Replacement of the Active-Site Cysteine Residues of DsbA, a Protein Required for Disulfide Bond Formation in vivo

Biochemistry ◽  
1994 ◽  
Vol 33 (7) ◽  
pp. 1907-1914 ◽  
Author(s):  
Andre Zapun ◽  
Leanne Cooper ◽  
Thomas E. Creighton
Keyword(s):  
Disulfide Bond ◽  
Active Site ◽  
Bond Formation ◽  
Cysteine Residues ◽  
Disulfide Bond Formation ◽  
Active Site Cysteine

scholarly journals Intracellular catalysis of disulfide bond formation by the human sulfhydryl oxidase, QSOX1

2007 ◽  
Vol 404 (3) ◽  
pp. 403-411 ◽  
Author(s):  
Seema Chakravarthi ◽  
Catherine E. Jessop ◽  
Martin Willer ◽  
Colin J. Stirling ◽  
Neil J. Bulleid
Keyword(s):  
Disulfide Bond ◽  
Mammalian Cells ◽  
Transmembrane Protein ◽  
Bond Formation ◽  
Secretory Protein ◽  
Lung Fibroblasts ◽  
Carboxypeptidase Y ◽  
Disulfide Bond Formation ◽  
Endometrial Cells

The discovery that the flavoprotein oxidase, Erv2p, provides oxidizing potential for disulfide bond formation in yeast, has led to investigations into the roles of the mammalian homologues of this protein. Mammalian homologues of Erv2p include QSOX (sulfhydryl oxidases) from human lung fibroblasts, guinea-pig endometrial cells and rat seminal vesicles. In the present study we show that, when expressed in mammalian cells, the longer version of human QSOX1 protein (hQSOX1a) is a transmembrane protein localized primarily to the Golgi apparatus. We also present the first evidence showing that hQSOX1a can act in vivo as an oxidase. Overexpression of hQSOX1a suppresses the lethality of a complete deletion of ERO1 (endoplasmic reticulum oxidase 1) in yeast and restores disulfide bond formation, as assayed by the folding of the secretory protein carboxypeptidase Y.

Production of H2O2 in the Endoplasmic Reticulum Promotes In Vivo Disulfide Bond Formation

2012 ◽  
Vol 16 (10) ◽  
pp. 1088-1099 ◽  
Author(s):  
Éva Margittai ◽  
Péter Löw ◽  
Ibolya Stiller ◽  
Alessandra Greco ◽  
Jose Manuel Garcia-Manteiga ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Disulfide Bond ◽  
Bond Formation ◽  
Disulfide Bond Formation
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