Gel Retardation Analysis of the Interaction between C5 Protein and M1 RNA in the Formation of the Ribonuclease P Holoenzyme from Escherichia coli

Biochemistry ◽  
1994 ◽  
Vol 33 (6) ◽  
pp. 1399-1405 ◽  
Author(s):  
Simon J. Talbot ◽  
Sidney Altman
Keyword(s):  
Escherichia Coli ◽  
Ribonuclease P ◽  
Gel Retardation ◽  
M1 Rna

Metal ion requirements and other aspects of the reaction catalyzed by M1 RNA, the RNA subunit of ribonuclease P from Escherichia coli

Biochemistry ◽  
1986 ◽  
Vol 25 (7) ◽  
pp. 1509-1515 ◽  
Author(s):  
Cecilia Guerrier-Takada ◽  
Karen Haydock ◽  
Leland Allen ◽  
Sidney Altman
Keyword(s):  
Escherichia Coli ◽  
Metal Ion ◽  
Ribonuclease P ◽  
M1 Rna

Nucleotide sequence of the gene encoding the RNA subunit (M1 RNA) of ribonuclease P from Escherichia coli

Cell ◽  
1982 ◽  
Vol 30 (2) ◽  
pp. 627-636 ◽  
Author(s):  
Robin E. Reed ◽  
Madeline F. Baer ◽  
Cecilia Guerrier-Takada ◽  
Helen Donis-Keller ◽  
Sidney Altman
Keyword(s):  
Escherichia Coli ◽  
Nucleotide Sequence ◽  
Ribonuclease P ◽  
Gene Encoding ◽  
M1 Rna

The additional guanylate at the 5' terminus of Escherichia coli tRNAHis is the result of unusual processing by RNase P

1986 ◽  
Vol 6 (2) ◽  
pp. 525-529
Author(s):  
O Orellana ◽  
L Cooley ◽  
D Söll
Keyword(s):  
Escherichia Coli ◽  
Rnase P ◽  
E Coli ◽  
Unusual Cleavage ◽  
M1 Rna

In eucaryotes the 5'-terminal guanylate moiety of mature tRNAHis is added posttranscriptionally. To determine whether the same mechanism occurs in procaryotes, we processed in vitro-derived Escherichia coli tRNAHis precursors to mature tRNA, either in E. coli extracts or by using pure M1-RNA, the catalytic component of RNase P. The results show that the extra guanylate at the 5' end of mature E. coli tRNAHis is encoded in the gene and is found in tRNA as the result of an unusual cleavage by RNase P.

Fluorescence properties of a tryptophan residue in an aromatic core of the protein subunit of ribonuclease P from Escherichia coli

1997 ◽  
Vol 267 (4) ◽  
pp. 765-769 ◽  
Author(s):  
Venkat Gopalan ◽  
Ralph Golbik ◽  
Gideon Schreiber ◽  
Alan R. Fersht ◽  
Sidney Altman
Keyword(s):  
Escherichia Coli ◽  
Fluorescence Properties ◽  
Ribonuclease P ◽  
Tryptophan Residue ◽  
Protein Subunit
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