.beta.-Hydroxydecanoyl thioester dehydrase does not catalyze a rate-limiting step in Escherichia coli unsaturated fatty acid synthesis

Biochemistry ◽  
1983 ◽  
Vol 22 (25) ◽  
pp. 5897-5902 ◽  
Author(s):  
David P. Clark ◽  
Diego DeMendoza ◽  
Mary L. Polacco ◽  
John E. Cronan
Keyword(s):  
Escherichia Coli ◽  
Fatty Acid ◽  
Fatty Acid Synthesis ◽  
Unsaturated Fatty Acid ◽  
Acid Synthesis ◽  
Rate Limiting Step ◽  
Limiting Step ◽  
Rate Limiting

Fatty acid uptake in Escherichia coli: regulation by recruitment of fatty acyl-CoA synthetase to the plasma membrane

1993 ◽  
Vol 71 (1-2) ◽  
pp. 51-56 ◽  
Author(s):  
Dev Mangroo ◽  
Gerhard E. Gerber
Keyword(s):  
Escherichia Coli ◽  
Plasma Membrane ◽  
Fatty Acid ◽  
Fatty Acid Uptake ◽  
Fatty Acyl ◽  
Acid Uptake ◽  
Bacterial Membranes ◽  
Rate Limiting Step ◽  
Triton X ◽  
Rate Limiting

Fatty acid uptake in Escherichia coli has been shown to be inhibited by starvation and to be reversed by a short preincubation of the starved cells with D- or L-lactate, succinate, and acetate; these effects on oleate uptake were due to regulation of the rate-limiting step which involves fatty acyl-CoA synthetase. Investigation into the mechanism of regulation of fatty acyl-CoA synthetase showed that D-lactate did not affect the activity of the enzyme directly. Fatty acyl-CoA synthetase was found to be activated by about 20-fold by Triton X-100 and by another 4-fold by the addition of bacterial membranes. D-Lactate treatment was shown to result in coisolation of fatty acyl-CoA synthetase with the plasma membrane; these results are consistent with the interpretation that recruitment of the enzyme to the plasma membrane by D-lactate results in its activation and consequently in the increased level of fatty acid uptake.Key words: fatty acid, uptake, regulation, recruitment, fatty acyl-CoA synthetase, Escherichia coli, plasma membrane.

Sign in / Sign up

Export Citation Format

Share Document