Structure of the (+)-CC-1065-DNA adduct: critical role of ordered water molecules and implications for involvement of phosphate catalysis in the covalent reaction

Biochemistry ◽  
1991 ◽  
Vol 30 (15) ◽  
pp. 3597-3602 ◽  
Author(s):  
Chin Hsiung Lin ◽  
John M. Beale ◽  
Laurence H. Hurley
Keyword(s):  
Critical Role ◽  
Dna Adduct ◽  
Water Molecules ◽  
Ordered Water

Structures of the complexes of peanut lectin with methyl-β-galactose and N-acetyllactosamine and a comparative study of carbohydrate binding in Gal/GalNAc-specific legume lectins

1999 ◽  
Vol 55 (8) ◽  
pp. 1375-1382 ◽  
Author(s):  
R. Ravishankar ◽  
K. Suguna ◽  
A. Surolia ◽  
M. Vijayan
Keyword(s):  
Comparative Study ◽  
Water Molecules ◽  
Carbohydrate Binding ◽  
Hydroxyl Groups ◽  
Peanut Lectin ◽  
Structural Explanation ◽  
Sugar Ring ◽  
Ordered Water ◽  
The Comparative Study

The crystal structures of complexes of peanut lectin with methyl-β-galactose and N-acetyllactosamine have been determined at 2.8 and 2.7 Å, respectively. These, and the complexes involving lactose and the T-antigenic disaccharide reported previously, permit a detailed characterization of peanut-lectin–carbohydrate association and the role of water molecules therein. The water molecules in the combining site are substantially conserved in the four complexes. The role of interacting sugar hydroxyl groups, when absent, are often mimicked by ordered water molecules not only at the primary combining site, but also at the site of the second sugar ring. The similarity of peanut-lectin–sugar interactions with those in other galactose/N-acetylgalactosamine-specific lectins also extend to a substantial degree to water bridges. The comparative study provides a structural explanation for the exclusive specificity of peanut lectin for galactose at the monosaccharide level, compared with that of the other lectins for galactose as well as N-acetylgalactosamine. The complexes also provide a qualitative structural rationale for differences in the strengths of binding of peanut lectin to different sugars.

scholarly journals Critical Role of Water Molecules in Proton Translocation by the Membrane-Bound Transhydrogenase

Structure ◽  
2017 ◽  
Vol 25 (7) ◽  
pp. 1111-1119.e3 ◽  
Author(s):  
Pius S. Padayatti ◽  
Josephine H. Leung ◽  
Paween Mahinthichaichan ◽  
Emad Tajkhorshid ◽  
Andrii Ishchenko ◽  
...  
Keyword(s):  
Proton Translocation ◽  
Critical Role ◽  
Water Molecules ◽  
Membrane Bound

scholarly journals Active Roles of Water in Aqueous Assembly of Macromolecules

2020 ◽  
Vol 2 (2) ◽  
pp. 17
Keyword(s):  
Self Assembly ◽  
Building Blocks ◽  
Water Molecules ◽  
Range Effect ◽  
Low Field ◽  
Ft Ir ◽  
Low Field Nmr ◽  
Ordered Water ◽  
End Group

Aqueous self-assembly customarily focuses on the molecular interactions of assembling building blocks; the role of water is barely studied. The hydration of hydrophobic P+X- (P+: macromolecular phosphonium cation, X-: anion) is dependent on the ionic end groups, which is responsible for the consequent assembling behavior. The water interaction with the backbone was analyzed by FT-IR, and the dynamics were measured by low field-NMR spectroscopy. The combination of these two techniques reveals the effect of X- on hydration. When X- is I-, the ionic end group ordered water molecules that exerted a detectable long-range effect de-hydrating the backbone. The consequent hydrophobic interaction drove the aqueous assembly of P+I- into micelle-like aggregates with the ionic group exposed to water. In contrast, the ion pair with a hydrophobic anion of [BPh4]- was not able to hold water and did not deplete the hydration water. The hydrated backbone of P+[BPh4]- assembled into vesicles that were driven by hydration interactions. This elucidation at the molecular level is craved to progress aqueous supramolecular chemistry.

The role of water molecules in phototransduction of retinal proteins and G protein-coupled receptors

2018 ◽  
Vol 207 ◽  
pp. 27-37 ◽  
Author(s):  
Elena Lesca ◽  
Valérie Panneels ◽  
Gebhard F. X. Schertler
Keyword(s):  
G Protein ◽  
Critical Role ◽  
G Protein Coupled Receptors ◽  
Water Molecules ◽  
Important Class ◽  
Retinal Proteins ◽  
G Protein Coupled

Water molecules play a critical role during activation of GPCRs, one of the most important class of signalling proteins.

The Role of the SLP in Autism Spectrum Disorder Screening and Assessment

2008 ◽  
Vol 15 (2) ◽  
pp. 50-59 ◽  
Author(s):  
Amy Philofsky
Keyword(s):  
Language Development ◽  
Critical Role ◽  
Children With Autism ◽  
Autism Spectrum ◽  
Children With Asd ◽  
Prevalence Estimates ◽  
Notable Increase ◽  
Screening Assessment ◽  
Critical Components

AbstractRecent prevalence estimates for autism have been alarming as a function of the notable increase. Speech-language pathologists play a critical role in screening, assessment and intervention for children with autism. This article reviews signs that may be indicative of autism at different stages of language development, and discusses the importance of several psychometric properties—sensitivity and specificity—in utilizing screening measures for children with autism. Critical components of assessment for children with autism are reviewed. This article concludes with examples of intervention targets for children with ASD at various levels of language development.

A critical role of iNOS-derived Nitric Oxide (NO) and progesterone in implantation

1998 ◽  
Vol 5 (1) ◽  
pp. 115A-115A
Author(s):  
K CHWALISZ ◽  
E WINTERHAGER ◽  
T THIENEL ◽  
R GARFIELD
Keyword(s):  
Nitric Oxide ◽  
Critical Role
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