Disassembly and characterization of the nuclear pore complex lamina fraction from bovine liver

Biochemistry ◽  
1983 ◽  
Vol 22 (12) ◽  
pp. 2852-2860 ◽  
Author(s):  
Pamela A. Havre ◽  
David R. Evans
Keyword(s):  
Nuclear Pore Complex ◽  
Bovine Liver ◽  
Nuclear Pore

scholarly journals Stoichiometry and compositional plasticity of the yeast nuclear pore complex revealed by quantitative fluorescence microscopy

2018 ◽  
Vol 115 (17) ◽  
pp. E3969-E3977 ◽  
Author(s):  
Sasikumar Rajoo ◽  
Pascal Vallotton ◽  
Evgeny Onischenko ◽  
Karsten Weis
Keyword(s):  
Nuclear Pore Complex ◽  
Yeast Cells ◽  
Nuclear Pore ◽  
Altered Expression ◽  
Copy Numbers ◽  
Quantitative Image ◽  
Multiple Copies ◽  
Almost All ◽  
High Degree

The nuclear pore complex (NPC) is an eightfold symmetrical channel providing selective transport of biomolecules across the nuclear envelope. Each NPC consists of ∼30 different nuclear pore proteins (Nups) all present in multiple copies per NPC. Significant progress has recently been made in the characterization of the vertebrate NPC structure. However, because of the estimated size differences between the vertebrate and yeast NPC, it has been unclear whether the NPC architecture is conserved between species. Here, we have developed a quantitative image analysis pipeline, termed nuclear rim intensity measurement (NuRIM), to precisely determine copy numbers for almost all Nups within native NPCs of budding yeast cells. Our analysis demonstrates that the majority of yeast Nups are present at most in 16 copies per NPC. This reveals a dramatic difference to the stoichiometry determined for the human NPC, suggesting that despite a high degree of individual Nup conservation, the yeast and human NPC architecture is significantly different. Furthermore, using NuRIM, we examined the effects of mutations on NPC stoichiometry. We demonstrate for two paralog pairs of key scaffold Nups, Nup170/Nup157 and Nup192/Nup188, that their altered expression leads to significant changes in the NPC stoichiometry inducing either voids in the NPC structure or substitution of one paralog by the other. Thus, our results not only provide accurate stoichiometry information for the intact yeast NPC but also reveal an intriguing compositional plasticity of the NPC architecture, which may explain how differences in NPC composition could arise in the course of evolution.

scholarly journals Identification and Characterization of Nuclear Pore Complex Components in Arabidopsis thaliana

2010 ◽  
Vol 22 (12) ◽  
pp. 4084-4097 ◽  
Author(s):  
Kentaro Tamura ◽  
Yoichiro Fukao ◽  
Masaaki Iwamoto ◽  
Tokuko Haraguchi ◽  
Ikuko Hara-Nishimura
Keyword(s):  
Arabidopsis Thaliana ◽  
Nuclear Pore Complex ◽  
Nuclear Pore ◽  
Identification And Characterization ◽  
Complex Components

scholarly journals Characterization of the membrane-coating Nup84 complex: Paradigm for the nuclear pore complex structure

Nucleus ◽  
2010 ◽  
Vol 1 (2) ◽  
pp. 150-157 ◽  
Author(s):  
Erik W. Debler ◽  
Kuo-Chiang Hsia ◽  
Vivien Nagy ◽  
Hyuk-Soo Seo ◽  
André Hoelz
Keyword(s):  
Nuclear Pore Complex ◽  
Complex Structure ◽  
Nuclear Pore ◽  
Membrane Coating

scholarly journals Characterization of nuclear pore complex components in fission yeastSchizosaccharomyces pombe

Nucleus ◽  
2014 ◽  
Vol 5 (2) ◽  
pp. 149-162 ◽  
Author(s):  
Haruhiko Asakawa ◽  
Hui-Ju Yang ◽  
Takaharu G Yamamoto ◽  
Chizuru Ohtsuki ◽  
Yuji Chikashige ◽  
...  
Keyword(s):  
Nuclear Pore Complex ◽  
Nuclear Pore ◽  
Complex Components

scholarly journals Characterization of the Selective Barrier in Nuclear Pore Complex

2014 ◽  
Vol 106 (2) ◽  
pp. 313a
Author(s):  
Jiong Ma ◽  
Alexander Goryaynov ◽  
Weidong Yang
Keyword(s):  
Nuclear Pore Complex ◽  
Nuclear Pore

scholarly journals Characterization of a nuclear pore protein sheds light on the roles and composition of the Toxoplasma gondii nuclear pore complex

2017 ◽  
Vol 74 (11) ◽  
pp. 2107-2125 ◽  
Author(s):  
Flavie Courjol ◽  
Thomas Mouveaux ◽  
Kevin Lesage ◽  
Jean-Michel Saliou ◽  
Elisabeth Werkmeister ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Nuclear Pore Complex ◽  
Nuclear Pore ◽  
Nuclear Pore Protein ◽  
Pore Protein
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