Fluorine-19 nuclear magnetic resonance studies of lipid fatty acyl chain order and dynamics in Acholeplasma laidlawii B membranes. A physical, biochemical, and biological evaluation of monofluoropalmitic acids as membrane probes

Biochemistry ◽  
1983 ◽  
Vol 22 (22) ◽  
pp. 5097-5103 ◽  
Author(s):  
Brian McDonough ◽  
Peter M. Macdonald ◽  
Brian D. Sykes ◽  
Ronald N. McElhaney
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Acyl Chain ◽  
Biological Evaluation ◽  
Fatty Acyl ◽  
Fatty Acyl Chain ◽  
Magnetic Resonance Studies ◽  
Acholeplasma Laidlawii ◽  
Chain Order ◽  
Membrane Probes

Fatty acyl chain order in lecithin model membranes determined from proton magnetic resonance

Biochemistry ◽  
1978 ◽  
Vol 17 (26) ◽  
pp. 5750-5762 ◽  
Author(s):  
Myer Bloom ◽  
E. Elliott Burnell ◽  
Alexander L. MacKay ◽  
Christine P. Nichol ◽  
Marko I. Valic ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Proton Magnetic Resonance ◽  
Acyl Chain ◽  
Fatty Acyl ◽  
Model Membranes ◽  
Fatty Acyl Chain ◽  
Chain Order

Fatty acyl chain structure, orientational order, and the lipid phase transition in Acholeplasma laidlawii B membranes. A review of recent 19F nuclear magnetic resonance studies

1984 ◽  
Vol 62 (11) ◽  
pp. 1134-1150 ◽  
Author(s):  
P. M. Macdonald ◽  
B. D. Sykes ◽  
R. N. McElhaney
Keyword(s):  
Phase Transition ◽  
Fatty Acids ◽  
Nuclear Magnetic Resonance ◽  
Acyl Chain ◽  
Orientational Order ◽  
Membrane Lipid ◽  
Fatty Acyl ◽  
Fatty Acyl Chain ◽  
Lipid Phase ◽  
Lipid Phase Transition

The orientational order parameters of monofluoropalmitic acids biosynthetically incorporated into membranes of Acholeplasma laidlawii B in the presence of a large excess of a variety of structurally diverse fatty acids have been determined via 19F nuclear magnetic resonance (19F NMR) spectroscopy. It is demonstrated that these monofluoropalmitic acids are relatively nonperturbing membrane probes based upon physical (differential scanning calorimetry), biochemical (membrane lipid analysis), and biological (growth studies) criteria. 19F NMR is shown to convey the same qualitative and quantitative picture of membrane lipid order provided by 2H-NMR techniques and to be sensitive to the structural characteristics of the membrane fatty acyl chains, as well as to the lipid phase transition. Representatives of each naturally occurring class of fatty acyl chain structures, including straight-chain saturated, methyl-branched, monounsaturated, and alicyclic-ring-substituted fatty acids, were studied and the 19F-NMR order parameters were correlated with the lipid phase transitions (determined calorimetrically). The lipid phase transition was the prime determinant of overall orientational order regardless of fatty acid structure. Effects upon orientational order attributable to specific structural substituents were discernible, but were secondary to the effects of the lipid phase transition. In the gel state, relative overall order was directly proportional to the temperature of the particular lipid phase transition. Not only the overall order, but also the order profile across the membrane was sensitive to the presence of particular structural substituents. In particular, in the gel state specific fatty acyl structures demonstrated a characteristic disordering effect in the membrane order profile. These various observations can be merged to provide a unified picture of the manner in which fatty acyl chain chemistry modulates the physical state of membrane lipids.

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