Phosphorylation Mutants Elucidate the Mechanism of Annexin IV-Mediated Membrane Aggregation†,‡

Biochemistry ◽  
2001 ◽  
Vol 40 (13) ◽  
pp. 4192-4199 ◽  
Author(s):  
M. A. Kaetzel ◽  
Y. D. Mo ◽  
T. R. Mealy ◽  
B. Campos ◽  
W. Bergsma-Schutter ◽  
...  
Keyword(s):  
Annexin Iv ◽  
Membrane Aggregation

Cellular and subcellular localization of annexin IV in rabbit intestinal epithelium, pancreas and liver

1991 ◽  
Vol 73 (2-3) ◽  
pp. 151-156 ◽  
Author(s):  
Dominique Massey ◽  
Valérie Traverso ◽  
Alain Rigal ◽  
Suzanne Maroux
Keyword(s):  
Subcellular Localization ◽  
Intestinal Epithelium ◽  
Annexin Iv

Annexin IV is Differentially Expressed in Clear Cell Carcinoma of the Ovary

2009 ◽  
Vol 19 (9) ◽  
pp. 1545-1549 ◽  
Author(s):  
Yi Miao ◽  
Bin Cai ◽  
Ling Liu ◽  
Yixia Yang ◽  
Xiaoping Wan
Keyword(s):  
Cell Carcinoma ◽  
Dna Microarray ◽  
Clear Cell ◽  
Clear Cell Carcinoma ◽  
Differentially Expressed ◽  
Serous Carcinoma ◽  
Chain Reaction ◽  
Complementary Dna ◽  
Polymerase Chain ◽  
Annexin Iv

Objective:To investigate the genes that were differentially expressed between clear cell carcinoma (CCC) and serous carcinoma (SAC) of the ovary with complementary DNA microarray.Methods:Complementary DNA microarray was carried out in 8 CCCs and 8 SACs of the ovary. Differentially expressed genes were identified and verified by real-time polymerase chain reaction. The expression of the protein was also verified with immunohistochemistry and Western blot in cells and tissues of ovarian CCC.Results:Comparison of the gene expression profiling identified 21 genes with more than 2-fold different expression between CCC and SAC of the ovary. The up-regulated and down-regulated genes were 9 and 12, respectively. The verification of Annexin IV in the cell line and tissues was in accordance with the result of the microarray.Conclusions:The complementary DNA microarray technique is a feasible way to explore the difference of the gene expression profiling between the 2 types of ovarian carcinoma. The overexpression of Annexin IV may be an ovarian CCC-specific molecular marker.Abbreviations:CCC- clear cell carcinoma, SAC- serous carcinoma, PCR- polymerase chain reaction, RT-PCR- reverse transcriptase PCR, ABCF2- ATP-binding cassette, sub-family F- member 2, HNF-1b- hepatocyte nuclear factor 1β

scholarly journals Different molecular arrangements of the tetrameric annexin 2 modulate the size and dynamics of membrane aggregation

2010 ◽  
Vol 1798 (9) ◽  
pp. 1790-1796 ◽  
Author(s):  
Françoise Illien ◽  
Stefanie Finet ◽  
Olivier Lambert ◽  
Jesus Ayala-Sanmartin
Keyword(s):  
Annexin 2 ◽  
Membrane Aggregation

scholarly journals In Vitro Study on the Regulation of Annexin IV and VEGF by hCG in the Human Endometrium

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Shaoyuan Xu ◽  
Jie Li ◽  
Xiaoyan Chen ◽  
Beiyu Liu
Keyword(s):  
Epithelial Cells ◽  
In Vitro Study ◽  
Control Group ◽  
Vascular Endothelial ◽  
Vegf Mrna ◽  
Endometrial Cells ◽  
Mrna And Protein Expression ◽  
Endothelial Growth Factor ◽  
Annexin Iv

Objective. Whether changes in vascular endothelial growth factor (VEGF) and annexin IV during implantation are regulated through the LH/hCG-R needs further research. To investigate the mechanism of hCG on the expression of annexin IV and VEGF in human endometrial cells. Methods. Endometrial cells were isolated and identified from human specimens. The proportion of glandular and epithelial cells was analyzed. Annexin IV and VEGF were analyzed by qRT-PCR (mRNA), western blot (proteins), and immunohistochemistry (proteins). Protein location was identified by immunohistochemistry. The cells were cultured with hCG, hCG/PD98059 (a MAPK inhibitor), or no treatment (control). Results. The proportions between the glandular epithelial cells and stromal cells at inoculation and when adding hCG were 25.8 ± 0.2% and 27.8 ± 0.04%, respectively ( P > 0.05 ). LH/hCG-R, annexin IV, and VEGF were found in the cytoplasm of endometrial cells. After 2, 6, 12, and 24 h of hCG treatment, compared with 1 h, VEGF mRNA was increased by 1.25-fold, 3.19-fold, 4.21-fold, and 4.86-fold and annexin IV by 2.23-fold, 3.37-fold, 5.14-fold, and 5.02-fold. Compared with the control group, annexin IV mRNA and protein were increased in the hCG and hCG/PD98059 groups (mRNA/protein: 1.99-fold/1.80-fold and 2.33-fold/1.93-fold, P < 0.05 ). Compared with the control group, VEGF mRNA and protein were increased in the hCG group (mRNA/protein: 2.30-fold/1.86-fold), but not in the hCG/PD98059 group. Conclusion. hCG could upregulate the mRNA and protein expression of annexin IV and VEGF. The upregulation of annexin IV by hCG could not be inhibited by PD98059, but the upregulation of VEGF by hCG could.

Aggregation of smooth muscle membranes and its use in the preparation of plasma membrane enriched fraction from gastric fundus smooth muscle

1986 ◽  
Vol 64 (6) ◽  
pp. 535-542 ◽  
Author(s):  
Chiu-Yin Kwan
Keyword(s):  
Smooth Muscle ◽  
Plasma Membrane ◽  
Metal Ion ◽  
Gastric Fundus ◽  
Ion Concentration ◽  
Differential Centrifugation ◽  
Induced Membrane ◽  
Membrane Surfaces ◽  
Microsomal Membranes ◽  
Membrane Aggregation

Microsomal membranes isolated from rat gastric fundus smooth muscle by differential centrifugation aggregate substantially in the presence of the divalent metal ion Mg2+ or Ca2+. The magnitude of cation-induced membrane aggregation is higher for Ca2+ than for Mg2+, but the ion concentration required for half-maximum membrane aggregation (K0.5 value) is similar for Mg2+ and Ca2+. Cation-induced membrane aggregation is suppressed by high ionic strength and low pH of the medium. Cation-induced membrane aggregation of mitochondrial membrane and plasma membrane enriched fractions differ in the rate of aggregate formation, metal ion concentration dependence, and pH dependence. Such different properties of membrane aggregation were used to prepare a plasma membrane enriched fraction by conventional differential centrifugation. Subfractionation of the heterogenous microsomal membranes by free-flow electrophoresis indicated that smooth muscle plasma membranes showed a higher electrophoretic mobility than the intracellular membranes. These results suggest that ionic interactions on the cell membrane surfaces differ from those on the intracellular membrane surfaces and that induction of membrane aggregation by Ca2+ or Mg2+ is a useful procedure for an effective and rapid preparation of plasma membrane enriched fraction from smooth muscle.

Differential lipid specificities of the repeated domains of annexin IV

2001 ◽  
Vol 1546 (1) ◽  
pp. 205-215 ◽  
Author(s):  
Hitoshi Sohma ◽  
Carl E Creutz ◽  
Shinsei Gasa ◽  
Hiroko Ohkawa ◽  
Toyoaki Akino ◽  
...  
Keyword(s):  
Annexin Iv

scholarly journals A Potential Endogenous Ligand of Annexin IV in the Exocrine Pancreas

2002 ◽  
Vol 277 (49) ◽  
pp. 47493-47499 ◽  
Author(s):  
Yoko Tsujii-Hayashi ◽  
Mika Kitahara ◽  
Tohru Yamagaki ◽  
Kyoko Kojima-Aikawa ◽  
Isamu Matsumoto
Keyword(s):  
Exocrine Pancreas ◽  
Endogenous Ligand ◽  
Annexin Iv
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