Inactivation of Individual Ca2+-Binding Sites in the Paired EF-Hand Sites of Parvalbumin Reveals Asymmetrical Metal-Binding Properties

Biochemistry ◽  
1994 ◽  
Vol 33 (34) ◽  
pp. 10393-10400 ◽  
Author(s):  
Thomas L. Pauls ◽  
Isabelle Durussel ◽  
Martin W. Berchtold ◽  
Jos A. Cox
Keyword(s):  
Metal Binding ◽  
Binding Sites ◽  
Binding Properties ◽  
Ef Hand

scholarly journals Unravelling the Structure of the Tetrahedral Metal-Binding Site in METP3 through an Experimental and Computational Approach

Molecules ◽  
2021 ◽  
Vol 26 (17) ◽  
pp. 5221
Author(s):  
Salvatore La Gatta ◽  
Linda Leone ◽  
Ornella Maglio ◽  
Maria De Fenza ◽  
Flavia Nastri ◽  
...  
Keyword(s):  
Metal Ions ◽  
Binding Site ◽  
Metal Binding ◽  
Binding Sites ◽  
Metal Ion ◽  
Structural Determinants ◽  
Binding Properties ◽  
Tetrahedral Geometry ◽  
Design Synthesis ◽  
Metal Binding Sites

Understanding the structural determinants for metal ion coordination in metalloproteins is a fundamental issue for designing metal binding sites with predetermined geometry and activity. In order to achieve this, we report in this paper the design, synthesis and metal binding properties of METP3, a homodimer made up of a small peptide, which self assembles in the presence of tetrahedrally coordinating metal ions. METP3 was obtained through a redesign approach, starting from the previously developed METP molecule. The undecapeptide sequence of METP, which dimerizes to house a Cys4 tetrahedral binding site, was redesigned in order to accommodate a Cys2His2 site. The binding properties of METP3 were determined toward different metal ions. Successful assembly of METP3 with Co(II), Zn(II) and Cd(II), in the expected 2:1 stoichiometry and tetrahedral geometry was proven by UV-visible spectroscopy. CD measurements on both the free and metal-bound forms revealed that the metal coordination drives the peptide chain to fold into a turned conformation. Finally, NMR data of the Zn(II)-METP3 complex, together with a retrostructural analysis of the Cys-X-X-His motif in metalloproteins, allowed us to define the model structure. All the results establish the suitability of the short METP sequence for accommodating tetrahedral metal binding sites, regardless of the first coordination ligands.

Metal Ion Binding Properties of Perfluorosulfonate Membranes by Laser Excitation Spectroscopy

1988 ◽  
Vol 42 (2) ◽  
pp. 293-295 ◽  
Author(s):  
E. K. L. Wong ◽  
G. L. Richmond
Keyword(s):  
Metal Binding ◽  
Binding Sites ◽  
Metal Ion ◽  
Laser Excitation ◽  
Ion Binding ◽  
Metal Ion Binding ◽  
Binding Properties ◽  
Metal Binding Sites ◽  
Fluorescence Measurements ◽  
Experimental Parameters

The metal ion binding properties of the perfluorosulfonate membrane Nafion® have been investigated in this study. The experiments involve laser-induced fluorescence measurements of europium (III) ions which are bound to the membrane. By the exploitation of the hypersensitivity of the D → F transitions of europium (III) to the ligand binding environment, the properties of the metal binding sites have been analyzed as a function of various experimental parameters. The spectra and fluorescence lifetime measurements provide evidence for distinct metal binding sites within the polymer, each of which is sensitive to the conditions of the membrane preparation.

A grafting approach to obtain site-specific metal-binding properties of EF-hand proteins

2003 ◽  
Vol 16 (6) ◽  
pp. 429-434 ◽  
Author(s):  
Y. Ye ◽  
S. Shealy ◽  
H.-W. Lee ◽  
I. Torshin ◽  
R. Harrison ◽  
...  
Keyword(s):  
Metal Binding ◽  
Binding Properties ◽  
Site Specific ◽  
Ef Hand ◽  
Specific Metal

Characterization of Tescalcin, a Novel EF-Hand Protein with a Single Ca2+-Binding Site:  Metal-Binding Properties, Localization in Tissues and Cells, and Effect on Calcineurin†

Biochemistry ◽  
2003 ◽  
Vol 42 (49) ◽  
pp. 14553-14565 ◽  
Author(s):  
Christina Gutierrez-Ford ◽  
Konstantin Levay ◽  
Aldrin V. Gomes ◽  
Erasmo M. Perera ◽  
Tapan Som ◽  
...  
Keyword(s):  
Binding Site ◽  
Metal Binding ◽  
Binding Properties ◽  
Ef Hand

A new family of fluorescent calcium indicators designed to resist leakage or for measuring calcium near membranes

1994 ◽  
Vol 52 ◽  
pp. 168-169
Author(s):  
Martin Poenie ◽  
Akwasi Minta ◽  
Charles Vorndran
Keyword(s):  
Metal Binding ◽  
Acetoxymethyl Ester ◽  
Binding Properties ◽  
Fura 2 ◽  
New Family ◽  
Anion Transporter ◽  
Calcium Indicator ◽  
Calcium Indicators ◽  
High Calcium ◽  
Intracellular Compartmentalization

The use of fura-2 as an intracellular calcium indicator is complicated by problems of rapid dye leakage and intracellular compartmentalization which is due to a probenecid sensitive anion transporter. In addition there is increasing evidence for localized microdomains of high calcium signals which may not be faithfully reported by fura-2.We have developed a new family of fura-2 analogs aimed at addressing some of these problems. These new indicators are based on a modified bapta which can be readily derivatized to produce fura-2 analogs with a variety of new properties. The modifications do not affect the chromophore and have little impact on the spectral and metal binding properties of the indicator. One of these new derivatives known as FPE3 is a zwitterionic analog of fura-2 that can be loaded into cells as an acetoxymethyl ester and whose retention in cells is much improved. The improved retention of FPE3 is important for both cuvettebased measurements of cell suspensions and for calcium imaging.

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