypervariable residues 94 to about 112 was unique, although minor variant sequences present in the H chain preparation would not have been detected by the techniques used in this work. Taken together with the known sequences of the N-terminal 69 residues of H chain BS-5 (Jaton & Braun, 1972) and of the V region of the light chain (Jaton, 1974b), the data establish the complete sequence of the V domain of a rabbit immunoglobulin G. The V region of H chain BS-5 is compared with the basic sequences of the three human V region subgroups known to date, with one mouse H chain, and with guinea-pig pooled H chains. Even though chains from guinea pig and mouse clearly belong to the subgroup III of variability (VHIII), rabbit H chain BS-5 (allotypic variant a1) appears more closely related to the subgroup VHII than to the subgroups VHIII or VHI. The homology between VL and VH regions of antibody BS-5 (28%) is not greater than that observed between the VH region of antibody BS-5 and the VL regions of different rabbit antibodies.

scholarly journals The amino acid sequences of the Fd fragments of two human γ 1 heavy chains

1970 ◽  
Vol 117 (4) ◽  
pp. 641-660 ◽  
Author(s):  
E. M. Press ◽  
N. M. Hogg
Keyword(s):  
Amino Acid ◽  
Aspartic Acid ◽  
Heavy Chain ◽  
Variable Region ◽  
Amino Acid Sequences ◽  
Light Chains ◽  
Aspartic Acid Residue ◽  
Variable Regions ◽  
Heavy Chain Variable Region ◽  
Heavy Chains

The amino acid sequences of the Fd fragments of two human pathological immunoglobulins of the immunoglobulin G1 class are reported. Comparison of the two sequences shows that the heavy-chain variable regions are similar in length to those of the light chains. The existence of heavy chain variable region subgroups is also deduced, from a comparison of these two sequences with those of another γ 1 chain, Eu, a μ chain, Ou, and the partial sequence of a fourth γ 1 chain, Ste. Carbohydrate has been found to be linked to an aspartic acid residue in the variable region of one of the γ 1 chains, Cor.

scholarly journals Complete amino acid sequence of the delta heavy chain of human immunoglobulin D.

1982 ◽  
Vol 79 (9) ◽  
pp. 2850-2854 ◽  
Author(s):  
N. Takahashi ◽  
D. Tetaert ◽  
B. Debuire ◽  
L. C. Lin ◽  
F. W. Putnam
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Heavy Chain ◽  
Human Immunoglobulin ◽  
Complete Amino Acid Sequence ◽  
Immunoglobulin D
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