Effect of Escherichia coli initiation factors on the kinetics of N-AcPhe-tRNAPhe binding to 30S ribosomal subunits. A fluorescence stopped-flow study

Biochemistry ◽  
1983 ◽  
Vol 22 (3) ◽  
pp. 690-694 ◽  
Author(s):  
Wolfgang Wintermeyer ◽  
Claudio Gualerzi
Keyword(s):  
Escherichia Coli ◽  
Stopped Flow ◽  
Ribosomal Subunits ◽  
Initiation Factors ◽  
Kinetics Of ◽  
Flow Study

scholarly journals Leaderless mRNAs Bind 70S Ribosomes More Strongly than 30S Ribosomal Subunits in Escherichia coli

2002 ◽  
Vol 184 (23) ◽  
pp. 6730-6733 ◽  
Author(s):  
Sean M. O'Donnell ◽  
Gary R. Janssen
Keyword(s):  
Escherichia Coli ◽  
Translation Initiation ◽  
Primer Extension ◽  
Ribosomal Subunits ◽  
Ribosome Binding ◽  
Initiation Factors ◽  
Translation Initiation Factors

ABSTRACT By primer extension inhibition assays, 70S ribosomes bound with higher affinity, or stability, than did 30S subunits to leaderless mRNAs containing AUG or GUG start codons. Addition of translation initiation factors affected ribosome binding to leaderless mRNAs. Our results suggest that translation of leaderless mRNAs might initiate through a pathway involving 70S ribosomes or 30S subunits lacking IF3.

Kinetics of the reversible association of ribosomal subunits: Stopped-flow studies of the rate law and of the effect of Mg2+

1975 ◽  
Vol 93 (4) ◽  
pp. 499-515 ◽  
Author(s):  
A. Wishnia ◽  
A. Boussert ◽  
M. Graffe ◽  
Ph. Dessen ◽  
M. Grunberg-Manago
Keyword(s):  
Stopped Flow ◽  
Ribosomal Subunits ◽  
Rate Law ◽  
Reversible Association ◽  
Kinetics Of

scholarly journals Assembly Kinetics of Vimentin Tetramers to Unit-Length Filaments: A Stopped-Flow Study

2018 ◽  
Vol 114 (10) ◽  
pp. 2408-2418 ◽  
Author(s):  
Norbert Mücke ◽  
Lara Kämmerer ◽  
Stefan Winheim ◽  
Robert Kirmse ◽  
Jan Krieger ◽  
...  
Keyword(s):  
Unit Length ◽  
Stopped Flow ◽  
Assembly Kinetics ◽  
Kinetics Of ◽  
Flow Study

Kinetics of "extraction" of copper(II) by micelle-solubilized complexing agents of varying hydrophilic lipophilic balance. 1. Stopped-flow study

1992 ◽  
Vol 96 (26) ◽  
pp. 11072-11078 ◽  
Author(s):  
Sung Geun Son ◽  
Marc Hebrant ◽  
Paolo Tecilla ◽  
Paolo Scrimin ◽  
Christian Tondre
Keyword(s):  
Complexing Agents ◽  
Stopped Flow ◽  
Hydrophilic Lipophilic Balance ◽  
Kinetics Of ◽  
Flow Study
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