Phosphorescence and Optically Detected Magnetic Resonance Investigation of the Binding of the Nucleocapsid Protein of the Human Immunodeficiency Virus Type 1 and Related Peptides to RNA

Biochemistry ◽  
1994 ◽  
Vol 33 (35) ◽  
pp. 10693-10700 ◽  
Author(s):  
Wai-Chung Lam ◽  
August H. Maki ◽  
Jose R. Casas-Finet ◽  
John W. Erickson ◽  
Bradley P. Kane ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Magnetic Resonance ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Nucleocapsid Protein ◽  
Resonance Investigation ◽  
Immunodeficiency Virus ◽  
Optically Detected ◽  
Magnetic Resonance Investigation

scholarly journals Human Immunodeficiency Virus Type 1 Nucleocapsid Protein Nuclear Localization Mediates Early Viral mRNA Expression

2002 ◽  
Vol 76 (20) ◽  
pp. 10444-10454 ◽  
Author(s):  
Jielin Zhang ◽  
Clyde S. Crumpacker
Keyword(s):  
Human Immunodeficiency Virus ◽  
Mrna Expression ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Nucleocapsid Protein ◽  
Viral Mrna ◽  
Genomic Rna ◽  
Immunodeficiency Virus ◽  
Hiv 1

ABSTRACT An important aspect of the pathophysiology of human immunodeficiency virus type 1 (HIV-1) infection is the ability of the virus to replicate in the host vigorously without a latent phase and to kill cells with a dynamic turnover of 1.8 × 109 cells/day and 10.3 × 109 virions/24 h. The transcription of HIV-1 RNA in acute infection occurs at two stages; the transcription of viral spliced mRNA occurs early, and the transcription of viral genomic RNA occurs later. The HIV-1 Tat protein is translated from the early spliced mRNA and is critical for HIV-1 genomic RNA expression. The cellular transcription factors are important for HIV-1 early spliced mRNA expression. In this study we show that virion nucleocapsid protein (NC) has a role in expression of HIV-1 early spliced mRNA. The HIV-1 NC migrates from the cytoplasm to the nucleus and accumulates in the nucleus at 18 h postinfection. Mutations on HIV-1 NC zinc fingers change the pattern of early viral spliced mRNA expression and result in a delayed expression of early viral mRNA in HIV-infected cells. This delayed HIV-1 early spliced mRNA expression occurs after proviral DNA has been integrated into the cellular genome, as shown by a quantitative integration assay. These results show that virion NC plays an important role in inducing HIV-1 early mRNA expression and contributes to the rapid viral replication that occurs during HIV-1 infection.

The Vpr Protein of Human Immunodeficiency Virus Type 1 Binds to Nucleocapsid Protein p7in Vitro

1996 ◽  
Vol 218 (1) ◽  
pp. 352-355 ◽  
Author(s):  
Ming-Shi Li ◽  
Guillermo Garcia-Asua ◽  
Uma Bhattacharyya ◽  
Paolo Mascagni ◽  
Brian M. Austen ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Nucleocapsid Protein ◽  
Immunodeficiency Virus

Identification of a binding site for the human immunodeficiency virus type 1 nucleocapsid protein

Peptides ◽  
1994 ◽  
pp. 956-957
Author(s):  
K. Sakaguchi ◽  
N. Zambrano ◽  
E. T. Baldwin ◽  
B. A. Shapiro ◽  
J. W. Erickson ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Binding Site ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Nucleocapsid Protein ◽  
Immunodeficiency Virus

scholarly journals Identification of a binding site for the human immunodeficiency virus type 1 nucleocapsid protein.

1993 ◽  
Vol 90 (11) ◽  
pp. 5219-5223 ◽  
Author(s):  
K. Sakaguchi ◽  
N. Zambrano ◽  
E. T. Baldwin ◽  
B. A. Shapiro ◽  
J. W. Erickson ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Binding Site ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Nucleocapsid Protein ◽  
Immunodeficiency Virus
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