Kinetic evidence for two nucleotide binding sites on the calcium ATPase of sarcoplasmic reticulum

Biochemistry ◽  
1991 ◽  
Vol 30 (6) ◽  
pp. 1456-1461 ◽  
Author(s):  
Richard J. Coll ◽  
Alexander J. Murphy
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Binding Sites ◽  
Nucleotide Binding ◽  
Calcium Atpase ◽  
Nucleotide Binding Sites

scholarly journals Probing the Nucleotide Binding Sites of Sarcoplasmic Reticulum Atpase by Photoaffinity Labeling

1986 ◽  
Vol 49 (1) ◽  
pp. 108-109 ◽  
Author(s):  
S. Verjovski-Almeida ◽  
P.C. Carvalho-Alves ◽  
C.G. Oliveira ◽  
S.T. Ferreira
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Binding Sites ◽  
Photoaffinity Labeling ◽  
Nucleotide Binding ◽  
Nucleotide Binding Sites

scholarly journals Dependence on Membrane Lipids of the Effect of Vanadate on Calcium and ATP Binding to Sarcoplasmic Reticulum ATPase

1984 ◽  
Vol 39 (11-12) ◽  
pp. 1137-1140 ◽  
Author(s):  
Pankaj Medda ◽  
Wilhelm Hasselbach
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Binding Sites ◽  
Calcium Binding ◽  
Membrane Lipids ◽  
Nucleotide Binding ◽  
Atp Binding ◽  
Transport Atpase ◽  
Nucleotide Binding Sites ◽  
High Affinity ◽  
Calcium Binding Sites

Abstract The affinity of the sarcoplasmic reticulum transport ATPase for calcium and ATP is not affected by lipid depriviation while vanadate binding is completely abolished. Lipid substitution restores vanadate binding as well as the vanadate induced disappearance of the enzyme’s high affinity calcium and nucleotide binding sites. Nucleotide binding is simultaneously restored with the displacement of vanadate from the enzyme following the occupation of its low affinity calcium binding sites.

scholarly journals Synthesis of spin-labeled 2-azido-ATP: Evidence for distinct nucleotide-binding sites in calcium pump protein from sarcoplasmic reticulum

FEBS Letters ◽  
1989 ◽  
Vol 254 (1-2) ◽  
pp. 8-12 ◽  
Author(s):  
Pia Jakobs ◽  
Hubertus E. Sauer ◽  
J.Oliver McIntyre ◽  
Sidney Fleischer ◽  
Wolfgang E. Trommer
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Binding Sites ◽  
Nucleotide Binding ◽  
Calcium Pump ◽  
Nucleotide Binding Sites
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