Purification and characterization of dihydroorotate dehydrogenase from the rodent malaria parasite Plasmodium berghei

Biochemistry ◽  
1991 ◽  
Vol 30 (7) ◽  
pp. 1934-1939 ◽  
Author(s):  
Jerapan Krungkrai ◽  
Anthony Cerami ◽  
Graeme B. Henderson
Keyword(s):  
Plasmodium Berghei ◽  
Malaria Parasite ◽  
Dihydroorotate Dehydrogenase ◽  
Rodent Malaria Parasite ◽  
Purification And Characterization ◽  
Rodent Malaria ◽  
Parasite Plasmodium

scholarly journals Enzymatic Characterization of Recombinant Food Vacuole Plasmepsin 4 from the Rodent Malaria Parasite Plasmodium berghei

PLoS ONE ◽  
2015 ◽  
Vol 10 (10) ◽  
pp. e0141758 ◽  
Author(s):  
Peng Liu ◽  
Arthur H. Robbins ◽  
Melissa R. Marzahn ◽  
Scott H. McClung ◽  
Charles A. Yowell ◽  
...  
Keyword(s):  
Plasmodium Berghei ◽  
Malaria Parasite ◽  
Food Vacuole ◽  
Enzymatic Characterization ◽  
Rodent Malaria Parasite ◽  
Rodent Malaria ◽  
Parasite Plasmodium

Expression profiles of peroxiredoxins in liver stage of the rodent malaria parasite Plasmodium berghei

2013 ◽  
Vol 62 (3) ◽  
pp. 337-340 ◽  
Author(s):  
Miho Usui ◽  
Hirono Masuda-Suganuma ◽  
Shinya Fukumoto ◽  
Jose Ma. M. Angeles ◽  
Noboru Inoue ◽  
...  
Keyword(s):  
Plasmodium Berghei ◽  
Malaria Parasite ◽  
Expression Profiles ◽  
Rodent Malaria Parasite ◽  
Liver Stage ◽  
Rodent Malaria ◽  
Parasite Plasmodium

Effect of thioredoxin peroxidase-1 gene disruption on the liver stages of the rodent malaria parasite Plasmodium berghei

2015 ◽  
Vol 64 (3) ◽  
pp. 290-294 ◽  
Author(s):  
Miho Usui ◽  
Hirono Masuda-Suganuma ◽  
Shinya Fukumoto ◽  
Jose Ma. M. Angeles ◽  
Hassan Hakimi ◽  
...  
Keyword(s):  
Plasmodium Berghei ◽  
Gene Disruption ◽  
Malaria Parasite ◽  
Rodent Malaria Parasite ◽  
Rodent Malaria ◽  
Parasite Plasmodium ◽  
Thioredoxin Peroxidase

scholarly journals Depressed malarial immunity in pregnant mice

1980 ◽  
Vol 28 (2) ◽  
pp. 630-632
Author(s):  
A A van Zon ◽  
W M Eling
Keyword(s):  
Plasmodium Berghei ◽  
Malaria Parasite ◽  
Rodent Malaria Parasite ◽  
Rodent Malaria ◽  
Parasite Plasmodium ◽  
Pregnant Mice

A proportion of mice solidly immune to the rodent malaria parasite Plasmodium berghei exhibited a pregnancy-associated depressed immunity with a transient or even lethal recrudescence.

Induction of Humoral Immune Response by 66 kDa Protein and Amplification of Gene present by using Polymerase Chain Reaction

2017 ◽  
Vol 6 (6) ◽  
pp. 206-211
Author(s):  
Nisha Devi ◽  
H. S. Banal
Keyword(s):  
Polymerase Chain Reaction ◽  
Plasmodium Berghei ◽  
Genomic Dna ◽  
Malaria Parasite ◽  
Rodent Malaria Parasite ◽  
Chain Reaction ◽  
Rodent Malaria ◽  
Humoral Immune ◽  
Parasite Plasmodium ◽  
Polymerase Chain

Malaria is one of the most dreaded vector borne disease caused by apicomplexan parasite, of the genus Plasmodium. In the present study the gene which encodes 66kDa protein in rodent malaria parasite, Plasmodium berghei, was cloned. Rodent malaria parasite, Plasmodium berghei (NK-65), was found lethal to white Swiss mice (BALB/c). The immunogenicity and protective efficacy of purified 66 kDa protein was evaluated both in vitro and in vivo. Mice immunized with 66 kDa protein induced a strong humoral immune response, revealing role of 66 kDa in malaria control. Genomic DNA of P. berghei was isolated from cell free parasite. Genomic DNA serves as a template for isola on of gene encoding 66 kDa protein. Further, the gene was amplified by using polymerase chain reaction. The amplified DNA fragment of 1717 bp was extracted from agarose gel and puri ed. The purified gene was ligated to a cloning vector for cloning procedure by TA-cloning mechanism. Results of this study provide evidence that 66 kDa protein is highly immunogenic during experimental infec on in rodents and such an gen will be a suitable candidate malaria vaccine. 

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