A homozygous human cell line contains three subsets of HLA-DR-like antigens distinguishable by amino acid sequencing

Biochemistry ◽  
1982 ◽  
Vol 21 (26) ◽  
pp. 6625-6628 ◽  
Author(s):  
David W. Andrews ◽  
Jack L. Strominger ◽  
M. Rosa Bono
Keyword(s):  
Amino Acid ◽  
Cell Line ◽  
Human Cell ◽  
Human Cell Line ◽  
Amino Acid Sequencing ◽  
Hla Dr

scholarly journals Epigenetic Modifier Mutations in the LL-100 Panel

Blood ◽  
2018 ◽  
Vol 132 (Supplement 1) ◽  
pp. 5271-5271
Author(s):  
Hilmar Quentmeier ◽  
Claudia Pommerenke ◽  
Hans G. Drexler
Keyword(s):  
Amino Acid ◽  
Drug Development ◽  
Cell Line ◽  
Cell Lines ◽  
Human Cell ◽  
Conflicts Of Interest ◽  
Human Cancer ◽  
Human Cell Line ◽  
Dominant Negative ◽  
Epigenetic Modifier

Abstract The NCI-60 human cell line panel, developed for use in drug development comprises sixty human cancer cell lines derived from nine different tissues. Only six cell lines of the NCI-60 were derived from blood cancers. Therefore, most forms and subtypes of leukemia and lymphoma are not represented in the NCI-60 panel. To respond to this apparent gap, we suggest the novel LL-100 panel, 100 leukemia and lymphoma cell lines representing the major leukemia/lymphoma entities, for basic research and drug development. Whole exome sequencing and RNA sequencing were performed to identify mutations in 100 cell lines. Here we list the 100 cell lines, ordered by subtype and show mutations in epigenetic modifier genes. We found cell lines with mutations in ASXL1, EZH2, IDH1, TET2 and in DNMT3A. Hitherto, cell line OCI-AML3 was the only human cell line described with a DNMT3A mutation. Twenty-two percent of patients with acute myeloid leukemia contain DNMT3A mutations and the median overall survival with DNMT3A mutations is shorter than without. Most DNMT3A mutations are heterozygous and alter amino acid R882, R882H being the most common DNMT3A mutation in AML. Exogenously mutant murine R878H (equivalent to human R882H) inhibits DNMT3A activity in a dominant negative manner. We describe here that the AML cell line SET-2 carries a heterozygous G to A transition at chr2_25234373 (hg38) which leads to the DNMT3A R882H amino acid substitution. Chip-based methylation analysis revealed that the described DNMT3A targets IRF8, KLF2, HOXA11 and HOXB2 are hypomethylated in cell lines OCI-AML3 (DNMT3A R882C) and in SET-2 (DNMT3A R882H). These data suggest that SET-2 is a novel model cell line for functional analysis of the DNMT3A R882 mutation and a first gain in knowledge through data mining the LL-100 panel. Disclosures No relevant conflicts of interest to declare.

scholarly journals Estrogen Receptor in a Human Cell Line (MCF-7) from Breast Carcinoma

1973 ◽  
Vol 248 (17) ◽  
pp. 6251-6253 ◽  
Author(s):  
Samuel C. Brooks ◽  
Elizabeth R. Locke ◽  
Herbert D. Soule
Keyword(s):  
Estrogen Receptor ◽  
Breast Carcinoma ◽  
Cell Line ◽  
Human Cell ◽  
Human Cell Line ◽  
Mcf 7

Local Regulation of Adrenal Steroidogenesis: Subtle in vitro Effects of IL-1β on the Human Cell Line NCI-H295R Steroid Production along with Changes in MicroRNA Profile and Orphan Nuclear Receptors NR4As

2021 ◽  
pp. 1-11
Author(s):  
Natalia Santucci ◽  
Rocío Stampone ◽  
Eduardo Brandão Ferreira da Silva ◽  
Silvina Villar ◽  
Silvana Spinelli ◽  
...  
Keyword(s):  
Adrenal Gland ◽  
Cell Line ◽  
Nuclear Receptors ◽  
Human Cell ◽  
Human Cell Line ◽  
Mirna Profile ◽  
Orphan Nuclear Receptors ◽  
Steroid Production ◽  
Adrenal Steroid ◽  
Adrenal Steroidogenesis

<b><i>Introduction:</i></b> IL-1β, a cytokine from the innate immune response, is well known for its proinflammatory effects and stimulating activity on the hypothalamus-pituitary-adrenal axis, leading to the pituitary synthesis of adrenocorticotropic hormone followed by cortisol (and dehydroepiandrosterone – DHEA) release by the adrenal gland. While IL-1β modulates the adrenal steroidogenesis at the central level, it is unclear whether it also exerts an effect on the adrenal gland. <b><i>Method:</i></b> We studied the effect of IL-1β on adrenal steroid production and steroidogenic enzyme RNA expression in the human cell line NCI-H295R. We also explored eventual changes in the microRNA (miRNA) profile from IL-1β-treated NCI-H295R cells. <b><i>Results:</i></b> Transcripts encoding IL-1β receptors 1 and 2 were noticeable in the cell line, with cortisol and DHEA production showing a subtle increase after cytokine treatment. Transcripts from key enzymes in the steroidogenic pathway were analyzed, with no noticeable changes on them. The miRNA profile was modified by IL-1β treatment to an extent which bears some relationship with the regulatory mechanisms underlying adrenal steroid production. Since orphan nuclear receptors NR4As have emerged as potential key factors for coordinating inflammatory and metabolic responses, cell expression studies were also carried out to show an NR4As transcript augmentation following IL-1β treatment. <b><i>Discussion/Conclusions:</i></b> The subtle increase in adrenal steroid production in response to IL-1β stimulation without any modification in the transcription of the steroidogenic enzymes analyzed suggests an additional inflammatory/anti-inflammatory loop, wherein NR4As receptors may participate. Besides its physiological role, this process might be implied in pathological states accompanied by an unbalanced immune-endocrine relationship.

Reduced cytotoxicity of tetracyclines to a multi-drug resistant human cell line

1988 ◽  
Vol 37 (15) ◽  
pp. 3038-3041 ◽  
Author(s):  
Maria Grandi ◽  
Fernando C. Giuliani
Keyword(s):  
Cell Line ◽  
Human Cell ◽  
Human Cell Line ◽  
Drug Resistant

scholarly journals Competition between Mg2+ and spermine for a cloned IRK2 channel expressed in a human cell line.

1996 ◽  
Vol 493 (1) ◽  
pp. 143-156 ◽  
Author(s):  
T Yamashita ◽  
Y Horio ◽  
M Yamada ◽  
N Takahashi ◽  
C Kondo ◽  
...  
Keyword(s):  
Cell Line ◽  
Human Cell ◽  
Human Cell Line

Preliminary discovery of novel markers for human cell line activation test (h-CLAT)

2021 ◽  
pp. 105154
Author(s):  
Aneesh V. Karkhanis ◽  
Eric Chun Yong Chan ◽  
Ee Chee Ren
Keyword(s):  
Cell Line ◽  
Human Cell ◽  
Human Cell Line ◽  
Activation Test
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