Stimulation of the ATPase activity of rat brain protein kinase C by phospho acceptor substrates of the enzyme

Biochemistry ◽  
1991 ◽  
Vol 30 (9) ◽  
pp. 2549-2554 ◽  
Author(s):  
Catherine A. O'Brian ◽  
Nancy E. Ward
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Atpase Activity ◽  
Rat Brain ◽  
Brain Protein ◽  
Kinase C ◽  
Stimulation Of

Rat Brain Protein Kinase C: Purification, Antibody Production, and Quantification in Discrete Regions of Hippocampus

1989 ◽  
Vol 52 (1) ◽  
pp. 215-221 ◽  
Author(s):  
Bryan L. Roth ◽  
John P. Mehegan ◽  
David M. Jacobowitz ◽  
Frank Robey ◽  
Michael J. Iadarola
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Rat Brain ◽  
Antibody Production ◽  
Brain Protein ◽  
Kinase C

Endothelin stimulates Na(+)-K(+)-ATPase activity by a protein kinase C-dependent pathway in rabbit aorta

1991 ◽  
Vol 261 (1) ◽  
pp. H38-H45 ◽  
Author(s):  
S. Gupta ◽  
N. B. Ruderman ◽  
E. J. Cragoe ◽  
I. Sussman
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Atpase Activity ◽  
Rabbit Aorta ◽  
Effective Concentration ◽  
Kinase C ◽  
Exchange Inhibitor ◽  
Half Maximal Effective Concentration ◽  
Dependent Pathway ◽  
Stimulation Of

Incubation with endothelin (Endo) caused a time- and concentration-dependent increase in both ouabain-sensitive (OS) and ouabain-insensitive (OI) 86Rb+ uptake [half-maximal effective concentration (EC50) for OS component = 11 nM] in the rabbit aorta. Increase in the OS component [Na(+)-K(+)-adenosine triphosphatase (ATPase) activity] accounted for 70% of the 110% increase in total 86Rb+ uptake at a maximally effective concentration of Endo (100 nM). Protein kinase C (PKC) activator phorbol 12,13-dibutyrate (PDBU; 100 nM) increased total 86Rb+ uptake by 69%, with 42% of the increase in the OS component. Stimulation by Endo and PDBU was not additive. Staurosporine (STA; 100 nM) inhibited stimulation of total 86Rb+ uptake by Endo and PDBU by approximately 60%. With ouabain and STA added together, inhibition of Endo-stimulated total 86Rb+ uptake (90%) was greater than with either agent alone, suggesting that STA inhibits an OS as well as an OI component of 86Rb+ uptake. Stimulation of total 86Rb+ uptake by both Endo and PDBU were also inhibited by approximately 60% by the Na(+)-H+ exchange inhibitor 5-(N-ethyl-N-isopropyl)amiloride (EIPA). Endo-stimulated total 86Rb+ uptake was not further inhibited when ouabain was added together with EIPA, suggesting that Na(+)-H+ exchange is primarily linked to the OS component of 86Rb+ uptake. In contrast, Na(+)-K(+)-Cl- cotransport inhibitor bumetanide inhibited increases in total 86Rb+ uptake caused by Endo (30%) and PDBU (56%) due solely to its effects on OI 86Rb+ uptake. Results suggest that Endo stimulates Na(+)-K(+)-ATPase activity in rabbit aorta by activating PKC and Na(+)-H+ exchange.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Aldosterone stimulates vacuolar H+-ATPase activity in renal acid-secretory intercalated cells mainly via a protein kinase C-dependent pathway

2011 ◽  
Vol 301 (5) ◽  
pp. C1251-C1261 ◽  
Author(s):  
Christian Winter ◽  
Nicole B. Kampik ◽  
Luca Vedovelli ◽  
Florina Rothenberger ◽  
Teodor G. Păunescu ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Atpase Activity ◽  
Intracellular Signaling ◽  
Collecting Duct ◽  
Intercalated Cells ◽  
Nongenomic Action ◽  
Kinase C ◽  
Urinary Acidification ◽  
Stimulation Of

Urinary acidification in the collecting duct is mediated by the activity of H+-ATPases and is stimulated by various factors including angiotensin II and aldosterone. Classically, aldosterone effects are mediated via the mineralocorticoid receptor. Recently, we demonstrated a nongenomic stimulatory effect of aldosterone on H+-ATPase activity in acid-secretory intercalated cells of isolated mouse outer medullary collecting ducts (OMCD). Here we investigated the intracellular signaling cascade mediating this stimulatory effect. Aldosterone stimulated H+-ATPase activity in isolated mouse and human OMCDs. This effect was blocked by suramin, a general G protein inhibitor, and GP-2A, a specific Gαq inhibitor, whereas pertussis toxin was without effect. Inhibition of phospholipase C with U-73122, chelation of intracellular Ca2+ with BAPTA, and blockade of protein kinase C prevented the stimulation of H+-ATPases. Stimulation of PKC by DOG mimicked the effect of aldosterone on H+-ATPase activity. Similarly, aldosterone and DOG induced a rapid translocation of H+-ATPases to the luminal side of OMCD cells in vivo. In addition, PD098059, an inhibitor of ERK1/2 activation, blocked the aldosterone and DOG effects. Inhibition of PKA with H89 or KT2750 prevented and incubation with 8-bromoadenosine-cAMP mildly increased H+-ATPase activity. Thus, the nongenomic modulation of H+-ATPase activity in OMCD-intercalated cells by aldosterone involves several intracellular pathways and may be mediated by a Gαq protein-coupled receptor and PKC. PKA and cAMP appear to have a modulatory effect. The rapid nongenomic action of aldosterone may participate in the regulation of H+-ATPase activity and contribute to final urinary acidification.

Increased phosphorylation of nuclear substrates for rat brain protein kinase C in regenerating rat liver nuclei

1992 ◽  
Vol 4 (3) ◽  
pp. 313-319 ◽  
Author(s):  
Meri Mazzoni ◽  
Cinzia Carini ◽  
Alessandro Matteucci ◽  
Alberto Maria Martelli ◽  
Valeria Bertagnolo ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Rat Brain ◽  
Rat Liver ◽  
Brain Protein ◽  
Rat Liver Nuclei ◽  
Kinase C ◽  
Regenerating Rat Liver ◽  
Liver Nuclei

scholarly journals Two types of complementary DNAs of rat brain protein kinase C

FEBS Letters ◽  
1986 ◽  
Vol 206 (2) ◽  
pp. 347-352 ◽  
Author(s):  
Yoshitaka Ono ◽  
Tsutomu Kurokawa ◽  
Tomoko Fujii ◽  
Kenji Kawahara ◽  
Koichi Igarashi ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Rat Brain ◽  
Brain Protein ◽  
Kinase C

In vitro inhibition of rat brain protein kinase C by rhodamine 6G

1987 ◽  
Vol 36 (8) ◽  
pp. 1231-1235 ◽  
Author(s):  
Catherine A. O'Brian ◽  
I.Bernard Weinstein
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Rat Brain ◽  
Rhodamine 6G ◽  
Brain Protein ◽  
Kinase C ◽  
In Vitro Inhibition
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