Conformation of double-stranded DNA during agarose gel electrophoresis: fractionation of linear and circular molecules with molecular weights between 3 .times. 106 and 26 .times. 106

Biochemistry ◽  
1984 ◽  
Vol 23 (5) ◽  
pp. 922-927 ◽  
Author(s):  
Philip Serwer ◽  
Jerry L. Allen
Keyword(s):  
Gel Electrophoresis ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Molecular Weights ◽  
Double Stranded Dna

The extraction, characterization andin vitrotranslation of RNA from adultSchistosoma mansoni

Parasitology ◽  
1982 ◽  
Vol 84 (2) ◽  
pp. 253-261 ◽  
Author(s):  
M. P. R. Tenniswood ◽  
A. J. G. Simpson
Keyword(s):  
Schistosoma Mansoni ◽  
Lithium Chloride ◽  
Gel Electrophoresis ◽  
Rabbit Reticulocyte Lysate ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Molecular Weights ◽  
Reticulocyte Lysate ◽  
Urea Method

SUMMARYWe have extracted RNA fromSchistosoma mansoniusing the lithium chloride–urea method which gives good yields of undegraded RNA. The results of agarose gel electrophoresis of RNA extracted by this procedure suggest thatS. mansonihas anin vivonick in the large rRNA sub-unit. Translation of the RNA in a rabbit reticulocyte lysate gave significant incorporation of [35S]methionine into synthesized proteins. Immuno-precipitation of these translation products using a hyperimmune monkey serum sedimented between 5 and 8% of the radioactivity, which appeared to be present in approximately 13 proteins of molecular weights 18, 20, 21, 22, 23, 35, 40, 54, 60, 70, 74, 78 and 105 K Daltons.

Pulsed field agarose gel electrophoresis in the study of morphogenesis: Packaging of double-stranded DNA in the capsids of bacteriophages

1993 ◽  
Vol 14 (1) ◽  
pp. 271-277 ◽  
Author(s):  
Philip Serwer ◽  
Shirley J. Hayes ◽  
Elena T. Moreno ◽  
Donna Louie ◽  
Robert H. Watson ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Pulsed Field ◽  
Double Stranded Dna

Notizen: The Structural Components of Hog Cholera Virus

1977 ◽  
Vol 32 (5-6) ◽  
pp. 456-458 ◽  
Author(s):  
P.-J. Enzmann ◽  
Hilde Rehberg
Keyword(s):  
Molecular Weight ◽  
Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Structural Proteins ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Structural Components ◽  
Molecular Weights ◽  
Hog Cholera ◽  
Hog Cholera Virus

Abstract Hog cholera virus grown in PK-15 cells and SK-cells was labeled with [35S] methionine and [3H] uridine. At least 3 polypeptides were resolved by polyacrylamide gel electro­ phoresis after disruption of the virus with sodium dodecyl-sulfate. The molecular weights of the structural proteins were determined to be 55000 (p55), 46000 (gp46), and 36000 (p36). The molecular weight of the viral RNA was determined to be about 4 X 106 in polyacrylamide-agarose-gel electrophoresis. In sucrose gradients the RNA has a S20,w value of 40 -45S.

Luminography – an Alternative Assay for Detection of von Willebrand Factor Multimers

1988 ◽  
Vol 60 (02) ◽  
pp. 133-136 ◽  
Author(s):  
R Schneppenheim ◽  
H Plendl ◽  
U Budde
Keyword(s):  
Von Willebrand Factor ◽  
Gel Electrophoresis ◽  
Detection Methods ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Luminescence Assay ◽  
Von Willebrand ◽  
Willebrand Factor

SummaryA luminescence assay was adapted for detection of von Willebrand factor multimers subsequent to SDS-agarose gel electrophoresis and electroblotting onto nitrocellulose. The method is as fast as chromogenic detection methods and appears to be as sensitive as autoradiography without the disadvantages of the latter.

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