Solution Structure of the Reps1 EH Domain and Characterization of Its Binding to NPF Target Sequences†,‡

Biochemistry ◽  
2001 ◽  
Vol 40 (23) ◽  
pp. 6776-6785 ◽  
Author(s):  
Soyoun Kim ◽  
Donald N. Cullis ◽  
Larry A. Feig ◽  
James D. Baleja
Keyword(s):  
Solution Structure ◽  
Eh Domain ◽  
Target Sequences

scholarly journals Characterization of the Solution Structure of Human Serum Albumin Loaded with a Metal Porphyrin and Fatty Acids

2011 ◽  
Vol 100 (9) ◽  
pp. 2293-2301 ◽  
Author(s):  
Matthias J.N. Junk ◽  
Hans W. Spiess ◽  
Dariush Hinderberger
Keyword(s):  
Fatty Acids ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Solution Structure ◽  
Metal Porphyrin

scholarly journals Solution Structure of Prosurvival Mcl-1 and Characterization of Its Binding by Proapoptotic BH3-only Ligands

2004 ◽  
Vol 280 (6) ◽  
pp. 4738-4744 ◽  
Author(s):  
Catherine L. Day ◽  
Lin Chen ◽  
Sarah J. Richardson ◽  
Penny J. Harrison ◽  
David C. S. Huang ◽  
...  
Keyword(s):  
Solution Structure

Solution Structure of Eps15's Third EH Domain Reveals Coincident Phe−Trp and Asn−Pro−Phe Binding Sites†,‡

Biochemistry ◽  
2000 ◽  
Vol 39 (15) ◽  
pp. 4309-4319 ◽  
Author(s):  
Jennifer L. Enmon ◽  
Tonny de Beer ◽  
Michael Overduin
Keyword(s):  
Binding Sites ◽  
Solution Structure ◽  
Eh Domain

scholarly journals Characterization of the solution structure of a neuroligin/β-neurexin complex

2008 ◽  
Vol 175 (1-3) ◽  
pp. 150-155 ◽  
Author(s):  
Davide Comoletti ◽  
Alexander Grishaev ◽  
Andrew E. Whitten ◽  
Palmer Taylor ◽  
Jill Trewhella
Keyword(s):  
Solution Structure

scholarly journals From Crystallographic Data to Solution Structure of Photoreceptors: The Case of AppA BLUF Domain

2021 ◽  
Author(s):  
Shaima Hashem ◽  
Veronica Macaluso ◽  
Michele Nottoli ◽  
Filippo Lipparini ◽  
Lorenzo Cupellini ◽  
...  
Keyword(s):  
Solution Structure ◽  
Molecular Mechanisms ◽  
Md Simulations ◽  
Hydrogen Bond Network ◽  
Light Activation ◽  
Activated State ◽  
Bond Network ◽  
Bluf Domain ◽  
First Time

<div>Photoreceptor proteins bind a chromophore, which, upon light absorption, modifies its geometry or its interactions with the protein, finally inducing the structural change needed to switch the protein from an inactive to an active or signaling state. In the Blue Light-Using Flavin (BLUF) family of photoreceptors, the chromophore is a flavin and the changes have been connected with a rearrangement of the hydrogen bond network around it on the basis of spectroscopic changes measured for the dark-to-light conversion. However, the exact conformational change triggered by the photoexcitation is still elusive mainly because a clear consensus on the identity not only of the light activated state but also of the dark one has not been achieved. Here, for the first time, we present an integrated investigation that combines microsecond MD simulations starting from the two conflicting crystal structures available for the AppA BLUF domain with calculations of NMR, IR and UV-Vis spectra using a polarizable QM/MM approach. Thanks to such a combined analysis of the three different spectroscopic responses, a robust characterization of the structure of the dark state in solution is given together with the uncovering of important flaws of the most popular molecular mechanisms present in the literature for the dark-to-light activation. </div>

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