Photochemical crosslinking of translation initiation factor-3 to Escherichia coli 50S ribosomal subunits

Biochemistry ◽  
1983 ◽  
Vol 22 (6) ◽  
pp. 1483-1489 ◽  
Author(s):  
Ira Schwartz ◽  
Mark Vincent ◽  
William A. Strycharz ◽  
Lawrence Kahan
2006 ◽  
Vol 51 (1) ◽  
pp. 175-180 ◽  
Author(s):  
Go Hirokawa ◽  
Hideko Kaji ◽  
Akira Kaji

ABSTRACT The effect of paromomycin on the interaction of ribosomal subunits was studied. Paromomycin inhibited the antiassociation activity of initiation factor 3 (IF3). Furthermore, ribosomal subunits were associated to form 70S ribosomes by paromomycin even in the presence of 1 mM Mg2+. Paromomycin did not inhibit the binding of IF3 to the 30S ribosomal subunits. On the other hand, IF3 bound to the 30S subunits was expelled by paromomycin-induced subunit association (70S formation). These results indicate that the stabilization of 70S ribosomes by paromomycin may in part be responsible for its inhibitory effects on translocation and ribosome recycling.


2014 ◽  
Vol 34 (6) ◽  
pp. 1046-1053 ◽  
Author(s):  
Y. Martineau ◽  
X. Wang ◽  
T. Alain ◽  
E. Petroulakis ◽  
D. Shahbazian ◽  
...  

2020 ◽  
Vol 14 (1) ◽  
pp. 93-97
Author(s):  
Libo Li ◽  
Stephanie O. Palmer ◽  
Elizabeth A. Gomez ◽  
Frank Mendiola ◽  
Tianzhi Wang ◽  
...  

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