Effects of Specific Fatty Acid Acylation of Phospholipase A2 on Its Interfacial Binding and Catalysis

Biochemistry ◽  
1994 ◽  
Vol 33 (38) ◽  
pp. 11598-11607 ◽  
Author(s):  
Zhen Shen ◽  
Shih-Kwang Wu ◽  
Wonhwa Cho
Keyword(s):  
Fatty Acid ◽  
Phospholipase A2 ◽  
Specific Fatty Acid

Cell surface lipids and adhesion. I. The effects of lysophosphatidyl compounds, phospholipase A2 and aggregation-inhibiting protein

1975 ◽  
Vol 18 (3) ◽  
pp. 347-356
Author(s):  
A.S. Curtis ◽  
J. Campbell ◽  
F.M. Shaw
Keyword(s):  
Cell Adhesion ◽  
Fatty Acid ◽  
Cell Surface ◽  
Phospholipase A2 ◽  
Low Temperatures ◽  
Phospholipase A ◽  
Phospholipase Activity ◽  
Surface Lipids ◽  
Parallel Effect ◽  
Inhibiting Protein

Aggregation-inhibiting protein (AIP: Curtis & Greaves, 1965), which diminishes the adhesiveness of cells, particularly at low temperatures, is identified in the present paper as phospholipase A2 (EC. 3.1.1.4). Our reasons for this identification are because phospholipase activity parallels AIP activity on cell adhesion, and because various inhibitors and sera act in a parallel manner on adhesion in the presence of AIP or phospholipase. We suggest that the enzyme acts on adhesion by producing lysolecithin and other lysolipids in the plasmalemma. Addition of lysolipids diminishes cell adhesion in a manner similar to phospholipase A. Incubation of cells in Hanks' medium at 37 degrees C has a parallel effect. Conditions which would be expected to stimulate reacylation of lysolipids in the plasmalemma, i.e. incubation of cells in the external presence of CoA, ATP and a fatty acid, lead to a recovery or maintenance of adhesion after or during Hanks' incubation at 37 degrees C. All these results suggest that lipid components of the cell, probably in the plasmalemma, are of importance in adhesion. The results are discussed in relation to the long-standing controversy about the effects of low temperatures and trypsinization on cell adhesion, for phospholipase treatment of cells affects adhesion in a manner similar to trypsinization.

Elevation of blood lipids in hepatocyte-specific fatty acid transport 4-deficient mice fed with high glucose diets

2019 ◽  
Vol 126 (1) ◽  
pp. 30-38 ◽  
Author(s):  
Stephan Döring ◽  
Jessica Seeßle ◽  
Hongying Gan-Schreier ◽  
Bahador Javaheri ◽  
Li Jiao ◽  
...  
Keyword(s):  
Fatty Acid ◽  
High Glucose ◽  
Blood Lipids ◽  
Fatty Acid Transport ◽  
Acid Transport ◽  
Specific Fatty Acid ◽  
Deficient Mice

scholarly journals Ethanol with a mixed meal increases postprandial triacylglycerol but decreases postprandial non-esterified fatty acid concentrations

2000 ◽  
Vol 83 (6) ◽  
pp. 597-604 ◽  
Author(s):  
Barbara A. Fielding ◽  
Guy Reid Michelle Grady ◽  
Sandy M. Humphreys ◽  
Kevin Evans ◽  
Keith N. Frayn
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Test Meal ◽  
Repeated Measures ◽  
Fatty Acid Content ◽  
Mixed Meal ◽  
Postprandial Lipaemia ◽  
Postprandial Period ◽  
Specific Fatty Acid

Plasma triacylglycerol concentrations increase after the acute ingestion of alcohol (specifically ethanol). However, the effect of ethanol when consumed with a mixed meal has not been well studied. The objective of the present study was to determine the perturbations of lipid metabolism that occur after ingestion of ethanol in combination with a mixed meal of specific fatty acid composition. Blood samples were taken from seven healthy male subjects before and after a mixed meal, with and without ethanol. The specific fatty acid composition of the test meal allowed the fatty acids to be traced into the plasma non-esterified fatty acid pool during the postprandial period. Statistical analysis by repeated measures ANOVA showed significant effects of ethanol. For example, postprandial lipaemia was enhanced after the ethanol test meal compared with the control (P< 0·05), mainly due to increases in triacylglycerol-rich lipoproteins in the flotation range Sf60–400 (VLDL1) (P< 0·05); those in the range Sf20–60 (VLDL2) and also Sf> 400 (chylomicrons) were not significantly affected. The later postprandial increase in plasma non-esterified fatty acid concentrations was reduced after the ingestion of ethanol (P< 0·001), but the proportions of palmitoleic acid (a marker of fatty acid content of the test meal) and of linoleic acid (a marker of endogenous lipolysis) were not affected. The results suggest a primary effect of ethanol on the stimulation of secretion of large VLDL particles, which then compete for clearance with chylomicrons by lipoprotein lipase. The results do not support an effect of ethanol on the release of non-esterified fatty acid into the plasma. The suppression of plasma non-esterified fatty acid concentrations during the postprandial period may contribute towards the beneficial effects of moderate ethanol consumption.

scholarly journals Defining the fetal origin of MLL-AF4 infant leukemia highlights specific fatty acid requirements

Cell Reports ◽  
2021 ◽  
Vol 37 (4) ◽  
pp. 109900
Author(s):  
Vasiliki Symeonidou ◽  
Hélène Jakobczyk ◽  
Salem Bashanfer ◽  
Camille Malouf ◽  
Foteini Fotopoulou ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Specific Fatty Acid ◽  
Fetal Origin ◽  
Infant Leukemia
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