Localization of the Heparin-Binding Site of Glia-Derived Nexin/Protease Nexin-1 by Site-Directed Mutagenesis

Biochemistry ◽  
1994 ◽  
Vol 33 (24) ◽  
pp. 7731-7735 ◽  
Author(s):  
Stuart R. Stone ◽  
Marianne L. Brown-Luedi ◽  
Giorgio Rovelli ◽  
Angelo Guidolin ◽  
Elaine McGlynn ◽  
...  
Keyword(s):  
Binding Site ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Heparin Binding ◽  
Heparin Binding Site ◽  
Protease Nexin

scholarly journals Mapping the heparin-binding site of the osteoinductive protein NELL1 by site-directed mutagenesis

FEBS Letters ◽  
2015 ◽  
Vol 589 (24PartB) ◽  
pp. 4026-4032 ◽  
Author(s):  
Kaneyoshi Takahashi ◽  
Arisa Imai ◽  
Masumi Iijima ◽  
Nobuo Yoshimoto ◽  
Andrés D. Maturana ◽  
...  
Keyword(s):  
Binding Site ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Heparin Binding ◽  
Heparin Binding Site

Characterization of the heparin-binding site of glia-derived nexin/protease nexin-1

Biochemistry ◽  
1992 ◽  
Vol 31 (13) ◽  
pp. 3542-3549 ◽  
Author(s):  
Giorgio Rovelli ◽  
Stuart R. Stone ◽  
Angelo Guidolin ◽  
Juerg Sommer ◽  
Denis Monard
Keyword(s):  
Binding Site ◽  
Heparin Binding ◽  
Heparin Binding Site ◽  
Protease Nexin

Mapping the heparin-binding site of the BMP antagonist gremlin by site-directed mutagenesis based on predictive modelling

2015 ◽  
Vol 470 (1) ◽  
pp. 53-64 ◽  
Author(s):  
Arnold Junior Tatsinkam ◽  
Barbara Mulloy ◽  
Christopher C. Rider
Keyword(s):  
Binding Site ◽  
Kidney Development ◽  
Predictive Modelling ◽  
Targeted Mutagenesis ◽  
Site Directed Mutagenesis ◽  
Sulfated Polysaccharides ◽  
Heparin Binding ◽  
Heparin Binding Site ◽  
Foetal Kidney ◽  
Bmp Antagonist

Gremlin, a secreted protein critical in foetal kidney development, is known to bind to the sulfated polysaccharides, heparin and heparan sulfate (HS). In the present study, we employed targeted mutagenesis to map its heparin-binding site. We also demonstrate that gremlin forms non-covalent dimers.

Antithrombin-mediated Inhibition of Factor Vlla-Tissue Factor Complex by the Synthetic Pentasaccharide Representing the Heparin Binding Site to Antithrombin

1996 ◽  
Vol 76 (01) ◽  
pp. 005-008 ◽  
Author(s):  
Jean Claude Lormeau ◽  
Jean Pascal Herault ◽  
Jean Marc Herbert
Keyword(s):  
Binding Site ◽  
Tissue Factor ◽  
Residual Activity ◽  
Coagulation Factors ◽  
Heparin Binding ◽  
Experimental Conditions ◽  
First Order ◽  
Heparin Binding Site ◽  
Coagulant Activity

SummaryWe examined the effect of the synthetic pentasaccharide representing the minimal binding site of heparin to antithrombin on the antithrombin-mediated inactivation of factor Vila bound to tissue factor. This effect was compared to the effect of unfractionated heparin. Using purified recombinant human coagulation factors and either a clotting or an amidolytic assay for the determination of the residual activity of factor Vila, we showed that the pentasaccharide was an efficient antithrombin-dependent inhibitor of the coagulant activity of tissue factor-factor Vila complex. In our experimental conditions, assuming a mean MW of 14,000 for heparin, the molar pseudo-first order rate constants for ATIII-mediated FVIIa inhibition by ATIII-binding heparin and by the synthetic pentasaccharide were found to be similar with respective values of 104,000 ± 10,500 min-1 and 112,000 ± 12,000 min-1 (mean ± s.e.m., n = 3)

scholarly journals Localization of the major heparin-binding site in fibronectin.

1991 ◽  
Vol 266 (12) ◽  
pp. 7812-7818 ◽  
Author(s):  
F J Barkalow ◽  
J E Schwarzbauer
Keyword(s):  
Binding Site ◽  
Heparin Binding ◽  
Heparin Binding Site
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