Mutations affecting Transition-State Stabilization by Residues Coordinating Zinc at the Active Site of Cytidine Deaminase

Biochemistry ◽  
1994 ◽  
Vol 33 (21) ◽  
pp. 6468-6474 ◽  
Author(s):  
Albert A. Smith ◽  
Dean C. Carlow ◽  
Richard Wolfenden ◽  
Steven A. Short
Keyword(s):  
Transition State ◽  
Active Site ◽  
Cytidine Deaminase ◽  
Transition State Stabilization

Functional tyrosine residue in the active center of human dipeptidyl peptidase III

2008 ◽  
Vol 389 (2) ◽  
pp. 163-167 ◽  
Author(s):  
Branka Salopek-Sondi ◽  
Bojana Vukelić ◽  
Jasminka Špoljarić ◽  
Šumski Šimaga ◽  
Dušica Vujaklija ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Transition State ◽  
Active Site ◽  
Functional Role ◽  
Dipeptidyl Peptidase ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Tyrosine Residue ◽  
Transition State Stabilization ◽  
Endogenous Defense

Abstract Human dipeptidyl peptidase III (DPP III) is a member of the metallopeptidase family M49 with an implied role in the pain-modulatory system and endogenous defense against oxidative stress. Here, we report the heterologous expression of human DPP III and the site-directed mutagenesis results which demonstrate a functional role for Tyr318 at the active site of this enzyme. The substitution of Tyr318 to Phe decreased k cat by two orders of magnitude without altering the binding affinity of substrate, or of a competitive hydroxamate inhibitor designed to interact with S1 and S2 subsites. The results indicate that the conserved tyrosine could be involved in transition state stabilization during the catalytic action of M49 peptidases.

scholarly journals Visualization of the Differential Transition State Stabilization within the Active Site Environment

2004 ◽  
Vol 5 (4) ◽  
pp. 186-195 ◽  
Author(s):  
Pawel Kedzierski ◽  
Pawel Wielgus ◽  
Adrian Sikora ◽  
W. Sokalski ◽  
Jerzy Leszczynski
Keyword(s):  
Transition State ◽  
Active Site ◽  
Transition State Stabilization

Transition state stabilization by six arginines clustered in the active site of creatine kinase

2005 ◽  
Vol 1751 (2) ◽  
pp. 178-183 ◽  
Author(s):  
Michael J. Jourden ◽  
Paul R. Geiss ◽  
Michael J. Thomenius ◽  
Lindsay A. Horst ◽  
Melissa M. Barty ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Transition State ◽  
Active Site ◽  
Transition State Stabilization
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