Temperature dependence of the rates of conformational changes reported by fluorescein 5'-isothiocyanate modification of the hydrogen ion-potassium- and sodium-potassium-ATPases

Biochemistry ◽  
1991 ◽  
Vol 30 (14) ◽  
pp. 3503-3510 ◽  
Author(s):  
Larry D. Faller ◽  
Ruben A. Diaz ◽  
Georgios Scheiner-Bobis ◽  
Robert A. Farley

The distributions in angle and energy for charged particles emitted from polycrystalline platinum bombarded with ions of hydrogen, sodium, potassium and some hydrocarbons, have been measured, in continuation of previous work (Cawthron, Cotterell & Oliphant 1969 a , b , parts I and II; 1970, part III) to lower energies. Composite spectra are presented, including ions of both signs. The flux of emitted charged particles, under hydrogen ion bombardment, is shown to contain approximately equal numbers of protons and H¯ ions, except at the lowest bombarding energies, where the latter apparently predominate. Scattering in all cases is shown to increase with bombarding energy over the range covered, the increase being near linear for hydrogen and very rapid for alkalimetal ions. In all cases the total emission of charged particles is very small at the lowest bombarding energies employed.


1967 ◽  
Vol 33 (2) ◽  
pp. 395-410 ◽  
Author(s):  
David W. Deamer ◽  
Antony Crofts

Addition of Triton X-100 to chloroplast suspensions to a final concentration of 100–200 µM causes an approximate tripling of chloroplast volume and complete inhibition of light-induced conformational changes, light-dependent hydrogen ion transport, and photophosphorylation. Electron microscopic studies show that chloroplasts treated in this manner manifest extensive swelling in the form of vesicles within their inner membrane structure. Triton was adsorbed to chloroplast membranes in a manner suggesting a partition between the membrane phase and the suspending medium, rather than a strong, irreversible binding. This adsorption results in the production of pores through which ions may freely pass, and it is suggested that the inhibition of conformational changes, hydrogen ion transport, and photophosphorylation by Triton is due to an inability of treated chloroplast membranes to maintain a light-dependent pH gradient. The observed swelling is due to water influx in response to a fixed, osmotically active species within the chloroplasts, after ionic equilibrium has occurred. This is supported by the fact that chloroplasts will shrink upon Triton addition if a nonpenetrating, osmotically active material such as dextran or polyvinylpyrrolidone is present externally in sufficient concentration (>0.1 mM) to offset the osmotic activity of the internal species.


2014 ◽  
Vol 314 (9) ◽  
pp. 1300-1318 ◽  
Author(s):  
A.-K. Schaffer ◽  
E. Petrishcheva ◽  
G. Habler ◽  
R. Abart ◽  
D. Rhede ◽  
...  

2018 ◽  
Vol 60 (11) ◽  
pp. 2116
Author(s):  
Е.Е. Пестов ◽  
Ю.Н. Ноздрин ◽  
А.И. Елькина ◽  
Ю.C. Ерин ◽  
М. Лю ◽  
...  

Abstract —Temperature dependences of the third-harmonic power for the (Na_0.3K_0.7)_ x Fe_2 – _ y Se_2 sodium–potassium ferroselenide single crystals have been investigated by nonlinear near-field microwave microscopy. The temperature dependence of magnetic susceptibility at a frequency of 100 kHz for this compound has been measured. The experimental results suggest a double-gap structure of the (Na_0.3K_0.7)_ x Fe_2 – _ y Se_2 superconductor.


2010 ◽  
Vol 114 (15) ◽  
pp. 5066-5081 ◽  
Author(s):  
Biswajit Guchhait ◽  
Harun Al Rasid Gazi ◽  
Hemant K. Kashyap ◽  
Ranjit Biswas

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