Development of a streak-camera-based time-resolved microscope fluorometer and its application to studies of membrane fusion in single cells

Biochemistry ◽  
1991 ◽  
Vol 30 (26) ◽  
pp. 6517-6527 ◽  
Author(s):  
Akihiro Kusumi ◽  
Akihiko Tsuji ◽  
Masayuki Murata ◽  
Yasushi Sako ◽  
Akiyasu C. Yoshizawa ◽  
...  
Keyword(s):  
Membrane Fusion ◽  
Streak Camera ◽  
Single Cells ◽  
Time Resolved

Time-resolved gain-spectrum measurement scheme using two potential laser media and a streak camera

2001 ◽  
Vol 72 (7) ◽  
pp. 2875-2878
Author(s):  
Toshimasa Kozeki ◽  
Yuji Suzuki ◽  
Zhenlin Liu ◽  
Nobuhiko Sarukura ◽  
Kiyoshi Shimamura ◽  
...  
Keyword(s):  
Streak Camera ◽  
Gain Spectrum ◽  
Time Resolved ◽  
Measurement Scheme ◽  
Spectrum Measurement ◽  
Laser Media

scholarly journals Regulation of transferrin-induced endocytosis by wild-type and C282Y-mutant HFE in transfected HeLa cells

2002 ◽  
Vol 282 (5) ◽  
pp. C973-C979 ◽  
Author(s):  
Lukas Schwake ◽  
Andreas W. Henkel ◽  
Hans D. Riedel ◽  
Thorsten Schlenker ◽  
Matthias Both ◽  
...  
Keyword(s):  
Hela Cells ◽  
Single Cells ◽  
Hereditary Hemochromatosis ◽  
Inhibitory Effect ◽  
Membrane Capacitance ◽  
Wild Type ◽  
Time Resolved ◽  
Cell Membrane Capacitance ◽  
Endocytic Vesicles ◽  
In Cells

The hereditary hemochromatosis protein HFE is known to complex with the transferrin receptor; however, its function regarding endocytosis of transferrin is unclear. We performed patch-clamp capacitance measurements in transfected HeLa cells carrying wild-type or C282Y-mutant HFE cDNA under the control of a tetracycline-sensitive promoter. Whole cell experiments in cells with suppressed expression of wild-type HFE revealed a decrease in membrane capacitance, reflecting predominance of endocytosis in the presence of transferrin. Cells overexpressing C282Y-mutant HFE displayed less intense capacitance decreases, whereas no significant decrease was observed in cells overexpressing wild-type HFE. The formation of single endocytic vesicles in cells with suppressed expression of wild-type HFE was greatly increased in the presence of transferrin as revealed by cell-attached recordings. According to their calculated diameters, many of these vesicles corresponded to clathrin-coated vesicles. These results suggest that wild-type HFE negatively modulates the endocytic uptake of transferrin. This inhibitory effect is attenuated in cells expressing C282Y-mutant HFE. Time-resolved measurements of cell membrane capacitance provide a powerful tool to study transferrin-induced endocytosis in single cells.

scholarly journals Massively parallel and time-resolved RNA sequencing in single cells with scNT-seq

2020 ◽  
Vol 17 (10) ◽  
pp. 991-1001 ◽  
Author(s):  
Qi Qiu ◽  
Peng Hu ◽  
Xiaojie Qiu ◽  
Kiya W. Govek ◽  
Pablo G. Cámara ◽  
...  
Keyword(s):  
Rna Sequencing ◽  
Single Cells ◽  
Massively Parallel ◽  
Time Resolved

Control of Exocytosis in Single Cells

Physiology ◽  
1989 ◽  
Vol 4 (2) ◽  
pp. 53-56 ◽  
Author(s):  
Y Maruyama
Keyword(s):  
Membrane Fusion ◽  
G Proteins ◽  
Secretory Granule ◽  
Single Cells ◽  
Guanine Nucleotide ◽  
Signal Transducers ◽  
Granule Membrane ◽  
Guanine Nucleotide Binding ◽  
Nucleotide Binding Proteins ◽  
Guanine Nucleotide Binding Proteins

Exocytosis can be quantified by measuring changes in membrane capacitance in single internally perfused cells. Exocytosis is controlled by guanine nucleotide-binding proteins (G proteins) acting as key signal transducers. Different G proteins mediate receptor signaling and secretory granule-membrane fusion.

New Data Analysis Method for Time-Resolved Infrared Photoluminescence Spectroscopy

2020 ◽  
pp. 000370282096970
Author(s):  
Kacper Grodecki ◽  
Krzysztof Murawski
Keyword(s):  
Data Analysis ◽  
Photoluminescence Spectrum ◽  
Streak Camera ◽  
Photoluminescence Spectroscopy ◽  
Analysis Method ◽  
Time Resolved ◽  
Time Constants ◽  
Data Treatment ◽  
Typical Time ◽  
Time Resolved Photoluminescence

In this article, a new data treatment based on time-resolved photoluminescence is presented. It works as a streak camera for infrared. A time-resolved photoluminescence spectrum for the HgCd0.33Te0.67 sample at 120 K was performed and analyzed. Typical time-resolved photoluminescence measurements, to compare our results with literature, were conducted. An interpretation of the behavior for three different time constants found in the signal is proposed.

A Streak Camera System for Time Resolved Fluorimetry

1980 ◽  
Vol 13 (11) ◽  
pp. 793-801 ◽  
Author(s):  
G. L. Walden ◽  
J. D. Winefordner
Keyword(s):  
Streak Camera ◽  
Camera System ◽  
Time Resolved
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