Site-directed mutagenesis on (serine) carboxypeptidase Y. A hydrogen bond network stabilizes the transition state by interaction with the C-terminal carboxylate group of the substrate

Biochemistry ◽  
1994 ◽  
Vol 33 (2) ◽  
pp. 508-517 ◽  
Author(s):  
Uffe H. Mortensen ◽  
S. James Remington ◽  
Klaus Breddam
Keyword(s):  
Hydrogen Bond ◽  
Transition State ◽  
Site Directed Mutagenesis ◽  
Carboxypeptidase Y ◽  
Directed Mutagenesis ◽  
Carboxylate Group ◽  
Hydrogen Bond Network ◽  
Serine Carboxypeptidase ◽  
Bond Network

scholarly journals Structure-Based Site-Directed Mutagenesis of the UDP-MurNAc-Pentapeptide-Binding Cavity of the FemX Alanyl Transferase from Weissella viridescens

2005 ◽  
Vol 187 (11) ◽  
pp. 3833-3838 ◽  
Author(s):  
Antoine P. Maillard ◽  
Sabrina Biarrotte-Sorin ◽  
Régis Villet ◽  
Stéphane Mesnage ◽  
Ahmed Bouhss ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Hydrogen Bond ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Transferase Activity ◽  
Hydrogen Bond Network ◽  
Gram Positive Bacteria ◽  
Binding Cavity ◽  
Bond Network ◽  
Phosphate Groups

ABSTRACT Weissella viridescens FemX (FemXWv) belongs to the Fem family of nonribosomal peptidyl transferases that use aminoacyl-tRNA as the amino acid donor to synthesize the peptide cross-bridge found in the peptidoglycan of many species of pathogenic gram-positive bacteria. We have recently solved the crystal structure of FemXWv in complex with the peptidoglycan precursor UDP-MurNAc-pentapeptide and report here the site-directed mutagenesis of nine residues located in the binding cavity for this substrate. Two substitutions, Lys36Met and Arg211Met, depressed FemXWv transferase activity below detectable levels without affecting protein folding. Analogues of UDP-MurNAc-pentapeptide lacking the phosphate groups or the C-terminal d-alanyl residues were not substrates of the enzyme. These results indicate that Lys36 and Arg211 participate in a complex hydrogen bond network that connects the C-terminal d-Ala residues to the phosphate groups of UDP-MurNAc-pentapeptide and constrains the substrate in a conformation that is essential for transferase activity.

Atomic Dissection of the Hydrogen Bond Network for Transition-State Analogue Binding to Purine Nucleoside Phosphorylase†

Biochemistry ◽  
2002 ◽  
Vol 41 (49) ◽  
pp. 14489-14498 ◽  
Author(s):  
Greg A. Kicska ◽  
Peter C. Tyler ◽  
Gary B. Evans ◽  
Richard H. Furneaux ◽  
Wuxian Shi ◽  
...  
Keyword(s):  
Hydrogen Bond ◽  
Transition State ◽  
Purine Nucleoside Phosphorylase ◽  
Purine Nucleoside ◽  
Hydrogen Bond Network ◽  
Nucleoside Phosphorylase ◽  
Transition State Analogue ◽  
Bond Network

Site-Directed Mutagenesis on (Serine) Carboxypeptidase Y from Yeast. The Significance of Thr60 and Met398 in Hydrolysis and Aminolysis Reactions

1995 ◽  
Vol 117 (22) ◽  
pp. 5944-5950 ◽  
Author(s):  
Steen Bech Sorensen ◽  
Mette Raaschou-Nielsen ◽  
Uffe H. Mortensen ◽  
S. James Remington ◽  
Klaus Breddam
Keyword(s):  
Site Directed Mutagenesis ◽  
Carboxypeptidase Y ◽  
Directed Mutagenesis ◽  
Serine Carboxypeptidase

Halides of Macrocyclic Silver(II) Complexes: Crystal Structures with Hydrogen Bond Network and Reaction Kinetics of the Decomposition

2021 ◽  
pp. 120431
Author(s):  
Akinori Honda ◽  
Shunta Kakihara ◽  
Shuhei Ichimura ◽  
Kazuaki Tomono ◽  
Mina Matsushita ◽  
...  
Keyword(s):  
Hydrogen Bond ◽  
Reaction Kinetics ◽  
Crystal Structures ◽  
Hydrogen Bond Network ◽  
Bond Network ◽  
Kinetics Of

scholarly journals Hydrogen‐Bond Network Determines the Early Photoisomerization of Cph1 and AnPixJ Phytochromes

2021 ◽  
Author(s):  
Xiang-Yang Liu ◽  
Teng-Shuo Zhang ◽  
Qiu Fang ◽  
Wei-Hai Fang ◽  
Leticia González ◽  
...  
Keyword(s):  
Hydrogen Bond ◽  
Hydrogen Bond Network ◽  
Bond Network
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