Kinetic relationships between insulin receptor binding and effects on glucose transport in isolated rat adipocytes

Biochemistry ◽  
1982 ◽  
Vol 21 (14) ◽  
pp. 3475-3480 ◽  
Author(s):  
Theodore P. Ciaraldi ◽  
Jerrold M. Olefsky
Keyword(s):  
Insulin Receptor ◽  
Glucose Transport ◽  
Receptor Binding ◽  
Rat Adipocytes ◽  
Insulin Receptor Binding ◽  
Isolated Rat

Effect of acute zinc deficiency on insulin receptor binding in rat adipocytes

1992 ◽  
Vol 32 (1-3) ◽  
pp. 331-335 ◽  
Author(s):  
Marie Jeanne Gomot ◽  
Patrice Faure ◽  
Anne-Marie Roussel ◽  
Charles Coudray ◽  
Mireille Osman ◽  
...  
Keyword(s):  
Insulin Receptor ◽  
Zinc Deficiency ◽  
Receptor Binding ◽  
Rat Adipocytes ◽  
Insulin Receptor Binding

Insulin binding and action on fat cells from young healthy females and males

1982 ◽  
Vol 243 (2) ◽  
pp. E158-E167 ◽  
Author(s):  
O. Pedersen ◽  
E. Hjollund ◽  
H. O. Lindskov
Keyword(s):  
Glucose Metabolism ◽  
Cell Surface ◽  
Insulin Receptor ◽  
Glucose Transport ◽  
Receptor Binding ◽  
Glucose Utilization ◽  
Insulin Binding ◽  
Fat Cells ◽  
Insulin Receptor Binding ◽  
Rate Limiting

Insulin binding and action were studied in fat cells from the gluteal region of young healthy subjects. Fat cells from females were larger than those of males, had higher insulin receptor binding and higher rates of noninsulin-stimulated and maximally insulin-stimulated rates of methylglucose transport and glucose metabolism when these data were expressed per cell number. However, when insulin binding and insulin effects were expressed per cell surface, which may be physiologically more relevant, no sex differences were found in insulin binding and glucose transport, whereas noninsulin-stimulated and maximally insulin-stimulated glucose metabolism was still significantly increased in female fat cells. The latter indicates postreceptor differences in glucose metabolism between females and males. The insulin concentrations causing half-maximal responses (a measure of the sensitivity to insulin) of glucose transport, glucose metabolism and lipolysis were similar in fat cells from the two sexes, which is consistent with the comparable values of insulin receptor binding when adjusted to cell surface. Studies of rate-determining steps for the glucose utilization of human fat cells showed that glucose transport was not the rate-limiting step at physiological glucose concentrations. Moreover, at physiological glucose levels, glucose metabolism exhibited a decreased maximal insulin responsiveness and an increased insulin sensitivity when compared with glucose metabolism at low glucose concentrations at which glucose transport is rate limiting for the fat cell glucose utilization.

Effect of insulin-like factors on glucose transport activity in unweighted rat skeletal muscle

1993 ◽  
Vol 75 (2) ◽  
pp. 820-824 ◽  
Author(s):  
E. J. Henriksen ◽  
L. S. Ritter
Keyword(s):  
Skeletal Muscle ◽  
Insulin Receptor ◽  
Phospholipase C ◽  
Glucose Transport ◽  
Receptor Binding ◽  
Transport Activity ◽  
Insulin Effect ◽  
Insulin Receptor Binding ◽  
Igf I ◽  
Binding Mechanisms

We have previously demonstrated that mechanical unweighting of the soleus muscle by hindlimb suspension leads to increases in insulin receptor binding, muscle/fat-specific glucose transporter (GLUT-4) protein levels, and insulin-stimulated glucose transport activity. The present study used a novel approach to further evaluate the potential role of postreceptor binding mechanisms in this enhanced insulin effect after unweighting. Insulin-like growth factor I (IGF-I), vanadate, and phospholipase C were used to stimulate glucose transport activity independently of insulin receptor binding. Soleus glucose transport activity (assessed by 2-deoxyglucose uptake) was evaluated in vitro with soleus strips (approximately 18 mg). Progressively increased responses to maximally effective doses of insulin or IGF-I were observed after 3 and 6 days of unweighting compared with weight-matched control strips. Enhanced maximal responses to vanadate (6 days only) and phospholipase C (3 and 6 days) for 2-deoxyglucose uptake were also observed. The results of this study 1) provide evidence that post-insulin receptor binding mechanisms also play a role in the enhanced response of the insulin-dependent pathway for stimulation of glucose transport in unweighted skeletal muscle and 2) indicate that IGF-I action on glucose transport is included in this enhanced response in unweighted muscle.

scholarly journals Localization of the insulin receptor binding sites for the SH2 domain proteins p85, Syp, and GAP.

1994 ◽  
Vol 269 (44) ◽  
pp. 27186-27192
Author(s):  
P A Staubs ◽  
D R Reichart ◽  
A R Saltiel ◽  
K L Milarski ◽  
H Maegawa ◽  
...  
Keyword(s):  
Insulin Receptor ◽  
Receptor Binding ◽  
Binding Sites ◽  
Sh2 Domain ◽  
Insulin Receptor Binding
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