Photoaffinity labeling of the sodium- and potassium-activated adenosinetriphosphatase with a cardiac glycoside containing the photoactive group on the C-17 side chain

Biochemistry ◽  
1983 ◽  
Vol 22 (26) ◽  
pp. 6303-6309 ◽  
Author(s):  
Tamboue Deffo ◽  
Dwight S. Fullerton ◽  
Masaru Kihara ◽  
Reginald H. McParland ◽  
Robert R. Becker ◽  
...  
Keyword(s):  
Cardiac Glycoside ◽  
Photoaffinity Labeling ◽  
Side Chain ◽  
Sodium And Potassium

scholarly journals Resolution of Pump and Leak Components of Sodium and Potassium Ion Transport in Human Erythrocytes

1967 ◽  
Vol 50 (5) ◽  
pp. 1201-1220 ◽  
Author(s):  
R. L. Post ◽  
C. D. Albright ◽  
K. Dayani
Keyword(s):  
Cardiac Glycoside ◽  
Potassium Ion ◽  
Sodium Ion ◽  
The Other ◽  
Potassium Ions ◽  
Sodium Ions ◽  
Passive Transport ◽  
Potassium Ion Transport ◽  
Pump Activity ◽  
Sodium And Potassium

Further support for the pump-leak concept was obtained. Net transport was resolved into pump and leak components with the cardiac glycoside, ouabain. The specificity of ouabain as a pump inhibitor was demonstrated by its ineffectiveness when the pump was already inhibited by lack of one of the three pump substrates, sodium ion, potassium ion, or adenosine triphosphate. In the presence of ouabain the rates of passive transport of sodium and potassium ions changed almost in proportion to changes in their extracellular concentrations when one ion was exchanged for the other. In the presence of ouabain and at the extracellular concentrations which produced zero net transport, the ratio of potassium ions to sodium ions was 1.2-fold higher inside the cells than outside. This finding was attributed to a residual pump activity of less than 2% of capacity. The permeability to potassium ions was 10% greater than the permeability to sodium ions. A test was made of the independence of pump and leak. Conditions were chosen to change the rate through each pathway separately or in combination. When both pathways were active, net transport was the sum of the rates observed when each acted separately. A ratio of three sodium ions pumped outward per two potassium ions pumped inward was confirmed.

Fluid secretion by the malpighian tubules of the tsetse fly Glossina morsitans: the effects of ouabain, ethacrynic acid and amiloride

1976 ◽  
Vol 65 (2) ◽  
pp. 323-332
Author(s):  
J. D. Gee
Keyword(s):  
Sodium Transport ◽  
Cardiac Glycoside ◽  
Ethacrynic Acid ◽  
Fluid Secretion ◽  
Sodium Concentration ◽  
Tsetse Fly ◽  
Malpighian Tubules ◽  
Sodium Ions ◽  
Glossina Morsitans ◽  
Sodium And Potassium

The effects of three inhibitors of sodium transport on the secretion of fluid by the Malpighian tubules of Glossina morsitans have been observed. The cardiac glycoside, ouabain, affects neither the rate of secretion nor the sodium concentration of the fluid secreted when isolated tubules are bathed by solutions containing a range of sodium and potassium concentrations. Secretion is inhibited, however, by ethacrynic acid and amiloride. The results confirm that fluid secretion by the Malpighian tubules of this insect is dependent on the active transport of sodium ions and show that Na+/k+ exchange pumps are not involved in this process.

Polyether carboxylic acids incorporating a benzodioxinic subunit: synthesis and complexing ability of sodium and potassium

1992 ◽  
Vol 70 (3) ◽  
pp. 828-835 ◽  
Author(s):  
Pascal Bosseray ◽  
Gérard Coudert ◽  
Gérald Guillaumet ◽  
Georges Jeminet ◽  
Madeleine Tissier ◽  
...  
Keyword(s):  
Carboxylic Acids ◽  
Formation Constants ◽  
Side Chain ◽  
Complexing Ability ◽  
Potentiometric Measurements ◽  
Sodium And Potassium

We report here the synthesis of new polyoxygenated macrocyclic derivatives with a benzodioxinic subunit and a carboxylic side chain (2, 3), along with some of their corresponding acyclic polyethers (1). Formation constants of sodium and potassium complexes with both the acid and anion forms of these compounds were obtained from potentiometric measurements. The enhancement of both complexing ability and selectivity afforded by the presence of a carboxylic side chain is discussed. Keywords: polyether carboxylic acids, macrocycles, benzo-1,4-dioxin, sodium and potassium complexes, solvent methanol.

Specific photoaffinity labeling of the digitalis binding site of the sodium and potassium ion-activated adenosine triphosphatase induced by energy transfer

Biochemistry ◽  
1983 ◽  
Vol 22 (20) ◽  
pp. 4685-4690 ◽  
Author(s):  
Maurice P. Goeldner ◽  
Christian G. Hirth ◽  
Bernard Rossi ◽  
Gilles Ponzio ◽  
Michel Lazdunski
Keyword(s):  
Energy Transfer ◽  
Binding Site ◽  
Adenosine Triphosphatase ◽  
Photoaffinity Labeling ◽  
Potassium Ion ◽  
Sodium And Potassium
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