Adenosine cyclic 3',5'-monophosphate dependent protein kinase: a new fluorescence displacement titration technique for characterizing the nucleotide binding site on the catalytic subunit

Biochemistry ◽  
1983 ◽  
Vol 22 (26) ◽  
pp. 6310-6317 ◽  
Author(s):  
Deepak Bhatnagar ◽  
Robert Roskoski ◽  
Mary S. Rosendahl ◽  
Nelson J. Leonard
Keyword(s):  
Protein Kinase ◽  
Binding Site ◽  
Protein Kinase A ◽  
Nucleotide Binding ◽  
Nucleotide Binding Site ◽  
Catalytic Subunit ◽  
Dependent Protein Kinase ◽  
Dependent Protein ◽  
Titration Technique ◽  
Kinase A

scholarly journals Inactivation of the Catalytic Subunit of cAMP-Dependent Protein Kinase A Causes Delayed Appressorium Formation and Reduced Pathogenicity ofColletotrichum gloeosporioides

2012 ◽  
Vol 2012 ◽  
pp. 1-12 ◽  
Author(s):  
Tri Puji Priyatno ◽  
Farah Diba Abu Bakar ◽  
Nurhaida Kamaruddin ◽  
Nor Muhammad Mahadi ◽  
Abdul Munir Abdul Murad
Keyword(s):  
Protein Kinase ◽  
Protein Kinase A ◽  
Pathogenic Fungus ◽  
Pathogenic Fungi ◽  
Catalytic Subunit ◽  
Pathogenicity Test ◽  
Dependent Protein Kinase ◽  
Camp Dependent Protein Kinase ◽  
Dependent Protein ◽  
Kinase A

The cyclic AMP- (cAMP-) dependent protein kinase A signaling pathway is one of the major signaling pathways responsible for regulation of the morphogenesis and pathogenesis of several pathogenic fungi. To evaluate the role of this pathway in the plant pathogenic fungus,Colletotrichum gloeosporioides, the gene encoding the catalytic subunit of cAMP-dependent protein kinase A,CgPKAC, was cloned, inactivated, and the mutant was analyzed. Analysis of theCgpkacmutant generated via gene replacement showed that the mutants were able to form appressoria; however, their formation was delayed compared to the wild type. In addition, the mutant conidia underwent bipolar germination after appressoria formation, but no appressoria were generated from the second germ tube. The mutants also showed reduced ability to adhere to a hydrophobic surface and to degrade lipids localized in the appressoria. Based on the number of lesions produced during a pathogenicity test, the mutant’s ability to cause disease in healthy mango fruits was reduced, which may be due to failure to penetrate into the fruit. These findings indicate that cAMP-dependent protein kinase A has an important role in regulating morphogenesis and is required for pathogenicity ofC. gloeosporioides.

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