Role of phosphatidylethanol in membranes. Effects on membrane fluidity, tolerance to ethanol, and activity of membrane-bound enzymes

Biochemistry ◽  
1991 ◽  
Vol 30 (9) ◽  
pp. 2477-2482 ◽  
Author(s):  
Fausta Omodeo-Sale ◽  
Clara Lindi ◽  
Paola Palestini ◽  
Massimo Masserini
Keyword(s):  
Membrane Fluidity ◽  
Membrane Bound ◽  
Membrane Bound Enzymes ◽  
Tolerance To Ethanol

Alterations in rat erythrocyte membrane due to hexachlorocyclohexane (technical) exposure

1998 ◽  
Vol 17 (11) ◽  
pp. 638-642 ◽  
Author(s):  
Pankaj Bhalla ◽  
Deepa Agrawal
Keyword(s):  
Membrane Fluidity ◽  
Erythrocyte Membrane ◽  
Osmotic Fragility ◽  
Structure And Function ◽  
Membrane Structure And Function ◽  
Hydrophobic Molecule ◽  
Erythrocyte Membrane Fluidity ◽  
Membrane Bound ◽  
And Function ◽  
Membrane Bound Enzymes

1 Hexachlorocyclohexane (HCH), an organochlorine pesticide having hydrophobic molecule is known to act on membranes. HCH mediated alterations in erythrocyte membrane occur through disorganization of the lipid bilayer. Therefore the changes in erythrocyte membrane fluidity, osmotic fragility and certain membrane bound enzymes were studied. Administration of HCH (technical) to rats at 5 mg/kg, orally, 5 days a week for 1, 2 and 3 months caused marked increase in erythrocyte membrane fluidity, osmotic fragility anddecreaseinlevelsofNa+, K+-ATPase, acetylcholinesterase in erythrocytes and glutathione in blood. 2 These changes indicate that HCH adversely affects membrane structure and function.

scholarly journals (2-Aminobenzothiazole)-Methyl-1,1-Bisphosphonic Acids: Targeting Matrix Metalloproteinase 13 Inhibition to the Bone

2021 ◽  
Vol 14 (2) ◽  
pp. 85
Author(s):  
Antonio Laghezza ◽  
Luca Piemontese ◽  
Leonardo Brunetti ◽  
Alessia Caradonna ◽  
Mariangela Agamennone ◽  
...  
Keyword(s):  
Acid Group ◽  
Biological Evaluation ◽  
Matrix Metalloproteinase 13 ◽  
In Silico Studies ◽  
Long Time ◽  
Membrane Bound ◽  
Bisphosphonic Acids ◽  
Membrane Bound Enzymes

Matrix Metalloproteinases (MMPs) are a family of secreted and membrane-bound enzymes, of which 24 isoforms are known in humans. These enzymes degrade the proteins of the extracellular matrix and play a role of utmost importance in the physiological remodeling of all tissues. However, certain MMPs, such as MMP-2, -9, and -13, can be overexpressed in pathological states, including cancer and metastasis. Consequently, the development of MMP inhibitors (MMPIs) has been explored for a long time as a strategy to prevent and hinder metastatic growth, but the important side effects linked to promiscuous inhibition of MMPs prevented the clinical use of MMPIs. Therefore, several strategies were proposed to improve the therapeutic profile of this pharmaceutical class, including improved selectivity toward specific MMP isoforms and targeting of specific organs and tissues. Combining both approaches, we conducted the synthesis and preliminary biological evaluation of a series of (2-aminobenzothiazole)-methyl-1,1-bisphosphonic acids active as selective inhibitors of MMP-13 via in vitro and in silico studies, which could prove useful for the treatment of bone metastases thanks to the bone-targeting capabilities granted by the bisphosphonic acid group.

scholarly journals Alterations in the activities of hepatic plasma-membrane and microsomal enzymes during liver regeneration

1983 ◽  
Vol 212 (2) ◽  
pp. 445-452 ◽  
Author(s):  
G Deliconstantinos ◽  
G Ramantanis
Keyword(s):  
Plasma Membrane ◽  
Liver Regeneration ◽  
Membrane Fluidity ◽  
Plasma Membranes ◽  
Membrane Lipids ◽  
Biological Significance ◽  
Hill Coefficient ◽  
Membrane Bound ◽  
The Hill ◽  
Membrane Bound Enzymes

A marked increase in the activities of rat liver plasma-membrane (Na+ + K+)-stimulated ATPase and microsomal Ca2+-stimulated ATPase was observed 18h after partial hepatectomy. Lipid analyses for both membrane preparations reveal that in partially hepatectomized rats the cholesterol and sphingomyelin content are decreased with a subsequent decrease in the cholesterol/phospholipid molar ratio compared with those of sham-operated animals. Changes in the allosteric properties of plasma-membrane (Na+ + K+)-stimulated ATPase by F- (as reflected by changes in the Hill coefficient) indicated a fluidization of the lipid bilayer of both membrane preparations in 18 h-regenerating liver. The amphipathic dodecyl glucoside incorporated into the hepatic plasma membranes evoked a marked increase in the (Na+ + K+)-stimulated ATPase and 5′-nucleotidase activities. The lack of effect of the glucoside on the Lubrol-PX-solubilized 5′-nucleotidase indicates that changes in the activities of the membrane-bound enzymes caused by the glucoside are due to modulation of the membrane fluidity. Dodecyl glucoside appears to increase the membrane fluidity, evaluated through changes in the Hill coefficient for plasma-membrane (Na+ + K+)-stimulated ATPase. The biological significance of these data is discussed in terms of the differences and changes in the interaction of membrane-bound enzymes with membrane lipids during liver regeneration.

Role of lindane in membranes. Effects on membrane fluidity and activity of membrane-bound proteins

1994 ◽  
Vol 14 (3) ◽  
pp. 131-138 ◽  
Author(s):  
P. López-Aparicio ◽  
M. N. Recio ◽  
J. C. Prieto ◽  
M. A. Pérez-Albarsanz
Keyword(s):  
Membrane Fluidity ◽  
Adrenergic Receptor ◽  
Plasma Membranes ◽  
Opposite Effect ◽  
Glucose Transporter ◽  
Regulatory Mechanism ◽  
Cyclic Amp Accumulation ◽  
Membrane Bound ◽  
The Difference

The influence of lindane (gamma-hexachlorocyclohexane) on fluidity of plasma membranes from rat renal cortical tubules has been investigated. Preincubation with lindane increased membrane fluidity. This effect was accompanied by (i) a decrease in the transport of glucose with regard to the controls and (ii) an inhibition of the β-adrenergic stimulatory activity upon cyclic AMP accumulation. However, a significant decrease of the membrane fluidity was found when rats were injected with lindane for 12 days. The injection of lindane exerted the opposite effect on the membrane proteins, the glucose transporter and the β-adrenergic receptor, enhancing the glucose uptake and increasing the isoproterenol-stimulated cycle AMP accumulation. A possible explanation of the difference could involve a resistance to membrane disordering by lindane through a regulatory mechanism that would balance the activity of many lindane-sensitive proteins in insecticide-injected rats.

Sign in / Sign up

Export Citation Format

Share Document