Carbon-13 NMR analysis of intercompartmental flow of one-carbon units into choline and purines in Saccharomyces cerevisiae

Biochemistry ◽  
1994 ◽  
Vol 33 (1) ◽  
pp. 74-82 ◽  
Author(s):  
Laura B Pasternack ◽  
David A. Laude ◽  
Dean R. Appling
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Nmr Analysis

NMR Analysis of a Multi-domain Peptide of the Saccharomyces cerevisiae Alpha Factor Receptor

2010 ◽  
pp. 80-81
Author(s):  
Racha Estephan ◽  
Jacqueline Englander ◽  
Boris Arshava ◽  
Jeffrey M. Becker ◽  
Fred Naider
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Nmr Analysis ◽  
Alpha Factor ◽  
Domain Peptide

Synthesis and Structure Assignment of 2-(4-Methoxybenzyl)cyclohexyl β-D-Galactopyranoside Stereoisomers

2006 ◽  
Vol 71 (8) ◽  
pp. 1186-1198 ◽  
Author(s):  
David Šaman ◽  
Martina Wimmerová ◽  
Zdeněk Wimmer
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Nmr Analysis ◽  
Enantiomerically Pure ◽  
Structure Assignment

Several promoters were used in the Koenigs-Knorr synthesis of the title alkyl β-D-galactopyranosides, both in their diastereoisomeric forms (5a/5b and 6a/6b), resulting from the synthesis performed with the respective racemic cis and trans isomers of 2-(4-methoxybenzyl)cyclohexan-1-ol, and in their enantiomerically pure forms 5a and 6a, starting only from the (1S,2S)- and (1S,2R)-enantiomers of 2-(4-methoxybenzyl)cyclohexan-1-ol. The aim of the study was to find convenient modification(s) of the Koenigs-Knorr synthesis of alkyl β-D-galactopyranosides from more hindered and more complex 2-substituted cycloalkanols. Separation of the diastereoisomeric compounds using HPLC on a chiral Nucleodex-β-OH column was used to obtain small quantities of all possibly existing enantiomerically pure products for unambiguous structure assignment by NMR analysis. The (1S,2S)- and (1S,2R)- enantiomers of 2-(4-methoxybenzyl)cyclohexan-1-ol (1a and 2a) were prepared by a reduction of 2-(4-methoxybenzyl)cyclohexan-1-one with Saccharomyces cerevisiae in enantiomeric purities: ee = 98.5% ((1S,2S)-enantiomer (1a)), and ee ≥ 99% ((1S,2R)-enantiomer (2a)).

scholarly journals Structural Analysis of Gluco-Oligosaccharides Produced by Leuconostoc lactis and Their Prebiotic Effect

Molecules ◽  
2019 ◽  
Vol 24 (21) ◽  
pp. 3998 ◽  
Author(s):  
Sulhee Lee ◽  
Jisun Park ◽  
Jae-Kweon Jang ◽  
Byung-Hoo Lee ◽  
Young-Seo Park
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Molecular Weight ◽  
Structural Analysis ◽  
Lactobacillus Casei ◽  
Sole Carbon Source ◽  
Carbon Sources ◽  
Nmr Analysis ◽  
Probiotic Strains ◽  
Leuconostoc Lactis ◽  
Modified Media

Leuconostoc lactis CCK940, which exhibits glycosyltransferase activity, produces oligosaccharides using sucrose and maltose as donor and receptor molecules, respectively. The oligosaccharides produced were purified by Bio-gel P2 chromatography and the purified oligosaccharides (CCK-oligosaccharides) consisted of only glucose. 1H-NMR analysis revealed that the CCK-oligosaccharides were composed of 77.6% α-1,6 and 22.4% α-1,4 glycosidic linkages, and the molecular weight of the CCK-oligosaccharides was found to be 9.42 × 102 Da. To determine the prebiotic effect of the CCK-oligosaccharides, various carbon sources were added in modified media. Growth of six probiotic strains, Lactobacillus casei, L. pentosus, L. plantarum, Weissella cibaria, Bifidobacterim animalis, and Saccharomyces cerevisiae, was better when the CCK-oligosaccharides were used as the sole carbon source compared to fructo-oligosaccharides, which are widely used as prebiotics. These results showed that the CCK-oligosaccharides produced from Leu. lactis CCK940 could serve as good candidates for novel prebiotics.

Anti-Saccharomyces cerevisiae Antibodies in Inflammatory Bowel Disease: a Family Study

2001 ◽  
Vol 36 (2) ◽  
pp. 196-201 ◽  
Author(s):  
F. Seibold ◽  
O. Stich ◽  
R. Hufnagl ◽  
S. Kamil ◽  
M. Scheurlen
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Inflammatory Bowel Disease ◽  
Bowel Disease ◽  
Family Study ◽  
Inflammatory Bowel

Immunreaktion auf Saccharomyces cerevisiae Antigene in Patienten mit Morbus Crohn

2007 ◽  
Vol 45 (08) ◽  
Author(s):  
S Schmechel ◽  
V Schachinger ◽  
F Seibold ◽  
C Tillack ◽  
T Ochsenkühn ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Morbus Crohn
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