Evidence on the existence of a purine ligand induced conformational change in the active site of bovine pancreatic ribonuclease A studied by proton nuclear magnetic resonance spectroscopy

Biochemistry ◽  
1982 ◽  
Vol 21 (18) ◽  
pp. 4290-4297 ◽  
Author(s):  
Carles Arus ◽  
Livio Paolillo ◽  
Rafel Llorens ◽  
Roberto Napolitano ◽  
Claudi M. Cuchillo
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Nuclear Magnetic Resonance Spectroscopy ◽  
Active Site ◽  
Ribonuclease A ◽  
Proton Nuclear Magnetic Resonance ◽  
Resonance Spectroscopy ◽  
Pancreatic Ribonuclease ◽  
Pancreatic Ribonuclease A

scholarly journals Sperm Metabolomics through Nuclear Magnetic Resonance Spectroscopy

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1669
Author(s):  
Marta Lombó ◽  
Sara Ruiz-Díaz ◽  
Alfonso Gutiérrez-Adán ◽  
María-Jesús Sánchez-Calabuig
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Nuclear Magnetic Resonance Spectroscopy ◽  
Current Knowledge ◽  
Proton Nuclear Magnetic Resonance ◽  
Farm Animals ◽  
Plasma Metabolites ◽  
Resonance Spectroscopy ◽  
Nuclear Magnetic

This report reviews current knowledge of sperm metabolomics analysis using proton nuclear magnetic resonance spectroscopy (1 H-NMR) with particular emphasis on human and farm animals. First, we present the benefits of NMR over other techniques to identify sperm metabolites and then describe the specific methodology required for NMR sperm analysis, stressing the importance of analyzing metabolites extracted from both the hydrophilic and lipophilic phases. This is followed by a description of advances produced to date in the use of NMR to diagnose infertility in humans and to identify metabolic differences among the sperm of mammalian herbivore, carnivore, and omnivore species. This last application of NMR mainly seeks to explore the possible use of lipids to fuel sperm physiology, contrary to previous theories that glycolysis and oxidative phosphorylation (OXPHOS) are the only sources of sperm energy. This review describes the use of NMR to identify sperm and seminal plasma metabolites as possible indicators of semen quality, and to examine the metabolites needed to maintain sperm motility, induce their capacitation, and consequently, to predict animal fertility.

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