The RNA-Binding Domain of Transcription Termination Factor Rho:Isolation, Characterization, and Determination of Sequence Limits

Biochemistry ◽  
1994 ◽  
Vol 33 (27) ◽  
pp. 8292-8299 ◽  
Author(s):  
David Modrak ◽  
John P. Richardson
Keyword(s):  
Rna Binding ◽  
Transcription Termination ◽  
Binding Domain ◽  
Termination Factor ◽  
Transcription Termination Factor ◽  
Rna Binding Domain

Crystal structure of the RNA-binding domain from transcription termination factor rho

1998 ◽  
Vol 5 (5) ◽  
pp. 352-356 ◽  
Author(s):  
Timothy J. Allison ◽  
Todd C. Wood ◽  
Deborah M. Briercheck ◽  
Fraydoon Rastinejad ◽  
John P. Richardson ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Rna Binding ◽  
Transcription Termination ◽  
Binding Domain ◽  
Termination Factor ◽  
Transcription Termination Factor ◽  
Rna Binding Domain

scholarly journals Effects of Bicyclomycin on RNA- and ATP-Binding Activities of Transcription Termination Factor Rho

1998 ◽  
Vol 42 (3) ◽  
pp. 571-578 ◽  
Author(s):  
Lucia Carrano ◽  
Cecilia Bucci ◽  
Roberto De Pascalis ◽  
Alfredo Lavitola ◽  
Filomena Manna ◽  
...  
Keyword(s):  
Rna Binding ◽  
Transcription Termination ◽  
Gel Filtration ◽  
Experimental System ◽  
Cross Linking ◽  
Atp Binding ◽  
Mobility Shift ◽  
Termination Factor ◽  
Transcription Termination Factor

ABSTRACT Bicyclomycin is a commercially important antibiotic that has been shown to be effective against many gram-negative bacteria. Genetic and biochemical evidence indicates that the antibiotic interferes with RNA metabolism in Escherichia coli by inhibiting the activity of transcription termination factor Rho. However, the precise mechanism of inhibition is not completely known. In this study we have used in vitro transcription assays to analyze the effects of bicyclomycin on the termination step of transcription. The Rho-dependent transcription termination region located within thehisG cistron of Salmonella typhimurium has been used as an experimental system. The possible interference of the antibiotic with the various functions of factor Rho, such as RNA binding at the primary site, ATP binding, and hexamer formation, has been investigated by RNA gel mobility shift, photochemical cross-linking, and gel filtration experiments. The results of these studies demonstrate that bicyclomycin does not interfere with the binding of Rho to the loading site on nascent RNA. Binding of the factor to ATP is not impeded, on the contrary, the antibiotic appears to decrease the apparent equilibrium dissociation constant for ATP in photochemical cross-linking experiments. The available evidence suggests that this decrease might be due to an interference with the correct positioning of ATP within the nucleotide-binding pocket leading b an inherent block of ATP hydrolysis. Possibly, as a consequence of this interference, the antibiotic also prevents ATP-dependent stabilization of Rho hexamers.

scholarly journals Sequence-specific Interactions in the RNA-binding Domain ofEscherichia coliTranscription Termination Factor Rho

2006 ◽  
Vol 281 (44) ◽  
pp. 33697-33703 ◽  
Author(s):  
T. Kevin Hitchens ◽  
Yiping Zhan ◽  
Lislott V. Richardson ◽  
John P. Richardson ◽  
Gordon S. Rule
Keyword(s):  
Rna Binding ◽  
Binding Domain ◽  
Specific Interactions ◽  
Termination Factor ◽  
Rna Binding Domain

1H, 15N and 13C resonance assignments and secondary structure determination of the RNA-binding domain of E. coli rho protein

1996 ◽  
Vol 8 (4) ◽  
Author(s):  
DeborahM. Briercheck ◽  
TimothyJ. Allison ◽  
JohnP. Richardson ◽  
JefferyF. Ellena ◽  
ToddC. Wood ◽  
...  
Keyword(s):  
Secondary Structure ◽  
Structure Determination ◽  
Rna Binding ◽  
Resonance Assignments ◽  
Binding Domain ◽  
E Coli ◽  
Rho Protein ◽  
Rna Binding Domain
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