N-Terminal Intramolecularly Conserved Histidines of Three Domains inGonylaulaxLuciferase Are Responsible for Loss of Activity in the Alkaline Region†

Biochemistry ◽  
2001 ◽  
Vol 40 (6) ◽  
pp. 1844-1849 ◽  
Author(s):  
Liming Li ◽  
Liyun Liu ◽  
Robert Hong ◽  
Deborah Robertson ◽  
J. Woodland Hastings
Keyword(s):  
Alkaline Region

Effects of neonatal androgenization on the chromatofocusing pattern of anterior pituitary FSH in the female rat

1990 ◽  
Vol 126 (2) ◽  
pp. 323-332 ◽  
Author(s):  
A. Ulloa-Aguirre ◽  
P. Damián-Matsumura ◽  
R. Espinoza ◽  
R. Dominguez ◽  
L. Morales ◽  
...  
Keyword(s):  
Anterior Pituitary ◽  
Female Rats ◽  
Size Exclusion ◽  
Male Rats ◽  
Female Rat ◽  
Adult Males ◽  
Multiple Forms ◽  
Alkaline Region ◽  
Pituitary Glands ◽  
Ph Range

ABSTRACT Anterior pituitary glands were removed from neonatally androgenized (100 μg testosterone propionate) female rats and normal controls at 5, 10, 18, 21, 30, 60 and 90 days of age, and the multiple forms of FSH present within them were separated by chromatofocusing (pH range 7·5–4·0). Additional pituitary glands from intact adult males (90 days old) were also studied for comparative purposes. All animal groups exhibited multiple forms of immunoactive FSH within a pH range of 7·5–4·0, as well as an additional FSH form obtained after the addition of 1·0 mol NaCl/l to the chromatofocusing column (salt peak). In animals 5–30 days old (controls and androgenized) the majority of FSH applied to the chromatofocusing columns was recovered within the salt peak (45-85% of total FSH immunoactivity recovered). However, as the animals aged, more FSH immunoactivity focused within less acidic regions (isoelectric point (pI) 5·9–5·0); pituitaries from animals 60 days old contained the greatest proportion of FSH focused within this pH range (controls, 39·2±0·6%; androgenized, 23·1 ±0·9% of total immunoactivity recovered; P < 0·03 vs animals 30 days old for both experimental groups). This shift towards less acidic FSH was attenuated in androgenized animals compared with the controls (P<0·01). In control adult rats, the chromatofocusing distribution pattern of pituitary FSH varied according to the day of the oestrous cycle. Pituitary extracts from control rats decapitated during the morning of pro-oestrus, oestrus and day 1 of dioestrus exhibited the highest proportion of immunoactive FSH (23·2–28·8% of total) focused within a pH range of 5·9–5·0, whilst only 10·4–11·6% of FSH from androgenized rats and those on day 1 of dioestrus was recovered within this pH range (P<0·05). In control animals decapitated during the morning of prooestrus and oestrus, 10–26% of FSH focused within the most alkaline region (pI 7·5–6·0); the chromatofocusing pattern of pituitary FSH from the neonatally androgenized animals was characteristic, in that no more than one peak (1·5±0·5% of total) was detected in this alkaline region. In the adult male rats, the majority of pituitary FSH eluted from the chromatofocusing columns within a pH of 4·9–4·0 (52·4±1·2% of total FSH immunoactivity) and the salt peak (pH <4·0) (33·1 ±2·4 of total). All FSH isoforms obtained after chromatofocusing represented α and β dimers as disclosed by size exclusion chromatography. The results strongly suggest that a cyclic or 'female' pattern of hypothalamic and gonadal secretion leads the anterior pituitary towards the production of less acidic FSH isoforms, whereas a tonic or 'androgenic' type of secretion, as that present in adult males and females with the androgen-induced anovulatory syndrome, leads more to the production of strongly acidic FSH isoforms. The finding of qualitative and quantitative differences among normally cycling and androgenized animals gives further support for the concept of the existence of a sexual dichotomy in terms of the type of FSH synthesized by the anterior pituitary gland. Journal of Endocrinology (1990) 126, 323–332

Structural transition of bovine plasma albumin in the alkaline region the N-B transition

2009 ◽  
Vol 35 (1) ◽  
pp. 1-11 ◽  
Author(s):  
SEIICHI ERA ◽  
KIYOOMI B. ITOH ◽  
MASARU SOGAMI ◽  
KAZUO KUWATA ◽  
TORU IWAMA ◽  
...  
Keyword(s):  
Structural Transition ◽  
Plasma Albumin ◽  
Alkaline Region ◽  
Bovine Plasma

Electrolytic coatings (layers) deposited from palladium(II) salts

1991 ◽  
Vol 56 (1) ◽  
pp. 167-177
Author(s):  
Jaromír Hlavatý ◽  
Jiří Volke
Keyword(s):  
Active Layer ◽  
Glassy Carbon ◽  
Oxidative Activity ◽  
Voltammetric Curve ◽  
Alkaline Region ◽  
Linear Behaviour ◽  
Electrolytic Preparation ◽  
Current Densities ◽  
Palladium Oxides ◽  
Ph Range

In contrast to a number of investigations concerning preparation of layers and coatings of palladium(II) oxides this communication describes a reproducible electrolytic preparation from activation baths containing Pd(II) ions of coatings with hydrated palladium oxides, in particular on platinum supports. The voltammetric curves of coatings formed in such a way are reproducible over the whole pH range and exhibit an irreversible cathodic-anodic couple of peaks. For the voltammetric curve on an active layer deposited on a platinum support from a solution of Pd(NO3)2 at pH 7.03 Ekp is + 0.02 V and Eap +0.33 V (vs SCE). The observed character of the pH dependences for the peak potentials and current densities with a similar, mostly linear, behaviour has been also observed with layers deposited on glassy carbon or nickel. The deposited layers containing hydrated palladium oxides exhibit oxidative activity as follows from voltammograms in slightly acidic to neutral and strongly alkaline region. These curves point to the ability to oxidize not only ethanol but also toluene used as a with difficulty oxidizable model substrate.

Study on pH Fluorescent Probe Based on Exocyclic Ring-Fused Boron Dipyrromethene Fluorophore

2012 ◽  
Vol 487 ◽  
pp. 81-88
Author(s):  
Hai Yun Xu ◽  
Qi Feng Liu ◽  
Wen Xian Zhao ◽  
Cui Lan Feng
Keyword(s):  
High Temperature ◽  
Fluorescent Probe ◽  
Design Principle ◽  
Aromatic Aldehydes ◽  
Fluorescence Properties ◽  
Ph Sensing ◽  
Synthetic Route ◽  
Alkaline Region ◽  
Sensing Mechanism ◽  
Boron Dipyrromethene

Compound d was prepared by the improved three-step synthetic route using cyclohexene as starting material. After treatment of d with excess LiAlH4 at high temperature, the obtained e was reacted with various kinds of aromatic aldehydes including 4-N, N-dimethylaminobenzaldehyde, 4-hydroxybenzaldehyde, 4-methoxybenzaldehyde, benzaldehyde and 4-formyl-benzo-15-crown-5, respectively, in the presence of CF3COOH and oxidated with DDQ, followed by treatment with NEt3 and BF3.Et2O to provide five kinds of BODIPY derivatives fused with exocyclic rings. Their absorption and fluorescence properties were investigated. The fluorometric titrations were performed to examine their responsive abilities to pH in CH3OH-H2O (1:1, V/V). The results indicate that BODIPY 1 and 2 can be applied as a fluorescent probe for pH sensing in the acid and alkaline region, respectively, and switching between the fluorescence “ON/OFF” states in BODIPY 1 or 2 can be repeated by modulation of protonation/deprotonation process without decomposition and formation of precipitates. Moreover, the design principle and sensing mechanism of two probes are also preliminarily discussed.

scholarly journals A high abundance of Firmicutes in the intestine of chinese mitten crabs (Eriocheir sinensis) cultured in an alkaline region

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Weibing Guan ◽  
Kui Li ◽  
Shuang Zhao ◽  
Kejun Li
Keyword(s):  
Alpha Diversity ◽  
Illumina Miseq ◽  
Northwest China ◽  
Eriocheir Sinensis ◽  
Pond Aquaculture ◽  
Alkaline Region ◽  
Intestinal Bacterial Community ◽  
Mitten Crab ◽  
Mitten Crabs ◽  
Length Width

AbstractThe Chinese mitten crab (Eriocheir sinensis) is a popular aquaculture product in East Asia, especially in China. In the last decade, rice–crab co-culture has rapidly expanded in China. Under this model, crabs are raised in rice fields instead of in traditional aquaculture ponds. In this study, we cultured two varieties of Chinese mitten crabs (Changjiang and Liaohe) in an alkaline region in northwest China and used Illumina MiSeq sequencing to compare the intestinal bacterial alpha diversity and community structure between traditional and co-culture aquaculture models, between two crab varieties, and between female and male crabs. Significant variations in intestinal bacterial communities were found between crab varieties and between female and male crabs but not between aquaculture models. These results show that rice–crab co-culture operations did not obviously impact the crab intestinal bacterial community compared with traditional pond aquaculture. Firmicutes was the most abundant bacterial phylum in the crab intestines (78%, relative abundance). Three dominant operational taxonomic units (OTUs) represented 73.2% of Firmicutes sequences and 56.8% of all sequences. A dominant OTU assigned as Firmicutes that was negatively correlated with crab body length, width, and weight was found in the source water for the experimental area. The results of this study suggest that the aquaculture of Chinese mitten crabs in alkaline regions requires more study to improve cultivation techniques.

scholarly journals Colour and stability of pure anthocyanins influenced by pH including the alkaline region

1998 ◽  
Vol 63 (4) ◽  
pp. 435-440 ◽  
Author(s):  
Torgils Fossen ◽  
Luis Cabrita ◽  
Oyvind M. Andersen
Keyword(s):  
Alkaline Region
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