Deoxyribonucleic acid dependent adenosine triphosphatases from the Novikoff hepatoma. Characterization of a homogeneous adenosine triphosphatase that stimulates DNA polymerase .beta.

Biochemistry ◽  
1982 ◽  
Vol 21 (20) ◽  
pp. 5060-5068 ◽  
Author(s):  
David C. Thomas ◽  
Ralph R. Meyer
Keyword(s):  
Dna Polymerase ◽  
Adenosine Triphosphatase ◽  
Deoxyribonucleic Acid ◽  
Dna Polymerase Beta ◽  
Novikoff Hepatoma ◽  
Polymerase Beta ◽  
Adenosine Triphosphatases

scholarly journals Involvement of deoxyribonucleic acid polymerase β in nuclear deoxyribonucleic acid synthesis

1978 ◽  
Vol 173 (1) ◽  
pp. 309-314 ◽  
Author(s):  
T R Butt ◽  
W M Wood ◽  
E L McKay ◽  
R L P Adams
Keyword(s):  
Dna Synthesis ◽  
Dna Polymerase ◽  
Deoxyribonucleic Acid ◽  
Nuclear Dna ◽  
Dna Polymerase Beta ◽  
Cell Nuclei ◽  
High Molecular Weight Dna ◽  
Dna Polymerase Alpha ◽  
Polymerase Beta

The effects on DNA synthesis in vitro in mouse L929-cell nuclei of differential extraction of DNA polymerases alpha and beta were studied. Removal of all measurable DNA polymerase alpha and 20% of DNA polymerase beta leads to a 40% fall in the replicative DNA synthesis. Removal of 70% of DNA polymerase beta inhibits replicative synthesis by 80%. In all cases the nuclear DNA synthesis is sensitive to N-ethylmaleimide and aCTP (arabinosylcytosine triphosphate), though less so than DNA polymerase alpha. Addition of deoxyribonuclease I to the nuclear incubation leads to synthesis of high-molecular-weight DNA in a repair reaction. This occurs equally in nuclei from non-growing or S-phase cells. The former nuclei lack DNA polymerase alpha and the reaction reflects the sensitivity of DNA polymerase beta to inhibiton by N-ethylmaleimide and aCTP.

Expression of human DNA polymerase .beta. in Escherichia coli and characterization of the recombinant enzyme

Biochemistry ◽  
1988 ◽  
Vol 27 (3) ◽  
pp. 901-909 ◽  
Author(s):  
John Abbotts ◽  
Dibyendu N. SenGupta ◽  
Barbara Zmudzka ◽  
Steven G. Widen ◽  
Vicente Notario ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Dna Polymerase ◽  
Recombinant Enzyme ◽  
Dna Polymerase Beta ◽  
Polymerase Beta ◽  
Human Dna

scholarly journals Characterization of Messenger RNA for Chick-Embryo DNA Polymerase beta and Its Translation Product in vitro

1983 ◽  
Vol 133 (2) ◽  
pp. 277-282 ◽  
Author(s):  
Masamitsu YAMAGUCHI ◽  
Taijo TAKAHASHI ◽  
Kunio YASUDA ◽  
Yoshiro SHIMURA ◽  
Akio MATSUKAGE
Keyword(s):  
Chick Embryo ◽  
Dna Polymerase ◽  
Messenger Rna ◽  
Dna Polymerase Beta ◽  
Translation Product ◽  
Polymerase Beta

Mammalian DNA polymerase .beta.: Characterization of a 16-kDa transdomain fragment containing the nucleic acid-binding activities of the native enzyme

Biochemistry ◽  
1992 ◽  
Vol 31 (42) ◽  
pp. 10272-10280 ◽  
Author(s):  
Jose R. Casas-Finet ◽  
Amalendra Kumar ◽  
Richard L. Karpel ◽  
Samuel H. Wilson
Keyword(s):  
Nucleic Acid ◽  
Dna Polymerase ◽  
Dna Polymerase Beta ◽  
Native Enzyme ◽  
Nucleic Acid Binding ◽  
Polymerase Beta
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