Conformational Equilibrium of the Reactive Center Loop of Antithrombin Examined by Steady State and Time-Resolved Fluorescence Measurements:  Consequences for the Mechanism of Factor Xa Inhibition by Antithrombin−Heparin Complexes†

Biochemistry ◽  
2001 ◽  
Vol 40 (22) ◽  
pp. 6680-6687 ◽  
Author(s):  
Akiko Futamura ◽  
Joseph M. Beechem ◽  
Peter G. W. Gettins
Keyword(s):  
Steady State ◽  
Conformational Equilibrium ◽  
Factor Xa ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Reactive Center Loop ◽  
Fluorescence Measurements ◽  
Reactive Center ◽  
Factor Xa Inhibition ◽  
Heparin Complexes

Concentration and Temperature Dependence of Laurdan Fluorescence in Glycerol

2003 ◽  
Vol 58 (9-10) ◽  
pp. 581-588 ◽  
Author(s):  
K. A. Kozyra ◽  
J. R. Heldt ◽  
J. Heldt ◽  
M. Engelkec ◽  
H. A. Diehl
Keyword(s):  
Steady State ◽  
Time Distribution ◽  
Emission Spectra ◽  
Fluorescence Decay ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Fluorescence Measurements ◽  
Fluorescence Decay Time ◽  
Laurdan Fluorescence ◽  
Vibrational Equilibrium

Steady-state and time-resolved fluorescence measurements have been performed on Laurdan, dissolved in viscous glycerol, as functions of temperature and concentration. The results indicate spectral heterogeneity of the Laurdan solution. The fluorescence decay time distribution is attributed to radiative deexcitation of spatial conformational forms of locally excited (LE) and charge transfer (CT) states, the S1(CT)EQ state being in thermodynamic and vibrational equilibrium. The lifetimes and contributions of the different fluorescence modes depend on concentration and temperature. The excitation and emission spectra show discontinuous changes with increase of the Laurdan concentration.We suppose that the observed changes are caused by the formation of Laurdan micelle aggregates.

scholarly journals ANG-2 for quantitative Na+determination in living cells by time-resolved fluorescence microscopy

2014 ◽  
Vol 13 (12) ◽  
pp. 1699-1710 ◽  
Author(s):  
Phillip Roder ◽  
Carsten Hille
Keyword(s):  
Steady State ◽  
Fluorescence Microscopy ◽  
Living Cells ◽  
Fluorescent Dye ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Fluorescence Measurements

A new Na+-sensitive fluorescent dye ANG-2 was characterised photophysically (1P- and 2P-absorption, steady-state and time-resolved fluorescence) and its usability for quantitative Na+recordings in living cells by time-resolved fluorescence measurements was demonstrated.

A Spectroscopic Approach with Theoretical Studies to Study the Interaction of 9-aminoacridine with Certain Phenols

2017 ◽  
Vol 231 (5) ◽  
Author(s):  
Chandrakumar Manivannan ◽  
Rajadurai Vijay Solomon ◽  
Ponnambalam Venuvanalingam ◽  
Rajalingam Renganathan
Keyword(s):  
Steady State ◽  
Bathochromic Shift ◽  
Acetonitrile Solution ◽  
Theoretical Studies ◽  
Time Resolved Fluorescence ◽  
Quenching Rate ◽  
Time Resolved ◽  
Fluorescence Measurements ◽  
Quenching Rate Constant ◽  
Bimolecular Quenching

AbstractThe influence of phenols upon the fluorescence quenching of 9-aminoacridine (9-AA) was examined in acetonitrile solution by employing steady state and time-resolved fluorescence measurements. On increasing the concentration of quencher molecules the absorption spectra of 9-AA change with significant bathochromic shift. The fluorescence intensity of 9-AA change in presence of quencher molecules were measured at various temperatures as a function of the quencher concentrations. The observed bimolecular quenching rate constant (k

Steady-state and time-resolved fluorescence measurements for studying molecular interactions: interaction of a calcium-binding probe with proteins

1996 ◽  
Vol 62 (1-3) ◽  
pp. 25-38 ◽  
Author(s):  
Kim Marie Hirshfield ◽  
Dmitri Toptygin ◽  
Gopal Grandhige ◽  
Hans Kim ◽  
Beverly Z. Packard ◽  
...  
Keyword(s):  
Steady State ◽  
Molecular Interactions ◽  
Calcium Binding ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Fluorescence Measurements

Study between solvatochromism and steady-state and time-resolved fluorescence measurements of the Methylene blue in binary mixtures

2015 ◽  
Vol 119 ◽  
pp. 12-21 ◽  
Author(s):  
Luiza R.R. Santin ◽  
Sandra C. dos Santos ◽  
Diogo La Rosa Novo ◽  
Daniela Bianchini ◽  
Adriana P. Gerola ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Steady State ◽  
Binary Mixtures ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Fluorescence Measurements
Sign in / Sign up

Export Citation Format

Share Document