Characterization of Saxitoxin Binding to Saxiphilin, a Relative of the Transferrin Family That Displays pH-Dependent Ligand Binding

Biochemistry ◽  
1994 ◽  
Vol 33 (40) ◽  
pp. 12312-12322 ◽  
Author(s):  
Lyndon E. Llewellyn ◽  
Edward G. Moczydlowski
Keyword(s):  
Ligand Binding ◽  
Ph Dependent

scholarly journals Odorant-binding protein. Characterization of ligand binding.

1990 ◽  
Vol 265 (11) ◽  
pp. 6118-6125
Author(s):  
J Pevsner ◽  
V Hou ◽  
A M Snowman ◽  
S H Snyder
Keyword(s):  
Ligand Binding ◽  
Binding Protein ◽  
Protein Characterization ◽  
Odorant Binding Protein ◽  
Odorant Binding

Synthesis and chromatographic characterization of [Tc-99m]technetium-humic acid species

2000 ◽  
Vol 88 (2) ◽  
Author(s):  
D. Rößler ◽  
K. Franke ◽  
R. Süß ◽  
E. Becker ◽  
H. Kupsch
Keyword(s):  
Humic Acid ◽  
Stannous Chloride ◽  
Gel Permeation Chromatography ◽  
Paper Electrophoresis ◽  
Gel Permeation ◽  
Soil Humic Acid ◽  
Chromatographic Characterization ◽  
Ph Dependent ◽  
Layer Chromatography

A natural moor soil humic acid (HA) was labeled with Tc-99m via reduction of pertechnetate with stannous chloride. The humic acid species obtained were characterized by thin layer chromatography (TLC), gel permeation chromatography (GPC), sequential chromatographic analysis (SCA), paper electrophoresis and micropore filtration. Labeling was found to take place in all ranges of molecular weight. Due to the complex humic acid composition and the formation of hydroxo species the labeling yields strongly depend on the separation conditions, ranging from 42% to 80%. The pH-dependent distribution of mobile and immobile species was determined by SCA for HTcO

Characterization of Determinants of Ligand Binding to the Nicotinic Acid Receptor GPR109A (HM74A/PUMA-G)

2005 ◽  
Vol 68 (5) ◽  
pp. 1271-1280 ◽  
Author(s):  
Sorin Tunaru ◽  
Jens Lättig ◽  
Jukka Kero ◽  
Gerd Krause ◽  
Stefan Offermanns
Keyword(s):  
Ligand Binding ◽  
Nicotinic Acid ◽  
Acid Receptor

Characterization of labeled reagents in ligand-binding assays by a surface plasmon resonance biosensor

Bioanalysis ◽  
2017 ◽  
Vol 9 (2) ◽  
pp. 193-207 ◽  
Author(s):  
Jia Duo ◽  
JoAnne Bruno ◽  
Steven Piccoli ◽  
Binodh DeSilva ◽  
Yan J Zhang
Keyword(s):  
Surface Plasmon Resonance ◽  
Ligand Binding ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Binding Assays ◽  
Surface Plasmon Resonance Biosensor
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