Reaction of indole and analogs with amino acid complexes of Escherichia coli tryptophan indole-lyase: detection of a new reaction intermediate by rapid-scanning stopped-flow spectrophotometry

Biochemistry ◽  
1991 ◽  
Vol 30 (24) ◽  
pp. 5927-5934 ◽  
Author(s):  
Robert S. Phillips
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Stopped Flow ◽  
Amino Acid Complexes ◽  
Reaction Intermediate ◽  
Rapid Scanning

scholarly journals An investigation of transient intermediates in the reaction of 2-methylglutamate with glutamate decarboxylase from Escherichia coli

1987 ◽  
Vol 241 (3) ◽  
pp. 699-704 ◽  
Author(s):  
P L Grant ◽  
J M Basford ◽  
R A John
Keyword(s):  
Escherichia Coli ◽  
Glutamate Decarboxylase ◽  
Product Formation ◽  
Stopped Flow ◽  
Spectral Changes ◽  
Early Stages ◽  
Spectral Evidence ◽  
Major Reaction ◽  
Transient Intermediates ◽  
Rapid Scanning

Several intermediates in the reaction of 2-methylglutamate with glutamate decarboxylase from Escherichia coli were detected by stopped-flow spectrophotometry and by rapid-scanning spectrophotometry after conventional mixing. Structures were assigned to intermediates on the basis of kinetic and spectral evidence. In the early stages of the reaction an intermediate with the properties expected of a geminal diamine accumulated significantly. Changes consistent with the conversion of this species into the external aldimine were also observed. The course of product formation was determined and linked with spectral changes taking place in the bound coenzyme. The effect of the minor decarboxylation-dependent transamination that accompanies the major reaction was analysed.

Potentiometric study of enantioselective effects in α-amino acid complexes of copper(II)

1985 ◽  
Vol 104 (1) ◽  
pp. 63-67 ◽  
Author(s):  
I.L. Ulanovski ◽  
A.A. Kurganov ◽  
V.A. Davankov
Keyword(s):  
Amino Acid ◽  
Potentiometric Study ◽  
Amino Acid Complexes

scholarly journals Amino acid substitutions and alteration in cation specificity in the melibiose carrier of Escherichia coli.

1988 ◽  
Vol 263 (28) ◽  
pp. 14276-14280 ◽  
Author(s):  
T Kawakami ◽  
Y Akizawa ◽  
T Ishikawa ◽  
T Shimamoto ◽  
M Tsuda ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Amino Acid Substitutions
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